LOCUS       sequence-87439-e        2173 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Microtubules, Excitation: 406, Emission: 526.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2173)
  TITLE     Davidson Yellow fluorescent proteins
REFERENCE   2  (bases 1 to 2173)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mAmetrine-Tubulin-6
FEATURES             Location/Qualifiers
     source          1..2173
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(35..37,38..40,41..751)
                     /codon_start=1
                     /product="yellow fluorescent protein with a large Stokes 
                     shift, excitable with violet light (Ai et al., 2008)"
                     /label=mAmetrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTSGKLPVPWPTLVTTLSYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GSYRTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNMNVWDAYITADKQKNGIK
                     ANFKIEHNVEDGGVQLADAYQQNTPIGDGSVLLPDNHYLSFQSKLFKDPNEQRDHMVLL
                     EFVTAAGITPGMDELYK"
     primer_bind     complement(80..101)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 accgtcagat ccgctagcgc taccggtcgc caccatggtg agcaagggcg aggagctgtt
       61 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
      121 cgtgcgcggc gagggcgagg gcgatgccac caacggcaag ctgaccctga agttcatctg
      181 cacctccggc aagctgcccg tgccctggcc caccctcgtg accaccctgt cttacggcgt
      241 gcagtgcttc gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
      301 gcccgaaggc tacgtccagg agcgcaccat ctccttcaag gacgacggca gctacaggac
      361 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
      421 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaaca tgaacgtgtg
      481 ggacgcgtat atcacggccg acaagcagaa gaacggcatc aaagcgaact tcaagatcga
      541 gcacaacgtc gaggacggcg gcgtgcagct cgccgacgcg taccagcaga acacccccat
      601 cggcgacggc tccgtgctgc tgcctgacaa ccactacctg agcttccaga gcaagctgtt
      661 caaagacccc aacgagcagc gcgatcacat ggtcctgctg gagttcgtta ccgccgccgg
      721 gatcactccc ggcatggacg agctgtacaa gtccggactc agatctcgag tgcgtgagtg
      781 catctccatc cacgttggcc aggctggtgt ccagattggc aatgcctgct gggagctcta
      841 ctgcctggaa cacggcatcc agcccgatgg ccagatgcca agtgacaaga ccattggggg
      901 aggagatgac tccttcaaca ccttcttcag tgagacgggc gctggcaagc acgtgccccg
      961 ggctgtgttt gtagacttgg aacccacagt cattgatgaa gttcgcactg gcacctaccg
     1021 ccagctcttc caccctgagc agctcatcac aggcaaggaa gatgctgcca ataactatgc
     1081 ccgagggcac tacaccattg gcaaggagat cattgacctt gtgttggacc gaattcgcaa
     1141 gctggctgac cagtgcaccg gtcttcaggg cttcttggtt ttccacagct ttggtggggg
     1201 aactggttct gggttcacct ccctgctcat ggaacgtctc tcagttgatt atggcaagaa
     1261 gtccaagctg gagttctcca tttacccagc accccaggtt tccacagctg tagttgagcc
     1321 ctacaactcc atcctcacca cccacaccac cctggagcac tctgattgtg ccttcatggt
     1381 agacaatgag gccatctatg acatctgtcg tagaaacctc gatatcgagc gcccaaccta
     1441 cactaacctt aaccgcctta ttagccagat tgtgtcctcc atcactgctt ccctgagatt
     1501 tgatggagcc ctgaatgttg acctgacaga attccagacc aacctggtgc cctacccccg
     1561 catccacttc cctctggcca catatgcccc tgtcatctct gctgagaaag cctaccatga
     1621 acagctttct gtagcagaga tcaccaatgc ttgctttgag ccagccaacc agatggtgaa
     1681 atgtgaccct cgccatggta aatacatggc ttgctgcctg ttgtaccgtg gtgacgtggt
     1741 tcccaaagat gtcaatgctg ccattgccac catcaaaacc aagcgcagca tccagtttgt
     1801 ggattggtgc cccactggct tcaaggttgg catcaactac cagcctccca ctgtggtgcc
     1861 tggtggagac ctggccaagg tacagagagc tgtgtgcatg ctgagcaaca ccacagccat
     1921 tgctgaggcc tgggctcgcc tggaccacaa gtttgacctg atgtatgcca agcgtgcctt
     1981 tgttcactgg tacgtgggtg aggggatgga ggaaggcgag ttttcagagg cccgtgaaga
     2041 tatggctgcc cttgagaagg attatgagga ggttggtgtg gattctgttg aaggagaggg
     2101 tgaggaagaa ggagaggaat actaaggatc caccggatct agataactga tcataatcag
     2161 ccataccaca ttg
//
