LOCUS       sequence-87451-e        2556 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nuclear Envelope, Excitation: 516, Emission: 529.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2556)
  TITLE     Davidson Yellow fluorescent proteins
REFERENCE   2  (bases 1 to 2556)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCitrine-LaminB1-10
FEATURES             Location/Qualifiers
     source          1..2556
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      25..34
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(31..33,34..36,37..747)
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(76..97)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(337..356)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     684..705
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tcagatccgc tagcgctacc ggtcgccacc atggtgagca agggcgagga gctgttcacc
       61 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
      121 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc
      181 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccttcggcta cggcctgatg
      241 tgcttcgccc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
      301 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
      361 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
      421 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac
      481 gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac
      541 aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc
      601 gacggccccg tgctgctgcc cgacaaccac tacctgagct accagtccaa gctgagcaaa
      661 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
      721 actctcggca tggacgagct gtacaagtcc ggactcagat ctcgagctca agcttcgatg
      781 gcgactgcga cccccgtgcc gccgcggatg ggcagccgcg ctggcggccc caccacgccg
      841 ctgagcccca cgcgcctgtc gcggctccag gagaaggagg agctgcgcga gctcaatgac
      901 cggctggcgg tgtacatcga caaggtgcgc agcctggaga cggagaacag cgcgctgcag
      961 ctgcaggtga cggagcgcga ggaggtgcgc ggccgtgagc tcaccggcct caaggcgctc
     1021 tacgagaccg agctggccga cgcgcgacgc gcgctcgacg acacggcccg cgagcgcgcc
     1081 aagctgcaga tcgagctggg caagtgcaag gcggaacacg accagctgct cctcaactat
     1141 gctaagaagg aatctgatct taatggcgcc cagatcaagc ttcgagaata tgaagcagca
     1201 ctcaattcga aagatgcagc tcttgctact gcacttggtg acaaaaaaag tttagaggga
     1261 gatttggagg atctgaagga tcagattgcc cagttggaag cctccttagc tgcagccaaa
     1321 aaacagttag cagatgaaac tttacttaaa gtagatttgg agaatcgttg tcagagcctt
     1381 actgaggact tggagtttcg caaaagcatg tatgaagagg agattaacga gaccagaagg
     1441 aagcatgaaa cgcgcttggt agaggtggat tctgggcgtc aaattgagta tgagtacaag
     1501 ctggcgcaag cccttcatga gatgagagag caacatgatg cccaagtgag gctgtataag
     1561 gaggagctgg agcagactta ccatgccaaa cttgagaatg ccagactgtc atcagagatg
     1621 aatacttcta ctgtcaacag tgccagggaa gaactgatgg aaagccgcat gagaattgag
     1681 agcctttcat cccagctttc taatctacag aaagagtcta gagcatgttt ggaaaggatt
     1741 caagaattag aggacttgct tgctaaagaa aaagacaact ctcgtcgcat gctgacagac
     1801 aaagagagag agatggcgga aataagggat caaatgcagc aacagctgaa tgactatgaa
     1861 cagcttcttg atgtaaagtt agccctggac atggaaatca gtgcttacag gaaactctta
     1921 gaaggcgaag aagagaggtt gaagctgtct ccaagccctt cttcccgtgt gacagtatcc
     1981 cgagcatcct caagtcgtag tgtacgtaca actagaggaa agcggaagag ggttgatgtg
     2041 gaagaatcag aggcgagtag tagtgttagc atctctcatt ccgcctcagc cactggaaat
     2101 gtttgcatcg aagaaattga tgttgatggg aaatttatcc gcttgaagaa cacttctgaa
     2161 caggatcaac caatgggagg ctgggagatg atcagaaaaa ttggagacac atcagtcagt
     2221 tataaatata cctcaagata tgtgctgaag gcaggccaga ctgttacaat ttgggctgca
     2281 aacgctggtg tcacagccag ccccccaact gacctcatct ggaagaacca gaactcgtgg
     2341 ggcactggcg aagatgtgaa ggttatattg aaaaattctc agggagagga ggttgctcaa
     2401 agaagtacag tctttaaaac aaccatacct gaagaagagg aggaggagga agaagcagct
     2461 ggagtggttg ttgaggaaga acttttccac cagcagggaa ccccaagagc atccaataga
     2521 agctgtgcaa ttatgtaggg atccaccgga tctaga
//
