LOCUS       sequence-87343-e        2310 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Microtubules, Excitation: 488, Emission: 507.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Rizzo MA, Davidson MW, Piston DW
  TITLE     Fluorescent protein tracking and detection: fluorescent protein 
            structure and color variants.
  JOURNAL   Cold Spring Harb Protoc. 2009 Dec;2009(12):pdb.top63. doi: 
            10.1101/pdb.top63.
  PUBMED    20150100
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cold Spring
            Harb Protoc."; date: "2009-12"; volume: "2009(12):pdb.top63. doi"; 
            pages: " 10.1101/pdb.top63"
COMMENT     Sequence Label: EGFP-Tubulin-6
FEATURES             Location/Qualifiers
     source          1..2310
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        27..78
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     29..49
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      117..126
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(123..125,126..128,129..839)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(123..125,126..128,129..839)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(168..189)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(429..448)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     776..797
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag
       61 gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta ccggtcgcca
      121 ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
      181 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
      241 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
      301 ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga
      361 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
      421 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
      481 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
      541 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga
      601 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg
      661 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
      721 actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg
      781 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt
      841 ccggactcag atctcgagtg cgtgagtgca tctccatcca cgttggccag gctggtgtcc
      901 agattggcaa tgcctgctgg gagctctact gcctggaaca cggcatccag cccgatggcc
      961 agatgccaag tgacaagacc attgggggag gagatgactc cttcaacacc ttcttcagtg
     1021 agacgggcgc tggcaagcac gtgccccggg ctgtgtttgt agacttggaa cccacagtca
     1081 ttgatgaagt tcgcactggc acctaccgcc agctcttcca ccctgagcag ctcatcacag
     1141 gcaaggaaga tgctgccaat aactatgccc gagggcacta caccattggc aaggagatca
     1201 ttgaccttgt gttggaccga attcgcaagc tggctgacca gtgcaccggt cttcagggct
     1261 tcttggtttt ccacagcttt ggtgggggaa ctggttctgg gttcacctcc ctgctcatgg
     1321 aacgtctctc agttgattat ggcaagaagt ccaagctgga gttctccatt tacccagcac
     1381 cccaggtttc cacagctgta gttgagccct acaactccat cctcaccacc cacaccaccc
     1441 tggagcactc tgattgtgcc ttcatggtag acaatgaggc catctatgac atctgtcgta
     1501 gaaacctcga tatcgagcgc ccaacctaca ctaaccttaa ccgccttatt agccagattg
     1561 tgtcctccat cactgcttcc ctgagatttg atggagccct gaatgttgac ctgacagaat
     1621 tccagaccaa cctggtgccc tacccccgca tccacttccc tctggccaca tatgcccctg
     1681 tcatctctgc tgagaaagcc taccatgaac agctttctgt agcagagatc accaatgctt
     1741 gctttgagcc agccaaccag atggtgaaat gtgaccctcg ccatggtaaa tacatggctt
     1801 gctgcctgtt gtaccgtggt gacgtggttc ccaaagatgt caatgctgcc attgccacca
     1861 tcaaaaccaa gcgcagcatc cagtttgtgg attggtgccc cactggcttc aaggttggca
     1921 tcaactacca gcctcccact gtggtgcctg gtggagacct ggccaaggta cagagagctg
     1981 tgtgcatgct gagcaacacc acagccattg ctgaggcctg ggctcgcctg gaccacaagt
     2041 ttgacctgat gtatgccaag cgtgcctttg ttcactggta cgtgggtgag gggatggagg
     2101 aaggcgagtt ttcagaggcc cgtgaagata tggctgccct tgagaaggat tatgaggagg
     2161 ttggtgtgga ttctgttgaa ggagagggtg aggaagaagg agaggaatac taaggatcca
     2221 ccggatctag ataactgatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt
     2281 taaaaaacct cccacacctc cccctgaacc
//
