LOCUS       sequence-87680-e         859 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PlacUV5-directed synthesis of the lambda-CI protein; used as 
            two-hybrid control. For cloning purposes, see Addgene plasmid 53733.
            .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Dove SL, Joung JK, Hochschild A
  TITLE     Activation of prokaryotic transcription through arbitrary 
            protein-protein contacts.
  JOURNAL   Nature. 1997 Apr 10;386(6625):627-30.
  PUBMED    9121589
REFERENCE   2  (bases 1 to 859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature."; 
            date: "1997-04-10"; volume: "386(6625)"; pages: "627-30"
COMMENT     Sequence Label: pAC-lambda-CI
FEATURES             Location/Qualifiers
     source          1..859
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(138..143,144..161,162..168)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    176..192
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     181..203
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 aatatttcta gatttcagtg caatttatct cttcaaatgt agcacctgaa gtcagcccca
       61 tacgatataa gttgtaattc tcatgtttga cagcttatca tcgataagct aattctcact
      121 cattaggcac cccaggcttt acactttatg cttccggctc gtataatgtg tggaattgtg
      181 agcggataac aatttcacac aggaaacagc gtatgagcac aaaaaagaaa ccattaacac
      241 aagagcagct tgaggacgca cgtcgcctta aagcaattta tgaaaaaaag aaaaatgaac
      301 ttggcttatc ccaggaatct gtcgcagaca agatggggat ggggcagtca ggcgttggtg
      361 ctttatttaa tggcatcaat gcattaaatg cttataacgc cgcattgctt gcaaaaattc
      421 tcaaagttag cgttgaagaa tttagccctt caatcgccag agaaatctac gagatgtatg
      481 aagcggttag tatgcagccg tcacttagaa gtgagtatga gtaccctgtt ttttctcatg
      541 ttcaggcagg gatgttctca cctgagctta gaacctttac caaaggtgat gcggagagat
      601 gggtaagcac aaccaaaaaa gccagtgatt ctgcattctg gcttgaggtt gaaggtaatt
      661 ccatgaccgc accaacaggc tccaagccaa gctttcctga cggaatgtta attctcgttg
      721 accctgagca ggctgttgag ccaggtgatt tctgcatagc cagacttggg ggtgatgagt
      781 ttaccttcaa gaaactgatc agggatagcg gtcaggtgtt tttacaacca ctaaacccac
      841 agtacccaat gatcccatg
//
