LOCUS       sequence-87017-e         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endoplasmic Reticulum, Excitation: 580, Emission: 608.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Shemiakina II, Ermakova GV, Cranfill PJ, Baird MA, Evans RA, 
            Souslova EA, Staroverov DB, Gorokhovatsky AY, Putintseva EV, 
            Gorodnicheva TV, Chepurnykh TV, Strukova L, Lukyanov S, Zaraisky AG,
            Davidson MW, Chudakov DM, Shcherbo D
  TITLE     A monomeric red fluorescent protein with low cytotoxicity.
  JOURNAL   Nat Commun. 2012;3:1204. doi: 10.1038/ncomms2208.
  PUBMED    23149748
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2012"; volume: "3:1204. doi"; pages: " 
            10.1038/ncomms2208"
COMMENT     Sequence Label: FusionRed-CytERM-N-17
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      216..225
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             222..920
                     /codon_start=1
                     /product="monomeric red fluorescent protein optimized for 
                     use as a fusion tag (Shemiakina et al., 2012)"
                     /label=FusionRed
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMPMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     VVEGGPLPFAFDILATSFMYGSRTFIKHPPGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKVRGVNFPANGPVMQKKTLGWEASTETMYPADGGLEGACDMAL
                     KLVGGGHLICNLETTYRSKKPATNLKMPGVYNVDHRLERIKEADDETYVEQHEVAVARY
                     STGGAGDGGK"
ORIGIN
        1 gtgaaccgtc agatccgcta gcgctaccgg actcagatct ggtacccctt gcagcttcca
       61 gttgtcagga agagaaggct cccatggatc ctgtggtggt gctggggctc tgtctctcct
      121 gtttgcttct cctttcactc tggaaacaga gctatggggg agggaagctt cgaattctgc
      181 agtcgacggt accgcgggcc cgggatccac cggtcgccac catggtgagc gagctgatta
      241 aggagaacat gcccatgaag ctgtacatgg agggcaccgt gaacaaccac cacttcaagt
      301 gcacatccga gggcgaaggc aagccctacg agggcaccca gaccatgaga atcaaggtcg
      361 tcgagggcgg ccctctcccc ttcgccttcg acatcctggc taccagcttc atgtacggca
      421 gcagaacctt catcaagcac cctccgggca tccccgactt ctttaagcag tccttccctg
      481 agggcttcac atgggagaga gtcaccacat acgaagacgg gggcgtgctg accgctaccc
      541 aggacaccag cctccaggac ggctgcctca tctacaacgt caaggttaga ggggtgaact
      601 tcccagccaa cggccctgtg atgcagaaga aaacactcgg ctgggaggcc tccaccgaga
      661 cgatgtaccc cgctgacggc ggcctggaag gcgcatgtga catggccctg aagctcgtgg
      721 gcgggggcca cctgatctgc aaccttgaga ccacatacag atccaagaaa cccgctacga
      781 acctcaagat gcccggcgtc tacaacgtgg accacagact ggaaagaatc aaggaggccg
      841 acgatgagac ctacgtcgag cagcacgagg tggctgtggc cagatactct actggtggcg
      901 ctggtgatgg aggtaaatga gcggccgcga ctctagatca taatcagcca taccacattt
      961 gtagaggttt tacttgcttt aaaaaacctc ccac
//
