LOCUS       sequence-87513-e        4030 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 515, Emission: 528.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4030)
  TITLE     Davidson Yellow fluorescent proteins
REFERENCE   2  (bases 1 to 4030)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mVenus-Vinculin-23
FEATURES             Location/Qualifiers
     source          1..4030
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      19..28
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(25..27,28..30,31..741)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(70..91)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(331..350)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     678..699
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ccgctagcgc taccggtcgc caccatggtg agcaagggcg aggagctgtt caccggggtg
       61 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
      121 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agctgatctg caccaccggc
      181 aagctgcccg tgccctggcc caccctcgtg accaccctgg gctacggcct gcagtgcttc
      241 gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
      301 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
      361 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
      421 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat
      481 atcaccgccg acaagcagaa gaacggcatc aaggccaact tcaagatccg ccacaacatc
      541 gaggacggcg gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
      601 cccgtgctgc tgcccgacaa ccactacctg agctaccagt ccaagctgag caaagacccc
      661 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
      721 ggcatggacg agctgtacaa gtccggactc agatctcgag ctcaagcttc gaattcggga
      781 aactcgagaa caaagcttga aaacggccgc atgccagtgt ttcatacgcg cacgatcgag
      841 agcatcctgg agccggtggc acagcagatc tcccacctgg tgataatgca cgaggagggc
      901 gaggtggacg gcaaagccat tcctgacctc accgcgcccg tggccgccgt gcaggcggcc
      961 gtcagcaacc tcgtccgggt tggaaaagag actgttcaaa ccactgagga tcagattttg
     1021 aagagagata tgccaccagc atttattaag gttgagaatg cttgcaccaa gcttgtccag
     1081 gcagctcaga tgcttcagtc agacccttac tcagtgcctg ctcgagatta tctaattgat
     1141 gggtcaaggg gcatcctctc tggaacatca gacctgctcc ttaccttcga tgaggctgag
     1201 gtccgtaaaa ttattagagt ttgcaaagga attttggaat atcttacagt ggcagaggtg
     1261 gtggagacta tggaagattt ggtcacttac acaaagaatc ttgggccagg aatgactaag
     1321 atggccaaga tgattgacga gagacagcag gagctcactc accaggagca ccgagtgatg
     1381 ttggtgaact cgatgaacac cgtgaaagag ttgctgccag ttctcatttc agctatgaag
     1441 atttttgtaa caactaaaaa ctcaaaaaac caaggcatag aggaagcttt aaaaaatcgc
     1501 aattttactc tagaaaaaat gagtgctgaa attaatgaga taattcgtgt gttacaactc
     1561 acctcttggg atgaagatgc ctgggccagc aaggacactg aagccatgaa gagagcattg
     1621 gcctccatag actccaaact gaaccaggcc aaaggttggc tccgtgaccc tagtgcctcc
     1681 ccaggggatg ctggtgagca ggccatcaga cagatcttag atgaagctgg aaaagttggt
     1741 gaactctgtg caggcaaaga acgcagggag attctgggaa cttgcaaaat gctagggcag
     1801 atgactgatc aagtggctga cctccgtgcc agaggacaag gatcctcacc ggtggccatg
     1861 cagaaagctc agcaggtatc tcagggtctg gatgtgctca cagcaaaagt ggaaaatgca
     1921 gctcgcaagc tggaagccat gaccaactca aagcagagca ttgcaaagaa gatcgatgct
     1981 gctcagaact ggcttgcaga tccaaatggt ggaccggaag gagaagagca gattcgaggt
     2041 gctttggctg aagctcggaa aatagcagaa ttatgtgatg atcctaaaga aagagatgac
     2101 attctacgtt cccttgggga aatatctgct ctgacttcta aattagcaga tctacgaaga
     2161 caggggaaag gagattctcc agaggctcga gccttggcca aacaggtggc cacggccctg
     2221 cagaacctgc agaccaaaac caaccgggct gtggccaaca gcagaccggc caaagcagct
     2281 gtacaccttg agggcaagat tgagcaagca cagcggtgga ttgataatcc cacagtggat
     2341 gaccgtggag tcggtcaggc tgccatccgg gggcttgtgg ccgaagggca tcgtctggct
     2401 aatgttatga tggggcctta tcggcaagat cttctcgcca agtgtgaccg agtggaccag
     2461 ctgacagccc agctggctga cctggctgcc agaggggaag gggagagtcc tcaggcacga
     2521 gcacttgcat ctcagctcca agactcctta aaggatctaa aagctcggat gcaggaggcc
     2581 atgactcagg aagtgtcaga tgttttcagc gataccacaa ctcccatcaa gctgttggca
     2641 gtggcagcca cggcgcctcc tgatgcgcct aacagggaag aggtatttga tgagagggca
     2701 gctaactttg aaaaccattc aggaaagctt ggtgctacgg ccgagaaggc ggctgcggtt
     2761 ggtactgcta ataaatcaac agtggaaggc attcaggcct cagtgaagac ggcccgagaa
     2821 ctcacacccc aggtggtctc ggctgctcgt atcttactta ggaaccctgg aaatcaagct
     2881 gcttatgaac attttgagac catgaagaac cagtggatcg ataatgttga aaaaatgaca
     2941 gggctggtgg acgaagccat tgataccaaa tctctgttgg atgcttcaga agaagcaatt
     3001 aaaaaagacc tggacaagtg caaggtagct atggccaaca ttcagcctca gatgctggtt
     3061 gctggggcaa ccagtattgc tcgtcgggcc aaccggatcc tgctggtggc taagagggag
     3121 gtggagaatt ccgaggatcc caagttccgt gaggctgtga aagctgcctc tgatgaattg
     3181 agcaaaacca tctccccgat ggtgatggat gcaaaagctg tggctggaaa catttccgac
     3241 cctggactgc aaaagagctt cctggactca ggatatcgga tcctgggagc tgtggccaag
     3301 gtcagagaag ccttccaacc tcaggagcct gacttcccgc cgcctccacc agaccttgaa
     3361 caactccgac taacagatga gcttgctcct cccaaaccac ctctgcctga aggtgaggtc
     3421 cctccaccta ggcctccacc accagaggaa aaggatgaag agttccctga gcagaaggcc
     3481 ggggaggtga ttaaccagcc aatgatgatg gctgccagac agctccatga tgaagctcgc
     3541 aaatggtcca gcaagggcaa tgacatcatt gcagcagcca agcgcatggc tctgctgatg
     3601 gctgagatgt ctcggctggt aagagggggc agtggtacca agcgggcact cattcagtgt
     3661 gccaaggaca tcgccaaggc ctcagatgag gtgactcggt tggccaagga ggttgccaag
     3721 cagtgcacag ataaacggat tagaaccaac ctcttacagg tatgtgagcg aatcccaacc
     3781 ataagcaccc agctcaaaat cctgtccaca gtgaaggcca ccatgctggg ccggaccaac
     3841 atcagtgatg aggagtctga gcaggccaca gagatgctgg ttcacaatgc ccagaacctc
     3901 atgcagtctg tgaaggagac tgtgcgggaa gctgaagctg cttcaatcaa aattcgaaca
     3961 gatgctggat ttacactgcg ctgggttaga aagactccct ggtaccagta ggcggccgcg
     4021 actctagatc
//
