LOCUS       sequence-87058-e        1065 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Plasma Membrane, Excitation: 604, Emission: 659.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Chu J, Haynes RD, Corbel SY, Li P, Gonzalez-Gonzalez E, Burg JS, 
            Ataie NJ, Lam AJ, Cranfill PJ, Baird MA, Davidson MW, Ng HL, Garcia 
            KC, Contag CH, Shen K, Blau HM, Lin MZ
  TITLE     Non-invasive intravital imaging of cellular differentiation with a 
            bright red-excitable fluorescent protein.
  JOURNAL   Nat Methods. 2014 Mar 16. doi: 10.1038/nmeth.2888.
  PUBMED    24633408
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2014 Mar 16. doi: 10.1038/nmeth.2888."
COMMENT     Sequence Label: mCardinal-Farnesyl-5
FEATURES             Location/Qualifiers
     source          1..1065
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        18..69
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     20..40
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      108..117
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             114..845
                     /codon_start=1
                     /product="far-red fluorescent protein derived from 
                     mNeptune, with further red-shifted spectra"
                     /label=mCardinal
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTTEGEGKPYEGTQTQ
                     RIKVVEGGPLPFAFDILATCFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTVTQDTSLQDGCLIYNVKLRGVNFPSNGPVMQKKTLGWEATTETLYPADGGLEGRCD
                     MALKLVGGGHLHCNLKTTYRSKKPAKNLKMPGVYFVDRRLERIKEADNETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
ORIGIN
        1 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
       61 agcagagctg gtttagtgaa ccgtcagatc cgctagcgct accggtcgcc accatggtga
      121 gcaagggcga ggagctgatc aaggagaaca tgcacatgaa gctgtacatg gaaggcaccg
      181 tgaacaacca ccacttcaag tgcaccaccg aaggggaggg caagccctac gagggcaccc
      241 agacccagag gattaaggtg gtggagggag gccccctgcc gttcgcattc gacatcctgg
      301 ccacctgctt tatgtacggg agcaagacct tcatcaacca cacccagggc atccccgatt
      361 tctttaagca gtccttccct gagggcttca catgggagag agtcaccaca tacgaagacg
      421 ggggcgtgct taccgttacc caggacacca gcctccagga cggctgcttg atctacaacg
      481 tcaagctcag aggggtgaac ttcccatcca acggccctgt gatgcagaag aaaacactcg
      541 gctgggaggc caccaccgag accctgtacc ccgctgacgg cggcctggaa ggcagatgcg
      601 acatggccct gaagctcgtg ggcgggggcc acctgcactg caacctgaag accacataca
      661 gatccaagaa acccgctaag aacctcaaga tgcccggcgt ctactttgtg gaccgcagac
      721 tggaaagaat caaggaggcc gacaatgaga cctacgtcga gcagcacgag gtggctgtgg
      781 ccagatactg cgacctccct agcaaactgg ggcacaaact taatggcatg gacgagctgt
      841 acaagtccgg actcagatct aagctgaacc ctcctgatga gagtggcccc ggctgcatga
      901 gctgcaagtg tgtgctctcc tgatgagaat tctgcagtcg acggtaccgc gggcccggga
      961 tccaccggat ctagataact gatcataatc agccatacca catttgtaga ggttttactt
     1021 gctttaaaaa cctcccacac ctccccctga acctgaacat aaatg
//
