LOCUS       sequence-87679-e         822 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of alpha NTD fused via three alanines to E. coli RNAP 
            subunit beta, aa 831-1057 (beta-flap). Can be cut with Not1 and 
            BamHI to replace beta-flap with a moiety of interest..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Dove SL, Joung JK, Hochschild A
  TITLE     Activation of prokaryotic transcription through arbitrary 
            protein-protein contacts.
  JOURNAL   Nature. 1997 Apr 10;386(6625):627-30.
  PUBMED    9121589
REFERENCE   2  (bases 1 to 822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature."; 
            date: "1997-04-10"; volume: "386(6625)"; pages: "627-30"
COMMENT     Sequence Label: pBR-alpha-beta flap (831-1057)
FEATURES             Location/Qualifiers
     source          1..822
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 cacgatgcgt ccggcgtaga ggatccttat ttaaccgcca gatatacctt aacaatcttc
       61 agcacgcccg gtgccagatc gtcgccctgg gtgattttgc ggcgtttcgc ttcgagtttc
      121 ttctcgaact cgtgtttcag ttcgtcatac tgctcagcca gctgttccag ctgattttgt
      181 ttctcttcgt ctgtcaggcc cagctccagc cagcgatcgc gcggcagttt gtcgagcttc
      241 tcagcttcaa cgccaccggc taccagcaca gcacggatac ggctgaacag acccgcttcg
      301 aggatctgca gttcttcaga caggtctttc ttcgcctgtt tgagctgcat ttcttcgatt
      361 tccagcgcac gtttgtcttt ttctacgcca tcgcgagtaa agacctgaac gtcgataacc
      421 gtaccggata caccgtttgg tacgcgcaga gaagagtctt taacgtcaga ggctttctca
      481 ccgaagatcg cacgcagcag tttttcttct ggggtcagct gagtttcacc tttcggcgtt
      541 accttaccaa ccagaatgtc gccaccggtc acttccgcac caatgtaaac gataccggat
      601 tcatccagtt tggagagcgc agcttcaccc acgttcggga tgtcagcggt gatctcttcc
      661 ggacccagct tggtgtcacg ggacacacac gccagttcct gaatgtggat tgcggccgcc
      721 tctggtttct cttctttcac ttcaggctga cgtacatcac gtaagtcaac gaaagcttcc
      781 agttgttcag ccagaatggt tgccgcacga cgaatcgcct ct
//
