LOCUS       sequence-87018-e        1560 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane, Excitation: 580, Emission: 608.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Shemiakina II, Ermakova GV, Cranfill PJ, Baird MA, Evans RA, 
            Souslova EA, Staroverov DB, Gorokhovatsky AY, Putintseva EV, 
            Gorodnicheva TV, Chepurnykh TV, Strukova L, Lukyanov S, Zaraisky AG,
            Davidson MW, Chudakov DM, Shcherbo D
  TITLE     A monomeric red fluorescent protein with low cytotoxicity.
  JOURNAL   Nat Commun. 2012;3:1204. doi: 10.1038/ncomms2208.
  PUBMED    23149748
REFERENCE   2  (bases 1 to 1560)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2012"; volume: "3:1204. doi"; pages: " 
            10.1038/ncomms2208"
COMMENT     Sequence Label: FusionRed-Dectin1A-C-10
FEATURES             Location/Qualifiers
     source          1..1560
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      26..35
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             32..727
                     /codon_start=1
                     /product="monomeric red fluorescent protein optimized for 
                     use as a fusion tag (Shemiakina et al., 2012)"
                     /label=FusionRed
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMPMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     VVEGGPLPFAFDILATSFMYGSRTFIKHPPGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKVRGVNFPANGPVMQKKTLGWEASTETMYPADGGLEGACDMAL
                     KLVGGGHLICNLETTYRSKKPATNLKMPGVYNVDHRLERIKEADDETYVEQHEVAVARY
                     STGGAGDGGK"
ORIGIN
        1 gtcagatccg ctagcgctac cggtcgccac catggtgagc gagctgatta aggagaacat
       61 gcccatgaag ctgtacatgg agggcaccgt gaacaaccac cacttcaagt gcacatccga
      121 gggcgaaggc aagccctacg agggcaccca gaccatgaga atcaaggtcg tcgagggcgg
      181 ccctctcccc ttcgccttcg acatcctggc taccagcttc atgtacggca gcagaacctt
      241 catcaagcac cctccgggca tccccgactt ctttaagcag tccttccctg agggcttcac
      301 atgggagaga gtcaccacat acgaagacgg gggcgtgctg accgctaccc aggacaccag
      361 cctccaggac ggctgcctca tctacaacgt caaggttaga ggggtgaact tcccagccaa
      421 cggccctgtg atgcagaaga aaacactcgg ctgggaggcc tccaccgaga cgatgtaccc
      481 cgctgacggc ggcctggaag gcgcatgtga catggccctg aagctcgtgg gcgggggcca
      541 cctgatctgc aaccttgaga ccacatacag atccaagaaa cccgctacga acctcaagat
      601 gcccggcgtc tacaacgtgg accacagact ggaaagaatc aaggaggccg acgatgagac
      661 ctacgtcgag cagcacgagg tggctgtggc cagatactct actggtggcg ctggtgatgg
      721 aggtaaaggt ggaggaggtt ccggactcag atctggcagc agtggaggtg aatatcatcc
      781 tgatttagaa aatttggatg aagatggata tactcaatta cacttcgact ctcaaagcaa
      841 taccaggata gctgttgttt cagagaaagg atcgtgtgct gcatctcctc cttggcgcct
      901 cattgctgta attttgggaa tcctatgctt ggtaatactg gtgatagctg tggtcctggg
      961 taccatggct atttggagat ccaattcagg aagcaacaca ttggagaatg gctactttct
     1021 atcaagaaat aaagagaacc acagtcaacc cacacaatca tctttagaag acagtgtgac
     1081 tcctaccaaa gctgtcaaaa ccacaggggt tctttccagc ccttgtcctc ctaattggat
     1141 tatatatgag aagagctgtt atctattcag catgtcacta aattcctggg atggaagtaa
     1201 aagacaatgc tggcaactgg gctctaatct cctaaagata gacagctcaa atgaattggg
     1261 atttatagta aaacaagtgt cttcccaacc tgataattca ttttggatag gcctttctcg
     1321 gccccagact gaagtaccat ggctctggga ggatggatca acattctctt ctaacttatt
     1381 tcagatcaga accacagcta cccaagaaaa cccatctcca aattgtgtat ggattcacgt
     1441 gtcagtcatt tatgaccaac tgtgtagtgt gccctcatat agtatttgtg agaagaagtt
     1501 ttcaatgtaa gaattctgca gtcgacggta ccgcgggccc gggatccacc ggatctagat
//
