LOCUS       sequence-87571-e         829 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 829)
  TITLE     Scott Lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pUAST tdTomato Profilin WT
FEATURES             Location/Qualifiers
     source          1..829
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    50..74
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(459..483)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     intron          576..641
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             771..791
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ctcagcttcg aattctgcag tcgacggtac cgcgggcccg ggatcatcaa caagtttgta
       61 caaaaaagca ggcttaatga gctggcaaga ttatgtggac aaccaactcc tggcctcgca
      121 gtgcgtgacc aaggcgtgca tcgccggcca cgacggcaac atttgggcgc agtccagtgg
      181 ctttgaggtg acaaaagagg agctctccaa actgatcagc ggctttgacc agcaggacgg
      241 tctcaccagc aacggcgtga cactcgccgg ccagcggtac atttaccttt ccggcacaga
      301 ccgcgtggtg cgcgccaagc tcggccggag cggagtgcac tgcatgaaga caacacaagc
      361 cgtgatcgtt tccatctacg aggatcccgt tcagccccag caggccgctt ccgtggtaga
      421 gaaacttgga gattatctga ttacttgcgg gtactagaac ccagctttct tgtacaaagt
      481 ggttgatgat cctctagagg atctttgtga aggaacctta cttctgtggt gtgacataat
      541 tggacaaact acctacagag atttaaagct ctaaggtaaa tataaaattt ttaagtgtat
      601 aatgtgttaa actactgatt ctaattgttt gtgtatttta gattccaacc tatggaactg
      661 atgaatggga gcagtggtgg aatgccttta atgaggaaaa cctgttttgc tcagaagaaa
      721 tgccatctag tgatgatgag gctactgctg actctcaaca ttctactcct ccaaaaaaga
      781 agagaaaggt agaagacccc aaggactttc cttcagaatt gctaagttt
//
