LOCUS       sequence-87266-e        2829 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nuclear Envelope, Excitation: 505, Emission: 515.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2829)
  AUTHORS   Lam AJ, St-Pierre F, Gong Y, Marshall JD, Cranfill PJ, Baird MA, 
            McKeown MR, Wiedenmann J, Davidson MW, Schnitzer MJ, Tsien RY, Lin 
            MZ
  TITLE     Improving FRET dynamic range with bright green and red fluorescent 
            proteins.
  JOURNAL   Nat Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171.
  PUBMED    22961245
REFERENCE   2  (bases 1 to 2829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171."
COMMENT     Sequence Label: Clover-LaminA-C-18
FEATURES             Location/Qualifiers
     source          1..2829
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      28..37
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             34..750
                     /codon_start=1
                     /product="monomeric derivative of the bright green-yellow 
                     fluorescent protein Clover"
                     /label=mClover2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVACFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(79..100)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     687..708
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 acgtcagatc cgctagcgct accggtcgcc accatggtga gcaagggcga ggagctgttc
       61 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
      121 gtccgcggcg agggcgaggg cgatgccacc aacggcaagc tgaccctgaa gttcatctgc
      181 accaccggca agctgcccgt gccctggccc accctcgtga ccaccttcgg ctacggcgtg
      241 gcctgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
      301 cccgaaggct acgtccagga gcgcaccatc tctttcaagg acgacggtac ctacaagacc
      361 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
      421 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaactt caacagccac
      481 aacgtctata tcacggccga caagcagaag aacggcatca aggctaactt caagatccgc
      541 cacaacgttg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
      601 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gccatcagtc cgccctgagc
      661 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
      721 atcactctcg gcatggacga gctgtacaag tccggactca gatctggcag cggtggaggc
      781 agcgcatccg gcggaagcgg aagcgagacc ccgtcccagc ggcgcgccac ccgcagcggg
      841 gcgcaggcca gctccactcc gctgtcgccc acccgcatca cccggctgca ggagaaggag
      901 gacctgcagg agctcaatga tcgcttggcg gtctacatcg accgtgtgcg ctcgctggaa
      961 acggagaacg cagggctgcg ccttcgcatc accgagtctg aagaggtggt cagccgcgag
     1021 gtgtccggca tcaaggccgc ctacgaggcc gagctcgggg atgcccgcaa gacccttgac
     1081 tcagtagcca aggagcgcgc ccgcctgcag ctggagctga gcaaagtgcg tgaggagttt
     1141 aaggagctga aagcgcgcaa taccaagaag gagggtgacc tgatagctgc tcaggctcgg
     1201 ctgaaggacc tggaggctct gctgaactcc aaggaggccg cactgagcac tgctctcagt
     1261 gagaagcgca cgctggaggg cgagctgcat gatctgcggg gccaggtggc caagcttgag
     1321 gcagccctag gtgaggccaa gaagcaactt caggatgaga tgctgcggcg ggtggatgct
     1381 gagaacaggc tgcagaccat gaaggaggaa ctggacttcc agaagaacat ctacagtgag
     1441 gagctgcgtg agaccaagcg ccgtcatgag acccgactgg tggagattga caatgggaag
     1501 cagcgtgagt ttgagagccg gctggcggat gcgctgcagg aactgcgggc ccagcatgag
     1561 gaccaggtgg agcagtataa gaaggagctg gagaagactt attctgccaa gctggacaat
     1621 gccaggcagt ctgctgagag gaacagcaac ctggtggggg ctgcccacga ggagctgcag
     1681 cagtcgcgca tccgcatcga cagcctctct gcccagctca gccagctcca gaagcagctg
     1741 gcagccaagg aggcgaagct tcgagacctg gaggactcac tggcccgtga gcgggacacc
     1801 agccggcggc tgctggcgga aaaggagcgg gagatggccg agatgcgggc aaggatgcag
     1861 cagcagctgg acgagtacca ggagcttctg gacatcaagc tggccctgga catggagatc
     1921 cacgcctacc gcaagctctt ggagggcgag gaggagaggc tacgcctgtc ccccagccct
     1981 acctcgcagc gcagccgtgg ccgtgcttcc tctcactcat cccagacaca gggtgggggc
     2041 agcgtcacca aaaagcgcaa actggagtcc actgagagcc gcagcagctt ctcacagcac
     2101 gcacgcacta gcgggcgcgt ggccgtggag gaggtggatg aggagggcaa gtttgtccgg
     2161 ctgcgcaaca agtccaatga ggaccagtcc atgggcaatt ggcagatcaa gcgccagaat
     2221 ggagatgatc ccttgctgac ttaccggttc ccaccaaagt tcaccctgaa ggctgggcag
     2281 gtggtgacga tctgggctgc aggagctggg gccacccaca gcccccctac cgacctggtg
     2341 tggaaggcac agaacacctg gggctgcggg aacagcctgc gtacggctct catcaactcc
     2401 actggggaag aagtggccat gcgcaagctg gtgcgctcag tgactgtggt tgaggacgac
     2461 gaggatgagg atggagatga cctgctccat caccaccacg gctcccactg cagcagctcg
     2521 ggggaccccg ctgagtacaa cctgcgctcg cgcaccgtgc tgtgcgggac ctgcgggcag
     2581 cctgccgaca aggcatctgc cagcggctca ggagcccagg tgggcggacc catctcctct
     2641 ggctcttctg cctccagtgt cacggtcact cgcagctacc gcagtgtggg gggcagtggg
     2701 ggtggcagct tcggggacaa tctggtcacc cgctcctacc tcctgggcaa ctccagcccc
     2761 cgaacccaga gcccccagaa ctgcagcatc atgtaaggat ccaccggatc tagataactg
     2821 atcataatc
//
