LOCUS       sequence-87331-e        2236 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Intermediate Filaments, Excitation: 488, Emission: 
            507.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2236)
  TITLE     Davidson Green fluorescent proteins
REFERENCE   2  (bases 1 to 2236)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: EGFP-Vimentin-7
FEATURES             Location/Qualifiers
     source          1..2236
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1471..1480
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1477..1479,1480..1482,1483..2196)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1477..1479,1480..1482,1483..2193)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1522..1543)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1783..1802)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2130..2151
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 acgtcagatc cgctagcgct accggactca gatctcgagc tcaagcttcg aattcccatg
       61 tccaccaggt ccgtgtcctc gtcctcctac cgcaggatgt tcggcggccc gggcaccgcg
      121 agccggccga gctccagccg gagctacgtg actacgtcca cccgcaccta cagcctgggc
      181 agcgcgctgc gccccagcac cagccgcagc ctctacgcct cgtccccggg cggcgtgtat
      241 gccacgcgct cctctgccgt gcgcctgcgg agcagcgtgc ccggggtgcg gctcctgcag
      301 gactcggtgg acttctcgct ggccgacgcc atcaacaccg agttcaagaa cacccgcacc
      361 aacgagaagg tggagctgca ggagctgaat gaccgcttcg ccaactacat cgacaaggtg
      421 cgcttcctgg agcagcagaa taagatcctg ctggccgagc tcgagcagct caagggccaa
      481 ggcaagtcgc gcctggggga cctctacgag gaggagatgc gggagctgcg ccggcaggtg
      541 gaccagctaa ccaacgacaa agcccgcgtc gaggtggagc gcgacaacct ggccgaggac
      601 atcatgcgcc tccgggagaa attgcaggag gagatgcttc agagagagga agccgaaaac
      661 accctgcaat ctttcagaca ggatgttgac aatgcgtctc tggcacgtct tgaccttgaa
      721 cgcaaagtgg aatctttgca agaagagatt gcctttttga agaaactcca cgaagaggaa
      781 atccaggagc tgcaggctca gattcaggaa cagcatgtcc aaatcgatgt ggatgtttcc
      841 aagcctgacc tcacggctgc cctgcgtgac gtacgtcagc aatatgaaag tgtggctgcc
      901 aagaacctgc aggaggcaga agaatggtac aaatccaagt ttgctgacct ctctgaggct
      961 gccaaccgga acaatgacgc cctgcgccag gcaaagcagg agtccactga gtaccggaga
     1021 caggtgcagt ccctcacctg tgaagtggat gcccttaaag gaaccaatga gtccctggaa
     1081 cgccagatgc gtgaaatgga agagaacttt gccgttgaag ctgctaacta ccaagacact
     1141 attggccgcc tgcaggatga gattcagaat atgaaggagg aaatggctcg tcaccttcgt
     1201 gaataccaag acctgctcaa tgttaagatg gcccttgaca ttgagattgc cacctacagg
     1261 aagctgctgg aaggcgagga gagcaggatt tctctgcctc ttccaaactt ttcctccctg
     1321 aacctgaggg aaactaatct ggattcactc cctctggttg atacccactc aaaaaggaca
     1381 cttctgatta agacggttga aactagagat ggacaggtta tcaacgaaac ttctcagcat
     1441 cacgatgacc ttgaagggga tccaccggtc gccaccatgg tgagcaaggg cgaggagctg
     1501 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
     1561 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
     1621 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc
     1681 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
     1741 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
     1801 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
     1861 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc
     1921 cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc
     1981 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc
     2041 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg
     2101 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
     2161 gggatcactc tcggcatgga cgagctgtac aagtaaagcg gccgcgactc tagatcataa
     2221 tcagccatac cacatt
//
