LOCUS       sequence-87638-e         899 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses HA tagged chimeric human MEK1 (nucleotides 1–201)-MEK5 
            (498–1344 with S311DT315D) from puromycin resistance retroviral 
            vector .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 899)
  AUTHORS   Brady DC, Crowe MS, Turski ML, Hobbs GA, Yao X, Chaikuad A, Knapp S,
            Xiao K, Campbell SL, Thiele DJ, Counter CM
  TITLE     Copper is required for oncogenic BRAF signalling and tumorigenesis.
  JOURNAL   Nature. 2014 Apr 9. doi: 10.1038/nature13180.
  PUBMED    24717435
REFERENCE   2  (bases 1 to 899)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2014 Apr 9. doi: 10.1038/nature13180."
COMMENT     Sequence Label: pBABEpuro-HA-MEK1-MEK5DD
FEATURES             Location/Qualifiers
     source          1..899
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tcacgggggc ccctgctgct ggctccgcct ctcctccagc gcccggcaca cccacatgga
       61 caccacggcg gcatttccat cattgaactg cacgatgaac gggtggccca tcaattcttc
      121 aggtgctggc ctttcttttg gctgttttcg catacactga gtgatgaaat gtacaaatgg
      181 ctccgagaac tctccaactg gaaggacggg cgaatcctca tcaacaatgc actgcagaag
      241 ctggagaggc attaaagatc cctggttttt ctgaatctga ggatatggaa acctcccaag
      301 agcaagctcc ataaaagaga ttcctaagct ccagacatca gaatgaattc catactgctc
      361 ccctgaaatc ctttcaggcg ccatataagc atttgttcca acatagtcct tggctatatc
      421 attcaccagc tgagtgctaa ctccaaaatc acacagctta acctgtcctc ttgtgtttac
      481 tagcatattg gagggcttca cgtctctatg taaaatcttt aaactccaca aataagtaag
      541 gcctttaaca actgctactg caattcttcc aaggacatgt tctggcattt tcctatatac
      601 atccaaagat cccccatcca tgaattctgt acatattgaa atcctgtttt ctacaaaaaa
      661 tgctccataa aatccaatga tatatgatga atcgcactta taaagaattt ccaattcaga
      721 cataatttgc ttctgaagtt ccagtgtaat atctagtagt atgaccttta cagctaatat
      781 tttcccactc gggacatgat atgctttgta gactgtgcct ccgttgccat gaccaagagt
      841 gtcccgatat cgtatgtcgt catccttcag ttctcccacc ttctgcttct gggtaagaa
//
