LOCUS       sequence-87592-e         876 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses His-tagged NELF-E-mOrange fusion protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Tome JM, Ozer A, Pagano JM, Gheba D, Schroth GP, Lis JT
  TITLE     Comprehensive analysis of RNA-protein interactions by 
            high-throughput sequencing-RNA affinity profiling.
  JOURNAL   Nat Methods. 2014 Jun;11(6):683-8. doi: 10.1038/nmeth.2970. Epub 
            2014 May 8.
  PUBMED    24809628
REFERENCE   2  (bases 1 to 876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2970"; journalName: "Nat Methods"; date: "2014-06"; 
            volume: "11"; issue: "6"; pages: "683-8"
COMMENT     Sequence Label: NELFE-mOrange pHis-//
FEATURES             Location/Qualifiers
     source          1..876
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    72..96
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             111..133
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             153..170
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             192..212
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     635..654
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa attaatacga
       61 ctcactatag gggaattgtg agcggataac aattcccctc tagaaataat tttgtttaac
      121 tttaagaagg agatatacat atgtcgtact accatcacca tcaccatcac gattacgata
      181 tcccaacgac cgaaaacctg tattttcagg gcgccatgga tctggtgagc aagggcgagg
      241 agaataacat ggccatcatc aaggagttca tgcgcttcaa ggtgcgcatg gagggctccg
      301 tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggctttc
      361 agaccgctaa gctgaaggtg accaagggtg gccccctgcc cttcgcctgg gacatcctgt
      421 cccctcagtt cacctacggc tccaaggcct acgtgaagca ccccgccgac atccccgact
      481 acttcaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg
      541 gcggcgtggt gaccgtgacc caggactcct ccctccagga cggcgagttc atctacaagg
      601 tgaagctgcg cggcaccaac ttcccctccg acggccccgt aatgcagaag aagaccatgg
      661 gctgggaggc ctcctccgag cggatgtacc ccgaggacgg cgccctgaag ggcgagatca
      721 agatgaggct gaagctgaag gacggcggcc actacacctc cgaggtcaag accacctaca
      781 aggccaagaa gcccgtgcag ctgcccggcg cctacatcgt cggcatcaag ttggacatca
      841 cctcccacaa cgaggactac accatcgtgg aacagt
//
