LOCUS       sequence-87222-e        1065 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endoplasmic Reticulum, Excitation: 514, Emission: 527.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1065)
  TITLE     Davidson Green fluorescent proteins
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Topaz-CytERM-N-17
FEATURES             Location/Qualifiers
     source          1..1065
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      247..256
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(253..255,256..258,259..972)
                     /codon_start=1
                     /product="enhanced yellow variant of GFP"
                     /label=Topaz YFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMRQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(298..319)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(559..578)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     906..927
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tgggaggtct atataagcag agctggttta gtgaacccgt cagatccgct agcgctaccc
       61 ggactcagat ctggtaccct tgcagcttcc agttgtcagg aagagaaggc tcccatggat
      121 cctgtggtgg tgctggggct ctgtctctcc tgtttgcttc tcctttcact ctggaaacag
      181 agctatgggg gagggaagct tcgaattctg cagtcgacgg taccgcgggc ccgggatcca
      241 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      301 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      361 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      421 ccctggccca ccctcgtgac caccttcggc tacggcgtgc agtgcttcgc ccgctacccc
      481 gaccacatgc gccagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      541 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      601 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
      661 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
      721 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
      781 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
      841 cccgacaacc actacctgag ctaccagtcc gccctgagca aagaccccaa cgagaagcgc
      901 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
      961 ctgtacaagt aagcggccgc gactctagat cataatcagc cataccacat ttgtagaggt
     1021 tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaa
//
