LOCUS       sequence-87057-e        1136 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endoplasmic Reticulum, Excitation: 604, Emission: 659.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Chu J, Haynes RD, Corbel SY, Li P, Gonzalez-Gonzalez E, Burg JS, 
            Ataie NJ, Lam AJ, Cranfill PJ, Baird MA, Davidson MW, Ng HL, Garcia 
            KC, Contag CH, Shen K, Blau HM, Lin MZ
  TITLE     Non-invasive intravital imaging of cellular differentiation with a 
            bright red-excitable fluorescent protein.
  JOURNAL   Nat Methods. 2014 Mar 16. doi: 10.1038/nmeth.2888.
  PUBMED    24633408
REFERENCE   2  (bases 1 to 1136)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2014 Mar 16. doi: 10.1038/nmeth.2888."
COMMENT     Sequence Label: mCardinal-CytERM-N-17
FEATURES             Location/Qualifiers
     source          1..1136
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        9..46
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      267..276
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             273..1007
                     /codon_start=1
                     /product="far-red fluorescent protein derived from 
                     mNeptune, with further red-shifted spectra"
                     /label=mCardinal
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTTEGEGKPYEGTQTQ
                     RIKVVEGGPLPFAFDILATCFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTVTQDTSLQDGCLIYNVKLRGVNFPSNGPVMQKKTLGWEATTETLYPADGGLEGRCD
                     MALKLVGGGHLHCNLKTTYRSKKPAKNLKMPGVYFVDRRLERIKEADNETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
ORIGIN
        1 aaatgggcgt aggcgtgtac ggtgggaggt ctatataagc agagctggtt tagtgaaccg
       61 tcagatccgc tagcgctacc ggactcagat ctggtaccct tgcagcttcc agttgtcagg
      121 aagagaaggc tcccatggat cctgtggtgg tgctggggct ctgtctctcc tgtttgcttc
      181 tcctttcact ctggaaacag agctatgggg gagggaagct tcgaattctg cagtcgacgg
      241 taccgcgggc ccgggatcca ccggtcgcca ccatggtgag caagggcgag gagctgatca
      301 aggagaacat gcacatgaag ctgtacatgg aaggcaccgt gaacaaccac cacttcaagt
      361 gcaccaccga aggggagggc aagccctacg agggcaccca gacccagagg attaaggtgg
      421 tggagggagg ccccctgccg ttcgcattcg acatcctggc cacctgcttt atgtacggga
      481 gcaagacctt catcaaccac acccagggca tccccgattt ctttaagcag tccttccctg
      541 agggcttcac atgggagaga gtcaccacat acgaagacgg gggcgtgctt accgttaccc
      601 aggacaccag cctccaggac ggctgcttga tctacaacgt caagctcaga ggggtgaact
      661 tcccatccaa cggccctgtg atgcagaaga aaacactcgg ctgggaggcc accaccgaga
      721 ccctgtaccc cgctgacggc ggcctggaag gcagatgcga catggccctg aagctcgtgg
      781 gcgggggcca cctgcactgc aacctgaaga ccacatacag atccaagaaa cccgctaaga
      841 acctcaagat gcccggcgtc tactttgtgg accgcagact ggaaagaatc aaggaggccg
      901 acaatgagac ctacgtcgag cagcacgagg tggctgtggc cagatactgc gacctcccta
      961 gcaaactggg gcacaaactt aatggcatgg acgagctgta caagtaagcg gccgcgactc
     1021 tagatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa aacctcccac
     1081 acctccccct gaacctgaaa cataaaatga atgcaattgt gttgtactgt tattgc
//
