LOCUS       sequence-86903-e        1406 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Ras GTPase, Excitation: 590, Emission: 649.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1406)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 1406)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mPlum-Rab7a-7
FEATURES             Location/Qualifiers
     source          1..1406
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             35..712
                     /codon_start=1
                     /product="far-red monomeric derivative of DsRed fluorescent
                     protein (Wang et al., 2004)"
                     /label=mPlum
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKEHMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     RLKVTKGGPLPFAWDILSPQIMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGEFIYKVKVRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEMK
                     MRLRLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRH
                     STGA"
     primer_bind     436..455
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     630..653
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggtggc taccatggtg agcaagggcg aggaggtcat
       61 caaggagttc atgcgcttca aggagcacat ggagggctcc gtgaacggcc acgagttcga
      121 gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca ggctgaaggt
      181 gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcaga tcatgtacgg
      241 ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc
      301 cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac
      361 ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaaggtgc gcggcaccaa
      421 cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga
      481 gcggatgtac cccgaggacg gcgccctgaa gggcgagatg aagatgaggc tgaggctgaa
      541 ggacggcggc cactacgacg ccgaggtcaa gaccacctac atggccaaga agcccgtgca
      601 gctgcccggc gcctacaaga ccgacatcaa gctggacatc acctcccaca acgaggacta
      661 caccatcgtg gaacagtacg agcgcgccga gggccgccac tccaccggcg cctccggact
      721 cagatctcga gccatgacct ctaggaagaa agtgttgctg aaggttatca tcctgggaga
      781 ttctggagtc gggaagacat cactcatgaa ccagtatgtg aataagaaat tcagcaatca
      841 gtacaaagcc acaataggag ctgactttct gaccaaggag gtgatggtgg atgacaggct
      901 agtcacaatg cagatatggg acacagcagg acaggaacgg ttccagtctc tcggtgtggc
      961 cttctacaga ggtgcagact gctgcgttct ggtatttgat gtgactgccc ccaacacatt
     1021 caaaacccta gatagctgga gagatgagtt tctcatccag gccagtcccc gagatcctga
     1081 aagcttccca tttgttgtgt tgggaaacaa gattgacctc gaaaacagac aagtggccac
     1141 aaagcgggca caggcctggt gctacagcaa aaacaacatt ccctactttg agaccagtgc
     1201 caaggaggcc atcaacgtgg agcaggcgtt ccagacgatt gcacggaatg cacttaagca
     1261 ggaaacggag gtggagctgt acaacgaatt tcctgaacct atcaaactgg acaagaatga
     1321 ccgggccaag gcctcggcag aaagctgcag ttgctgagga tccaccggat ctagataact
     1381 gatcataatc agccatacca catgag
//
