LOCUS       sequence-87344-e        1637 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: MT End Binding Protein, Excitation: 488, Emission: 
            507.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1637)
  TITLE     Davidson Green fluorescent proteins
REFERENCE   2  (bases 1 to 1637)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: EGFP-EB1-8
FEATURES             Location/Qualifiers
     source          1..1637
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     62..78
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     62..78
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     regulatory      902..911
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(908..910,911..913,914..1627)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(908..910,911..913,914..1624)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(953..974)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1214..1233)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1561..1582
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggactc agatctcgag ctccaccgcg gtggcggccg
       61 ctctagaact agtggatcca tggcagtgaa cgtatactca acgtcagtga ccagtgataa
      121 cctaagtcga catgacatgc tggcctggat caatgagtct ctgcagttga atctgacaaa
      181 gatcgaacag ttgtgctcag gggctgcgta ttgtcagttt atggacatgc tgttccctgg
      241 ctccattgcc ttgaagaaag tgaaattcca agctaagcta gaacacgagt acatccagaa
      301 cttcaaaata ttacaagcag gttttaagag aatgggtgtt gacaaaataa ttcctgtgga
      361 caaattagta aaaggaaagt ttcaggacaa ttttgaattc gttcagtggt tcaagaagtt
      421 cttcgatgca aactatgatg gaaaagacta tgaccctgtg gctgctagac aaggtcaaga
      481 aactgcagtg gctccttccc ttgttgctcc agctctgaat aaaccgaaga aacctctcac
      541 ttctagcagt gcagctcccc agaggcccat ctcaacacag agaaccgctg cggctcctaa
      601 ggctggccct ggtgtggtgc gaaagaaccc tggtgtgggc aacggagacg acgaggcagc
      661 tgagttgatg cagcaggtca acgtattgaa acttactgtt gaagacttgg agaaagagag
      721 ggatttctac ttcggaaagc tacggaacat tgaattgatt tgccaggaga acgaggggga
      781 aaacgaccct gtattgcaga ggattgtaga cattctgtat gccacagatg aaggctttgt
      841 gatacctgat gaagggggcc cacaggagga gcaagaagag tatacccggg atccaccggt
      901 cgccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
      961 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
     1021 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
     1081 gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca
     1141 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
     1201 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
     1261 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
     1321 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca
     1381 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca
     1441 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     1501 caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca
     1561 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     1621 caagtaaagc ggccgcg
//
