LOCUS       sequence-87496-e        1562 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Gap Junctions, Excitation: 515, Emission: 528.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1562)
  TITLE     Davidson Yellow fluorescent proteins
REFERENCE   2  (bases 1 to 1562)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mVenus-Cx26-M-7
FEATURES             Location/Qualifiers
     source          1..1562
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      797..806
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(803..805,806..808,809..1522)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(848..869)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1109..1128)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1456..1477
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 acgtcagatc cgctagcgct accggactca gatctcgagc tcaagcttcg aattcgcggc
       61 tgcacgctcc tcgggacaca gtgccaacca tccagaggac aagatggatt ggggcacact
      121 acagagcatc ctcgggggtg tcaacaagca ctccaccagc attgggaaaa tctggctcac
      181 tgtcctcttc atcttccgca tcatgatcct cgtggtggcc gcgaaggagg tgtggggaga
      241 tgagcaagcc gattttgttt gcaacactct ccagcctggc tgtaagaatg tgtgctacga
      301 ccactacttc cccatctctc acatccggct ctgggctctg cagctgatca tggtgtccac
      361 gccggccctc ctggtagcta tgcacgtggc ctaccggaga cacgaaaaga aacggaagtt
      421 catgaaggga gagataaaga acgagtttaa ggacatcgaa gagatcaaaa cccagaaggt
      481 ccgtatcgaa gggtccctgt ggtggaccta caccaccagc atcttcttcc gggtcatctt
      541 cgaagctgtc ttcatgtatg tcttttacat catgtacaat ggcttcttca tgcagcgtct
      601 ggtgaagtgt aacgcctggc cttgtcccaa tacagtggac tgcttcattt ccaggcccac
      661 agaaaagact gtcttcacgg tgttcatgat ctctgtgtct ggaatttgca tcctgctaaa
      721 catcacagag ctgtgctatc tgttcattag gtattgctca gggaagtcca aaagaccagt
      781 cgcggatcca ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt
      841 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
      901 gggcgagggc gatgccacct acggcaagct gaccctgaag ctgatctgca ccaccggcaa
      961 gctgcccgtg ccctggccca ccctcgtgac caccctgggc tacggcctgc agtgcttcgc
     1021 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
     1081 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
     1141 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     1201 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
     1261 caccgccgac aagcagaaga acggcatcaa ggccaacttc aagatccgcc acaacatcga
     1321 ggacggcggc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     1381 cgtgctgctg cccgacaacc actacctgag ctaccagtcc aagctgagca aagaccccaa
     1441 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
     1501 catggacgag ctgtacaagt aaagcggccg cgactctaga tcataatcag ccataccaca
     1561 tt
//
