LOCUS       sequence-86992-e        1773 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleoli, Excitation: 588, Emission: 635.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Shcherbo D, Murphy CS, Ermakova GV, Solovieva EA, Chepurnykh TV, 
            Shcheglov AS, Verkhusha VV, Pletnev VZ, Hazelwood KL, Roche PM, 
            Lukyanov S, Zaraisky AG, Davidson MW, Chudakov DM
  TITLE     Far-red fluorescent tags for protein imaging in living tissues.
  JOURNAL   Biochem J. 2009 Mar 15;418(3):567-74. doi: 10.1042/BJ20081949.
  PUBMED    19143658
REFERENCE   2  (bases 1 to 1773)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Biochem J"; date: "2009-03-15- 15"; volume: "418"; issue: "3"; 
            pages: "567-74"
COMMENT     Sequence Label: mKate1.3-Fibrillarin-7
FEATURES             Location/Qualifiers
     source          1..1773
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      28..37
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             34..726
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGH"
     CDS             complement(1531..1542)
                     /codon_start=1
                     /gene="cspA"
                     /product="translation enhancing element for E. coli (Qing 
                     et al., 2004)"
                     /label=TEE
                     /translation="NHKV"
ORIGIN
        1 acgtcagatc cgctagcgct accggtagcc accatggtga gcgagctgat taaggagaac
       61 atgcacatga agctgtacat ggagggcacc gtgaacaacc accacttcaa gtgcacatcc
      121 gagggcgaag gcaagcccta cgagggcacc cagaccatga gaatcaaggc ggtcgagggc
      181 ggccctctcc ccttcgcctt cgacatcctg gctaccagct tcatgtacgg cagcaaaacc
      241 ttcatcaacc acacccaggg catccccgac ttctttaagc agtccttccc tgagggcttc
      301 acatgggaga gagtcaccac atacgaagac gggggcgtgc tgaccgctac ccaggacacc
      361 agcctccagg acggctgcct catctacaac gtcaagatca gaggggtgaa cttcccatcc
      421 aacggccctg tgatgcagaa gaaaacactc ggctgggagg cctccaccga gaccctgtac
      481 cccgctgacg gcggcctgga aggcagagcc gacatggccc tgaagctcgt gggcgggggc
      541 cacctgatct gcaacttgaa gaccacatac agatccaaga aacccgctaa gaacctcaag
      601 atgcccggcg tctactatgt ggacagaaga ctggaaagaa tcaaggaggc cgacaaagag
      661 acctacgtcg agcagcacga ggtggctgtg gccagatact gcgacctccc tagcaaactg
      721 gggcacaaac ttaattccgg actcagatct cgagccatga agccaggatt cagtccccgt
      781 gggggtggct ttggcggccg agggggcttt ggtgaccgtg gtggtcgtgg aggccgaggg
      841 ggctttggcg ggggccgagg ccaaggcgga ggctttagag gtcgtggacg aggaggaggt
      901 ggaggcggcg gcggcggtgg aggaggagga agaggtggtg gaggcttcca ttttggtggc
      961 aaccggggtc gtggtcgggg aggaaaaaga ggaaaccagt cggggaagaa tgtgatggtg
     1021 gagccgcatc ggcatgaggg tgtcttcatt tgtcggggaa aggaagatgc actggtcacc
     1081 aagaacctgg tccctgggga atcagtttat ggagagaaga gagtctcgat ttcggaagga
     1141 gatgacaaaa ttgagtaccg agcctggaac cccttccgct ccaagcttgc agcagcaatc
     1201 ctgggtggtg tggaccagat ccacatcaaa ccgggggcta aggttctcta cctcggggct
     1261 gcctcgggca ccacggtctc ccatgtctct gacatcgttg gtccggatgg tctagtctat
     1321 gcagtcgagt tctcccaccg ctctggccgt gacctcatta acttggccaa gaagaggacc
     1381 aacatcattc ctgtgatcga ggatgctcga cacccacaca aataccgcat gctcatcgca
     1441 atggtggatg tgatctttgc tgatgtggcc cagccagacc agacccggat tgtggccctg
     1501 aatgcccaca ccttcctgcg taatggagga cactttgtga tttccattaa ggccaactgc
     1561 attgactcca cagcctcagc cgaggccgtg tttgcctccg aagtgaaaaa gatgcaacag
     1621 gagaacatga agccgcagga gcagttgacc cttgagccat atgaaagaga ccatgccgtg
     1681 gtcgtgggag tgtacaggcc accccccaag gtgaagaact gaggatccac cggatctaga
     1741 taactgatca taatcagcca taccacattg aga
//
