LOCUS       sequence-8693-ex       12262 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12262)
  AUTHORS   Yu X, Alder JK, Chun JH, Friedman AD, Heimfeld S, Cheng L, Civin CI
  TITLE     HES1 inhibits cycling of hematopoietic progenitor cells via DNA 
            binding.
  JOURNAL   Stem Cells. 2006 Apr . 24(4):876-88.
  PUBMED    16513761
REFERENCE   2  (bases 1 to 12262)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cells.
            2006 Apr . 24(4):876-88."
COMMENT     Sequence Label: EF.mHES1.Ubc.GFP
FEATURES             Location/Qualifiers
     source          1..12262
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     2624..2640
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2770..3948
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3001..3939
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3896..3916
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     3965..3984
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3965..3983
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        4932..5331
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     5979..5998
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      6188..6197
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(6194..6196,6197..6199,6200..6913)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6194..6196,6197..6199,6200..6910)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6239..6260)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6500..6519)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6847..6868
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    6954..7542
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7007..7027)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7425..7436)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7545..7561)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             8067..8247
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8273..8290)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8279..8503
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8549..8977
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8636..8655)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8846..8867
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8986..9006)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8991..9320
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9171..9306
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9233..9252
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9368..9415
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9434..9808
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9938..10059
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9975..9994)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10029..10048
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10108..10124)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10108..10124)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10121..10143)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10132..10148
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10156..10162,10163..10180,10181..10186))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10201..10222
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10339..10356)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10510..11098)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10590..10609)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11269..12060,12061..12129))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11892..11911
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12130..12234)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta agtttaaacg ggccctctag actcgaggtc gacggtatcg
     2641 atcacgagac tagcctcgag aagcttgatg acggtatcga taagctttgc aaagatggat
     2701 aaagttttaa acagagagga atctttgcag ctaatggacc ttctaggtct tgaaaggagt
     2761 gcctcgtgag gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag
     2821 aagttggggg gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg cggggtaaac
     2881 tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat
     2941 ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag
     3001 gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg
     3061 ccttgaatta cttccacctg gctgcagtac gtgattcttg atcccgagct tcgggttgga
     3121 agtgggtggg agagttcgag gccttgcgct taaggagccc cttcgcctcg tgcttgagtt
     3181 gaggcctggc ctgggcgctg gggccgccgc gtgcgaatct ggtggcacct tcgcgcctgt
     3241 ctcgctgctt tcgataagtc tctagccatt taaaattttt gatgacctgc tgcgacgctt
     3301 tttttctggc aagatagtct tgtaaatgcg ggccaagatc tgcacactgg tatttcggtt
     3361 tttggggccg cgggcggcga cggggcccgt gcgtcccagc gcacatgttc ggcgaggcgg
     3421 ggcctgcgag cgcggccacc gagaatcgga cgggggtagt ctcaagctgg ccggcctgct
     3481 ctggtgcctg gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag gctggcccgg
     3541 tcggcaccag ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc agggagctca
     3601 aaatggagga cgcggcgctc gggagagcgg gcgggtgagt cacccacaca aaggaaaagg
     3661 gcctttccgt cctcagccgt cgcttcatgt gactccacgg agtaccgggc gccgtccagg
     3721 cacctcgatt agttctcgag cttttggagt acgtcgtctt taggttgggg ggaggggttt
     3781 tatgcgatgg agtttcccca cactgagtgg gtggagactg aagttaggcc agcttggcac
     3841 ttgatgtaat tctccttgga atttgccctt tttgagtttg gatcttggtt cattctcaag
     3901 cctcagacag tggttcaaag tttttttctt ccatttcagg tgtcgtgagg aattagcttg
     3961 gtactaatac gactcactat agggagaccc aagctggcta gttaagcttg ataattcatg
     4021 ccagctgata taatggagaa aaattcctcc tccccggtgg ctgctacccc agccagtgtc
     4081 aacacgacac cggacaaacc aaagacggcc tctgagcaca gaaagtcatc aaagcctatc
     4141 atggagaaga ggcgaagggc aagaataaat gaaagtctaa gccaactgaa aacactgatt
     4201 ttggatgcac ttaagaaaga tagctcccgg cattccaagc tagagaaggc agacattctg
     4261 gaaatgactg tgaagcacct ccggaacctg cagcgggcgc agatgaccgc cgcgctcagc
     4321 acagacccga gcgtgttggg gaaataccgc gccggcttca gcgagtgcat gaacgaggtg
     4381 acccgcttcc tgtccacgtg tgagggcgtt aacaccgagg tgcgcactcg gctgctgggc
     4441 cacctggcca actgcatgac ccagatcaac gccatgacct accccgggca ggcgcacccc
     4501 gccttgcagg cgccgccgcc gccgcccccg tcaggacctg ccggtcccca gcacgcgcca
     4561 ttcgcgccgc cgccgccgcc gcttgtgccc atccccgggg gcgcggcgcc ccctcccggc
     4621 agcgcaccct gcaagttggg cagccaggct ggagaggctg ccaaggtttt tggcggcttc
     4681 caagtggtgc cggctcctga cggccaattt gcctttctca tccccaacgg ggccttcgct
     4741 cacagcggcc cggtcatccc ggtctacacc agcaacagtg ggacctcggt gggtcctaac
     4801 gcagtgtcac cttccagtgg ctcctcgctc acttcggact ccatgtggag accgtggcgg
     4861 aactgagcgg ccatcgaatt cccaccacac tggactagtg gatccggggg taccgcgtcg
     4921 aggggtgcag cggcctccgc gccgggtttt ggcgcctccc gcgggcgccc ccctcctcac
     4981 ggcgagcgct gccacgtcag acgaagggcg caggagcgtt cctgatcctt ccgcccggac
     5041 gctcaggaca gcggcccgct gctcataaga ctcggcctta gaaccccagt atcagcagaa
     5101 ggacatttta ggacgggact tgggtgactc tagggcactg gttttctttc cagagagcgg
     5161 aacaggcgag gaaaagtagt cccttctcgg cgattctgcg gagggatctc cgtggggcgg
     5221 tgaacgccga tgattatata aggacgcgcc gggtgtggca cagctagttc cgtcgcagcc
     5281 gggatttggg tcgcggttct tgtttgtgga tcgctgtgat cgtcacttgg tgagttgcgg
     5341 gctgctgggc tggccggggc tttcgtggcc gccgggccgc tcggtgggac ggaagcgtgt
     5401 ggagagaccg ccaagggctg tagtctgggt ccgcgagcaa ggttgccctg aactgggggt
     5461 tggggggagc gcacaaaatg gcggctgttc ccgagtcttg aatggaagac gcttgtaagg
     5521 cgggctgtga ggtcgttgaa acaaggtggg gggcatggtg ggcggcaaga acccaaggtc
     5581 ttgaggcctt cgctaatgcg ggaaagctct tattcgggtg agatgggctg gggcaccatc
     5641 tggggaccct gacgtgaagt ttgtcactga ctggagaact cgggtttgtc gtctggttgc
     5701 gggggcggca gttatgcggt gccgttgggc agtgcacccg tacctttggg agcgcgcgcc
     5761 tcgtcgtgtc gtgacgtcac ccgttctgtt ggcttataat gcagggtggg gccacctgcc
     5821 ggtaggtgtg cggtaggctt ttctccgtcg caggacgcag ggttcgggcc tagggtaggc
     5881 tctcctgaat cgacaggcgc cggacctctg gtgaggggag ggataagtga ggcgtcagtt
     5941 tctttggtcg gttttatgta cctatcttct taagtagctg aagctccggt tttgaactat
     6001 gcgctcgggg ttggcgagtg tgttttgtga agttttttag gcaccttttg aaatgtaatc
     6061 atttgggtca atatgtaatt ttcagtgtta gactagtaaa gcttctgcag gtcgactcta
     6121 gaaaattgtc cgctaaattc tggccgtttt tggctttttt gttagacagg atccccgggt
     6181 accggtcgcc accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
     6241 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg
     6301 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
     6361 gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc
     6421 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
     6481 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
     6541 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
     6601 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga
     6661 caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag
     6721 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
     6781 gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg
     6841 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga
     6901 gctgtacaag taaagcggcc gcgactctag aattcgatat caagcttatc gataatcaac
     6961 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     7021 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     7081 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
     7141 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
     7201 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
     7261 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     7321 ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg
     7381 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
     7441 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
     7501 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcatcgatac cgtcgacctc
     7561 gagacctaga aaaacatgga gcaatcacaa gtagcaatac agcagctacc aatgctgatt
     7621 gtgcctggct agaagcacaa gaggaggagg aggtgggttt tccagtcaca cctcaggtac
     7681 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     7741 ggggactgga agggctaatt cactcccaac gaagacaaga tatccttgat ctgtggatct
     7801 accacacaca aggctacttc cctgattggc agaactacac accagggcca gggatcagat
     7861 atccactgac ctttggatgg tgctacaagc tagtaccagt tgagcaagag aaggtagaag
     7921 aagccaatga aggagagaac acccgcttgt tacaccctgt gagcctgcat gggatggatg
     7981 acccggagag agaagtatta gagtggaggt ttgacagccg cctagcattt catcacatgg
     8041 cccgagagct gcatccggac tgtactgggt ctctctggtt agaccagatc tgagcctggg
     8101 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     8161 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     8221 tttagtcagt gtggaaaatc tctagcaggg cccgtttaaa cccgctgatc agcctcgact
     8281 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
     8341 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
     8401 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
     8461 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga
     8521 accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg
     8581 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     8641 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     8701 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     8761 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     8821 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     8881 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     8941 aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt
     9001 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     9061 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     9121 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     9181 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     9241 cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg
     9301 gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc
     9361 agcacgtgtt gacaattaat catcggcata gtatatcggc atagtataat acgacaaggt
     9421 gaggaactaa accatggcca agttgaccag tgccgttccg gtgctcaccg cgcgcgacgt
     9481 cgccggagcg gtcgagttct ggaccgaccg gctcgggttc tcccgggact tcgtggagga
     9541 cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg tccaggacca
     9601 ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg agctgtacgc
     9661 cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg ccatgaccga
     9721 gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc gacccggccg gcaactgcgt
     9781 gcacttcgtg gccgaggagc aggactgaca cgtgctacga gatttcgatt ccaccgccgc
     9841 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca
     9901 gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa
     9961 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
    10021 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac
    10081 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
    10141 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
    10201 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
    10261 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
    10321 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
    10381 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
    10441 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
    10501 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
    10561 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
    10621 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
    10681 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
    10741 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
    10801 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
    10861 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
    10921 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
    10981 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
    11041 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
    11101 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
    11161 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
    11221 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
    11281 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
    11341 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
    11401 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
    11461 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
    11521 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
    11581 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
    11641 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
    11701 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
    11761 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
    11821 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
    11881 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
    11941 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
    12001 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
    12061 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
    12121 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
    12181 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
    12241 tccccgaaaa gtgccacctg ac
//
