LOCUS       sequence-86926-e        1255 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Mitochondria, Excitation: 440, Emission: 620.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1255)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 1255)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mKeima-Red-Mito-7
FEATURES             Location/Qualifiers
     source          1..1255
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        5..207
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     158..178
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             236..310
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     regulatory      338..347
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(344..346,347..349,350..1012)
                     /codon_start=1
                     /product="monomeric Keima-Red fluorescent protein, also 
                     known as mKeima"
                     /label=mKeima-Red
                     /translation="MVSVIAKQMTYKVYMSGTVNGHYFEVEGDGKGKPYEGEQTVKLTV
                     TKGGPLPFAWDILSPQLQYGSIPFTKYPEDIPDYFKQSFPEGYTWERSMNFEDGAVCTV
                     SNDSSIQGNCFIYNVKISGENFPPNGPVMQKKTQGWEPSTERLFARDGMLIGNDYMALK
                     LEGGGHYLCEFKSTYKAKKPVRMPGRHEIDRKLDVTSHNRDYTSVEQCEIAIARHSLLG
                     "
     polyA_signal    1134..1254
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1171..1190)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 catggtgatg cggtttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
       61 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
      121 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
      181 cggtgggagg tctatataag cagagctggt ttagtgaacc gtcagatccg ctagcatgtc
      241 cgtcctgacg ccgctgctgc tgcggggctt gacaggctcg gcccggcggc tcccagtgcc
      301 gcgcgccaag atccattcgt tgggggatcc accggtcgcc accatggtga gtgtgatcgc
      361 taaacaaatg acctacaagg tttatatgtc aggcacggtc aatggacact actttgaggt
      421 cgaaggcgat ggaaaaggaa agccttacga gggagagcag acagtaaagc tcactgtcac
      481 caagggtgga cctctgccat ttgcttggga tattttatca ccacagcttc agtacggaag
      541 cataccattc accaagtacc ctgaagacat ccctgattat ttcaagcagt cattccctga
      601 gggatataca tgggagagga gcatgaactt tgaagatggt gcagtgtgta ctgtcagcaa
      661 tgattccagc atccaaggca actgtttcat ctacaatgtc aaaatctctg gtgagaactt
      721 tcctcccaat ggacctgtta tgcagaagaa gacacagggc tgggaaccca gcactgagcg
      781 tctctttgca cgagatggaa tgctgatagg aaacgattat atggctctga agttggaagg
      841 aggtggtcac tatttgtgtg aatttaaatc tacttacaag gcaaagaagc ctgtgaggat
      901 gccagggcgc cacgagattg accgcaaact ggatgtaacc agtcacaaca gggattacac
      961 atctgttgag cagtgtgaaa tagccattgc acgccactct ttgctcggtt aagcggccgc
     1021 gactctagat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc
     1081 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt
     1141 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     1201 catttttttc actgcattct agttgtggtt tgtccaactc atcaatgtat cttaa
//
