LOCUS       sequence-87248-e        2212 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nuclear Pore, Excitation: 530, Emission: 541.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Hoi H, Howe ES, Ding Y, Zhang W, Baird MA, Sell BR, Allen JR, 
            Davidson MW, Campbell RE
  TITLE     An engineered monomeric Zoanthus sp. yellow fluorescent protein.
  JOURNAL   Chem Biol. 2013 Oct 24;20(10):1296-304. doi: 
            10.1016/j.chembiol.2013.08.008. Epub 2013 Oct 3.
  PUBMED    24094838
REFERENCE   2  (bases 1 to 2212)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2013.08.008"; journalName: "Chem Biol"; date: 
            "2013-10-24- 24"; volume: "20"; issue: "10"; pages: "1296-304"
COMMENT     Sequence Label: mPapaya-Nup50-N-10
FEATURES             Location/Qualifiers
     source          1..2212
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      24..33
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1458..1467
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1464..2177
                     /codon_start=1
                     /product="monomeric yellow fluorescent protein derived from
                     the Zoanthus fluorescent protein zFP538"
                     /label=mPapaya1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEGQSKHGLKEEMTVKYHMEGCVNGHKFVITGEGIGNPFKG
                     KQTANLCVIEGGPLPFSEDILSPGFKYGDRIFTEYPQDIVDYFKNSCPAGYTWERSFLF
                     EDGAVCRCNVDITVSEKENCIYHKSIFRGVNFPADGPVMKKMTTNWEASTEKIVPVPKQ
                     GILKGKVKMYLLLKDGGRYHCQFDTVYKAKSVPSKMPEWHFIQHKLLREDRSDAKNQKW
                     QLTEHAIAGMDELYK"
ORIGIN
        1 tgacggtcag atccgctagc gatgccacca tggccaaaag aaatgccgag aaggaactga
       61 cagataggaa ttgggatcaa gaagatgaag ctgaagaggt gggaacattc tccatggcca
      121 gtgaggaagt cttgaagaat agagccataa agaaagcaaa gcgcagaaat gttggatttg
      181 aatctgacac tggaggagcc tttaaaggtt ttaaaggttt ggtggtacct tctggaggag
      241 gacgcttttc tggatttggt agtggcgctg gagggaagcc tctggaagga ctgtcgaatg
      301 gaaacaacat aaccagtgcc cctcccttcg ccagtgcaaa ggcagcggca gatcccaagg
      361 tagcctttgg ttctcttgct gcaaatggcc ctaccacctt ggttgataaa gtttcaaatc
      421 ccaaaactaa tggggacagt cagcagccct cctcctctgg ccttgcttcc agtaaagctt
      481 gtgtcggaaa tgcctatcac aagcagttgg ccgccttgaa ctgctccgtg cgggattgga
      541 tagtgaagca cgtgaataca aaccccctct gtgatctgac acctatcttt aaagactatg
      601 agaaatattt agcaaacatt gaacagcaac acgggaacag tggcaggaat tctgaaagtg
      661 aatctaacaa agtggcagct gaaacacagt ctccttccct ttttggctca acaaaattac
      721 agcaagagtc aacgtttttg tttcatggca acaaaactga agatacacct gacaagaaga
      781 tggaggtggc atctgaaaag aaaacggacc catcatcact aggagcgaca agtgcctcat
      841 ttaatttcgg caagaaagtt gatagctctg ttttgggctc attaagctct gtccccctga
      901 ctggattttc tttctcccct ggaaactcca gtttatttgg caaagatact acccagagta
      961 aaccagtctc ttcaccattt cccactaaac cattggaggg ccaagcagaa ggtgacagtg
     1021 gtgaatgcaa aggtggagat gaagaagaga atgatgagcc acccaaagta gtagttaccg
     1081 aagtaaaaga agaagacgct ttttactcca aaaagtgtaa actgttttac aagaaagaca
     1141 atgagtttaa agagaaaggc ataggtactc tgcatttaaa acctacagca aatcagaaga
     1201 cacagctttt ggtgcgggca gacaccaatt taggcaacat attgctgaac gttctgattc
     1261 cacccaatat gccatgtacg cgaacaggga agaataacgt tcttatcgtc tgtgttccaa
     1321 atccaccaat tgacgagaag aatgccacca tgccagtcac catgttgatt cgggtaaaaa
     1381 ccagcgagga tgcagacgag ttgcacaaaa ttttactgga gaaaaaggat gccagctccg
     1441 gaggggatcc accggtcgcc accatggtga gcaagggcga ggggcaatcg aagcatgggc
     1501 taaaggagga gatgacggtg aagtaccaca tggaaggttg cgttaatggt cacaaatttg
     1561 tcattactgg agagggcatt ggaaaccctt ttaagggtaa gcaaactgca aatttgtgtg
     1621 tgatagaagg aggcccgctg ccgttctcgg aggacatttt aagtccgggt tttaaatatg
     1681 gtgaccggat tttcacagag tacccacagg atattgtaga ttacttcaag aactcatgtc
     1741 cagcgggcta tacgtgggaa aggagcttcc tctttgagga cggagcggtc tgtcgatgca
     1801 acgtggacat aacagtctct gaaaaggaga actgcatcta tcacaaaagt atcttcagag
     1861 gggtgaattt tcccgccgac ggccccgtaa tgaagaaaat gaccactaat tgggaagcta
     1921 gtaccgagaa aattgtgcct gttccaaagc aagggatatt aaaaggaaag gtcaaaatgt
     1981 acctattgct gaaggatggc ggtcgttatc attgccagtt tgatacggta tataaagcta
     2041 aatcagtgcc ctccaagatg ccagaatggc attttataca gcataagctc cttagggagg
     2101 accgctctga tgctaaaaac cagaaatggc aattaacaga acatgcaatc gcaggcatgg
     2161 acgagctgta caagtaagcg gccgcgactc tagatcataa tcagccatac ca
//
