LOCUS       sequence-86895-e        2294 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytoskeleton, Excitation: 590, Emission: 649.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2294)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 2294)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mPlum-Myotilin-C-14
FEATURES             Location/Qualifiers
     source          1..2294
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             34..711
                     /codon_start=1
                     /product="far-red monomeric derivative of DsRed fluorescent
                     protein (Wang et al., 2004)"
                     /label=mPlum
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKEHMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     RLKVTKGGPLPFAWDILSPQIMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGEFIYKVKVRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEMK
                     MRLRLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRH
                     STGA"
     primer_bind     435..454
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     629..652
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 acgtcagatc cgctagcgct accggtggct accatggtga gcaagggcga ggaggtcatc
       61 aaggagttca tgcgcttcaa ggagcacatg gagggctccg tgaacggcca cgagttcgag
      121 atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccag gctgaaggtg
      181 accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagat catgtacggc
      241 tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc
      301 gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc
      361 caggactcct ccctgcagga cggcgagttc atctacaagg tgaaggtgcg cggcaccaac
      421 ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc ctcctccgag
      481 cggatgtacc ccgaggacgg cgccctgaag ggcgagatga agatgaggct gaggctgaag
      541 gacggcggcc actacgacgc cgaggtcaag accacctaca tggccaagaa gcccgtgcag
      601 ctgcccggcg cctacaagac cgacatcaag ctggacatca cctcccacaa cgaggactac
      661 accatcgtgg aacagtacga gcgcgccgag ggccgccact ccaccggcgc ctccggaagt
      721 gctgggagcg caagcggaag cggaggctcc ggttttaact acgaacgtcc aaaacacttc
      781 atccagtccc aaaacccatg tggctccaga ttgcagcctc ctggaccaga aacctccagc
      841 ttctctagcc agaccaaaca gtcttccatt atcatccagc cccgccagtg tacagagcaa
      901 agattttctg cctcctcaac actgagctct cacatcacca tgtcctcctc tgctttccct
      961 gcttctcccc agcagcatgc tggctccaac ccaggccaaa gggttacaac cacctataac
     1021 cagtccccag ccagcttcct cagctccata ttaccatcac agcctgatta caatagcagt
     1081 aaaatccctt ccgctatgga ttccaactat caacagtcct cagctggcca acctataaat
     1141 gcaaagccat cccaaactgc aaatgctaag cccataccaa gaactcctga tcatgaaata
     1201 caaggatcaa aagaagcttt gattcaagat ttggaaagaa agctgaaatg caaggacacc
     1261 cttcttcata atggaaatca acgtctaaca tatgaagaga agatggctcg cagattgcta
     1321 ggaccacaga atgcagctgc tgtgtttcaa gctcaggatg acagtggtgc acaagactcg
     1381 cagcaacaca actcagaaca tgcgcgactg caagttccta catcacaagt aagaagtaga
     1441 tcaacctcaa ggggagatgt gaatgatcag gatgcaatcc aggagaaatt ttacccacca
     1501 cgtttcattc aagtgccaga gaacatgtcg attgatgaag gaagattctg cagaatggac
     1561 ttcaaagtga gtggactgcc agctcctgat gtgtcatggt atctaaatgg aagaacagtt
     1621 caatcagatg atttgcacaa aatgatagtg tctgagaagg gtcttcattc actcatcttt
     1681 gaagtagtca gagcttcaga tgcaggggct tatgcatgtg ttgccaagaa tagagcagga
     1741 gaagccacct tcactgtgca gctggatgtc cttgcaaaag aacataaaag agcaccaatg
     1801 tttatctaca aaccacagag caaaaaagtt ttagagggag attcagtgaa actagaatgc
     1861 cagatctcgg ctatacctcc accaaagctt ttctggaaaa gaaataatga aatggtacaa
     1921 ttcaacactg accgaataag cttatatcaa gataacactg gaagagttac tttactgata
     1981 aaagatgtaa acaagaaaga tgctgggtgg tatactgtgt cagcagttaa tgaagctgga
     2041 gtgactacat gtaacacaag attagacgtt acggcacgtc caaaccaaac tcttccagct
     2101 cctaagcagt tacgggttcg accaacattc agcaaatatt tagcacttaa tgggaaaggt
     2161 ttgaatgtaa aacaagcttt taacccagaa ggagaatttc agcgtttggc agctcaatct
     2221 ggactctatg aaagtgaaga actttaagga tccaccggat ctagataact gatcataatc
     2281 agccatacca catt
//
