LOCUS       sequence-87269-e        2340 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytoskeleton, Excitation: 505, Emission: 515.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Lam AJ, St-Pierre F, Gong Y, Marshall JD, Cranfill PJ, Baird MA, 
            McKeown MR, Wiedenmann J, Davidson MW, Schnitzer MJ, Tsien RY, Lin 
            MZ
  TITLE     Improving FRET dynamic range with bright green and red fluorescent 
            proteins.
  JOURNAL   Nat Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171.
  PUBMED    22961245
REFERENCE   2  (bases 1 to 2340)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171."
COMMENT     Sequence Label: Clover-Myotilin-C-14
FEATURES             Location/Qualifiers
     source          1..2340
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      33..42
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             39..755
                     /codon_start=1
                     /product="monomeric derivative of the bright green-yellow 
                     fluorescent protein Clover"
                     /label=mClover2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVACFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(84..105)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     692..713
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tagtgacgtc agatccgcta gcgctaccgg tcgccaccat ggtgagcaag ggcgaggagc
       61 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
      121 tcagcgtccg cggcgagggc gagggcgatg ccaccaacgg caagctgacc ctgaagttca
      181 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ttcggctacg
      241 gcgtggcctg cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
      301 ccatgcccga aggctacgtc caggagcgca ccatctcttt caaggacgac ggtacctaca
      361 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
      421 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aacttcaaca
      481 gccacaacgt ctatatcacg gccgacaagc agaagaacgg catcaaggct aacttcaaga
      541 tccgccacaa cgttgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc
      601 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagccat cagtccgccc
      661 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg
      721 ccgggatcac tctcggcatg gacgagctgt acaagtccgg aagtgctggg agcgcaagcg
      781 gaagcggagg ctccggtttt aactacgaac gtccaaaaca cttcatccag tcccaaaacc
      841 catgtggctc cagattgcag cctcctggac cagaaacctc cagcttctct agccagacca
      901 aacagtcttc cattatcatc cagccccgcc agtgtacaga gcaaagattt tctgcctcct
      961 caacactgag ctctcacatc accatgtcct cctctgcttt ccctgcttct ccccagcagc
     1021 atgctggctc caacccaggc caaagggtta caaccaccta taaccagtcc ccagccagct
     1081 tcctcagctc catattacca tcacagcctg attacaatag cagtaaaatc ccttccgcta
     1141 tggattccaa ctatcaacag tcctcagctg gccaacctat aaatgcaaag ccatcccaaa
     1201 ctgcaaatgc taagcccata ccaagaactc ctgatcatga aatacaagga tcaaaagaag
     1261 ctttgattca agatttggaa agaaagctga aatgcaagga cacccttctt cataatggaa
     1321 atcaacgtct aacatatgaa gagaagatgg ctcgcagatt gctaggacca cagaatgcag
     1381 ctgctgtgtt tcaagctcag gatgacagtg gtgcacaaga ctcgcagcaa cacaactcag
     1441 aacatgcgcg actgcaagtt cctacatcac aagtaagaag tagatcaacc tcaaggggag
     1501 atgtgaatga tcaggatgca atccaggaga aattttaccc accacgtttc attcaagtgc
     1561 cagagaacat gtcgattgat gaaggaagat tctgcagaat ggacttcaaa gtgagtggac
     1621 tgccagctcc tgatgtgtca tggtatctaa atggaagaac agttcaatca gatgatttgc
     1681 acaaaatgat agtgtctgag aagggtcttc attcactcat ctttgaagta gtcagagctt
     1741 cagatgcagg ggcttatgca tgtgttgcca agaatagagc aggagaagcc accttcactg
     1801 tgcagctgga tgtccttgca aaagaacata aaagagcacc aatgtttatc tacaaaccac
     1861 agagcaaaaa agttttagag ggagattcag tgaaactaga atgccagatc tcggctatac
     1921 ctccaccaaa gcttttctgg aaaagaaata atgaaatggt acaattcaac actgaccgaa
     1981 taagcttata tcaagataac actggaagag ttactttact gataaaagat gtaaacaaga
     2041 aagatgctgg gtggtatact gtgtcagcag ttaatgaagc tggagtgact acatgtaaca
     2101 caagattaga cgttacggca cgtccaaacc aaactcttcc agctcctaag cagttacggg
     2161 ttcgaccaac attcagcaaa tatttagcac ttaatgggaa aggtttgaat gtaaaacaag
     2221 cttttaaccc agaaggagaa tttcagcgtt tggcagctca atctggactc tatgaaagtg
     2281 aagaacttta aggatccacc ggatctagat aactgatcat aatcagccat accacattgt
//
