LOCUS       sequence-87523-e        1693 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: MT End Binding Protein, Excitation: 513, Emission: 
            527.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1693)
  AUTHORS   Shaner NC, Patterson GH, Davidson MW
  TITLE     Advances in fluorescent protein technology
  JOURNAL   J Cell Sci. 2007 Dec 15;120(Pt 24):4247-60
  PUBMED    18057027
REFERENCE   2  (bases 1 to 1693)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2007-12-15"; volume: "120(Pt 24)"; pages: "4247-6"
COMMENT     Sequence Label: YPet-EB3-7
FEATURES             Location/Qualifiers
     source          1..1693
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      60..69
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      924..933
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(930..932,933..935,936..1649)
                     /codon_start=1
                     /product="yellow fluorescent protein optimized for FRET 
                     with CyPet (Nguyen and Daugherty, 2005)"
                     /label=YPet
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLLCTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALFKDPNEKRDHMVLL
                     EFLTAAGITEGMNELYK"
     primer_bind     complement(1236..1255)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 atccgctagc gctaccggac tcagatctcg agctcaagct tcgaattctg cagtcgactg
       61 ccaccatggc cgtcaatgtg tactccacat ctgtgaccag tgaaaatctg agtcgccatg
      121 atatgcttgc atgggtcaac gactccctgc acctcaacta taccaagata gaacagcttt
      181 gttcaggggc agcctactgc cagttcatgg acatgctctt ccccggctgt gtgcacttga
      241 ggaaagtgaa gttccaggcc aaactagagc atgaatacat ccacaacttc aaggtgctgc
      301 aagcagcttt caagaagatg ggtgttgaca aaatcattcc tgtagagaaa ttagtgaaag
      361 gaaaattcca agataatttt gagtttattc agtggtttaa gaaattcttt gacgcaaact
      421 atgatggaaa ggattacaac cctctgctgg cgcggcaggg ccaggacgta gcgccacctc
      481 ctaacccagg tgatcagatc ttcaacaaat ccaagaaact cattggcaca gcagttccac
      541 agaggacgtc ccccacaggc ccaaaaaaca tgcagacctc tggccggctg agcaatgtgg
      601 cccccccctg cattctccgg aagaatcctc catcagcccg aaatggcggc catgagactg
      661 atgcccaaat tcttgaactc aaccaacagc tggtggactt gaagctgaca gtggatgggc
      721 tggagaagga acgtgacttc tacttcagca aacttcgtga catcgagctc atctgccagg
      781 agcatgaaag tgaaaacagc cctgttatct caggcatcat tggcatcctc tatgccacag
      841 aggaaggatt cgcaccccct gaggacgatg agattgaaga gcatcaacaa gaagaccagg
      901 acgagtacct ggatccaccg gtcgccacca tggtgagcaa aggcgaagag ctgttcaccg
      961 gcgtggtgcc catcctggtg gagctggacg gcgacgtgaa cggccacaag ttcagcgtga
     1021 gcggcgaggg cgagggcgac gccacctacg gcaagctgac cctgaagctg ctgtgcacca
     1081 ccggcaagct gcccgtgccc tggcccaccc tggtgaccac cctgggctac ggcgtgcagt
     1141 gcttcgcccg gtaccccgac cacatgaagc agcacgactt cttcaagagc gccatgcccg
     1201 agggctacgt gcaggagcgg accatcttct tcaaggacga cggcaactac aagacccggg
     1261 ccgaggtgaa gttcgagggc gacaccctgg tgaaccggat cgagctgaag ggcatcgact
     1321 tcaaggagga cggcaacatc ctgggccaca agctggagta caactacaac agccacaacg
     1381 tgtacatcac cgccgacaag cagaagaacg gcatcaaggc caacttcaag atccggcaca
     1441 acatcgagga cggcggcgtg cagctggccg accactacca gcagaacacc cccatcggcg
     1501 acggccccgt gctgctgccc gacaaccact acctgagcta ccagagcgcc ctgttcaagg
     1561 accccaacga gaagcgggac cacatggtgc tgctggagtt cctgaccgcc gccggcatca
     1621 ccgagggcat gaacgagctc tataagtaaa gcggccgcga ctctagatca taatcagcca
     1681 taccacattg aga
//
