LOCUS       sequence-87591-e         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses His-tagged NELF-E-mOrange fusion protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Tome JM, Ozer A, Pagano JM, Gheba D, Schroth GP, Lis JT
  TITLE     Comprehensive analysis of RNA-protein interactions by 
            high-throughput sequencing-RNA affinity profiling.
  JOURNAL   Nat Methods. 2014 Jun;11(6):683-8. doi: 10.1038/nmeth.2970. Epub 
            2014 May 8.
  PUBMED    24809628
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2970"; journalName: "Nat Methods"; date: "2014-06"; 
            volume: "11"; issue: "6"; pages: "683-8"
COMMENT     Sequence Label: NELFE-mOrange pHis-//
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(45..92)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     88..106
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(159..176)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gggcgcgtcc cattcgccaa tccggatata gttcctcctt tcagcaaaaa acccctcaag
       61 acccgtttag aggccccaag gggttatgct agttattgct cagcggtggc agcagccaac
      121 tcagcttcct ttcgggcttt gttagcagcc ggatctcagt ggtggtggtg gtggtgctcg
      181 agtgcggccg caagcttggt accgcatgcc tcgagactgc aggctctaga ttcgaaagcg
      241 gccgcactag tctagagcaa gaagtcttca tcgtattgaa ccatctcgcg gtggtccttg
      301 tggctaccct tttgtgattt gctggcggca atagatgacc agactgccga cgaggaggca
      361 tcgttaatgg gctcaatctg gggctggcgt ctagccaact gcacctgcaa gttgatgcca
      421 ttcacattct tgccatgaat ctccgcaatg gcgcgatctg ccgactcggg tttggcgaag
      481 gaaacaaagc cgcgactctt ttcgatctcc atggagacat tgacaatggt gccgtagtcg
      541 ttgaatgtct tttttaagaa atcctcggtg accttgttgc cggacacgaa gatggtattg
      601 ccggcccgtg gcttctctgg ctgcgctgtg tccatgggca ccttctcggg catgccgcgc
      661 tcctctcgat cccgctcggt ggcgaagtgt tggtagagat tctgtcgacg cggctcctcc
      721 ttaatgatct cactaatgat cgtctcctgg gccacgtcgt tctgcgtaga cgaaaagggc
      781 tggtaggagg caacagtggt ttccgaggtg gagcgcttgg ctctttcctg tcctttgggc
      841 cgcttgaagg
//
