LOCUS       sequence-87674-e        1065 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses HA tagged human ERK2 D321N from blasticidin resistance 
            retroviral vector .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Brady DC, Crowe MS, Turski ML, Hobbs GA, Yao X, Chaikuad A, Knapp S,
            Xiao K, Campbell SL, Thiele DJ, Counter CM
  TITLE     Copper is required for oncogenic BRAF signalling and tumorigenesis.
  JOURNAL   Nature. 2014 Apr 9. doi: 10.1038/nature13180.
  PUBMED    24717435
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2014 Apr 9. doi: 10.1038/nature13180."
COMMENT     Sequence Label: pWZLblasti-HA-ERK2 D321N
FEATURES             Location/Qualifiers
     source          1..1065
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     7..23
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             112..138
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 tcctcccttt atccagccct cactccttct ctaggcgccg gccggatcca gaattcactc
       61 gagatacgta aaacaattga agatctagtc gacgatatcg ccgccaccat gtacccatac
      121 gatgttccag attacgctgc ggcggcggcg gcggcgggcg cgggcccgga gatggtccgc
      181 gggcaggtgt tcgacgtggg gccgcgctac accaacctct cgtacatcgg cgagggcgcc
      241 tacggcatgg tgtgctctgc ttatgataat gtcaacaaag ttcgagtagc tatcaagaaa
      301 atcagcccct ttgagcacca gacctactgc cagagaaccc tgagggagat aaaaatctta
      361 ctgcgcttca gacatgagaa catcattgga atcaatgaca ttattcgagc accaaccatc
      421 gagcaaatga aagatgtata tatagtacag gacctcatgg aaacagatct ttacaagctc
      481 ttgaagacac aacacctcag caatgaccat atctgctatt ttctctacca gatcctcaga
      541 gggttaaaat atatccattc agctaacgtt ctgcaccgtg acctcaagcc ttccaacctg
      601 ctgctcaaca ccacctgtga tctcaagatc tgtgactttg gcctggcccg tgttgcagat
      661 ccagaccatg atcacacagg gttcctgaca gaatatgtgg ccacacgttg gtacagggct
      721 ccagaaatta tgttgaattc caagggctac accaagtcca ttgatatttg gtctgtaggc
      781 tgcattctgg cagaaatgct ttctaacagg cccatctttc cagggaagca ttatcttgac
      841 cagctgaacc acattttggg tattcttgga tccccatcac aagaagacct gaattgtata
      901 ataaatttaa aagctaggaa ctatttgctt tctcttccac acaaaaataa ggtgccatgg
      961 aacaggctgt tcccaaatgc tgactccaaa gctctggact tattggacaa aatgttgaca
     1021 ttcaacccac acaagaggat tgaagtagaa caggctctgg cccac
//
