LOCUS       sequence-87054-e        1349 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Centromeres, Excitation: 604, Emission: 659.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1349)
  AUTHORS   Chu J, Haynes RD, Corbel SY, Li P, Gonzalez-Gonzalez E, Burg JS, 
            Ataie NJ, Lam AJ, Cranfill PJ, Baird MA, Davidson MW, Ng HL, Garcia 
            KC, Contag CH, Shen K, Blau HM, Lin MZ
  TITLE     Non-invasive intravital imaging of cellular differentiation with a 
            bright red-excitable fluorescent protein.
  JOURNAL   Nat Methods. 2014 Mar 16. doi: 10.1038/nmeth.2888.
  PUBMED    24633408
REFERENCE   2  (bases 1 to 1349)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2014 Mar 16. doi: 10.1038/nmeth.2888."
COMMENT     Sequence Label: mCardinal-CENPB-N-22
FEATURES             Location/Qualifiers
     source          1..1349
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      14..23
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    527..579
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      587..596
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             593..1327
                     /codon_start=1
                     /product="far-red fluorescent protein derived from 
                     mNeptune, with further red-shifted spectra"
                     /label=mCardinal
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTTEGEGKPYEGTQTQ
                     RIKVVEGGPLPFAFDILATCFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTVTQDTSLQDGCLIYNVKLRGVNFPSNGPVMQKKTLGWEATTETLYPADGGLEGRCD
                     MALKLVGGGHLHCNLKTTYRSKKPAKNLKMPGVYFVDRRLERIKEADNETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
ORIGIN
        1 gtcagatccg ctagccacca tgggccccaa gcggcggcag ctgacgttcc gggagaagtc
       61 gcggatcatc caggaggtgg aggagaaccc ggacctacgc aagggcgaga tcgcgcggcg
      121 cttcaacatc ccgccgtcca cgctgagcac gatcctgaag aacaagcgcg ccatcctggc
      181 gtcggagcgc aagtacgggg tggcctccac ctgccgcaag accaacaagc tgtctcccta
      241 cgacaagctc gagggcttgc tcatcgcctg gttccagcag atccgcgccg ccggcctgcc
      301 ggtcaagggc atcatcctca aggagaaggc gctgcgcata gccgaggagc tgggcatgga
      361 cgacttcacc gcctccaacg gctggctgga ccgcttccgc cggcgccacg gcgtggtgtc
      421 ctgcagcggc gtggcccgcg cccgcgcgcg aaacgctgcc ccccgcaccc cggcggcgcc
      481 tgccagtccg gccgcggtgc cctcggaggg cagtggcggg agcactctcg agctcaagct
      541 tcgaattctg cagtcgacgg taccgcgggc ccgggatcca ccggtcgcca ccatggtgag
      601 caagggcgag gagctgatca aggagaacat gcacatgaag ctgtacatgg aaggcaccgt
      661 gaacaaccac cacttcaagt gcaccaccga aggggagggc aagccctacg agggcaccca
      721 gacccagagg attaaggtgg tggagggagg ccccctgccg ttcgcattcg acatcctggc
      781 cacctgcttt atgtacggga gcaagacctt catcaaccac acccagggca tccccgattt
      841 ctttaagcag tccttccctg agggcttcac atgggagaga gtcaccacat acgaagacgg
      901 gggcgtgctt accgttaccc aggacaccag cctccaggac ggctgcttga tctacaacgt
      961 caagctcaga ggggtgaact tcccatccaa cggccctgtg atgcagaaga aaacactcgg
     1021 ctgggaggcc accaccgaga ccctgtaccc cgctgacggc ggcctggaag gcagatgcga
     1081 catggccctg aagctcgtgg gcgggggcca cctgcactgc aacctgaaga ccacatacag
     1141 atccaagaaa cccgctaaga acctcaagat gcccggcgtc tactttgtgg accgcagact
     1201 ggaaagaatc aaggaggccg acaatgagac ctacgtcgag cagcacgagg tggctgtggc
     1261 cagatactgc gacctcccta gcaaactggg gcacaaactt aatggcatgg acgagctgta
     1321 caagtaagcg gccgcgactc tagatcata
//
