LOCUS       sequence-87704-e         686 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of SOX4 after doxycycline administration.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 686)
  AUTHORS   Doulatov S, Vo LT, Chou SS, Kim PG, Arora N, Li H, Hadland BK, 
            Bernstein ID, Collins JJ, Zon LI, Daley GQ
  TITLE     Induction of multipotential hematopoietic progenitors from human 
            pluripotent stem cells via respecification of lineage-restricted 
            precursors.
  JOURNAL   Cell Stem Cell. 2013 Oct 3;13(4):459-70. doi: 
            10.1016/j.stem.2013.09.002.
  PUBMED    24094326
REFERENCE   2  (bases 1 to 686)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2013.09"; journalName: "Cell Stem Cell"; date: 
            "2013-10-3- 3"; volume: "13"; issue: "4"; pages: "459-70"
COMMENT     Sequence Label: pINDUCER21-SOX4
FEATURES             Location/Qualifiers
     source          1..686
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(147..149,150..152,153..665)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     complement(192..213)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(453..472)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt
       61 cgaggttaaa aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa
      121 cacgatgata agcttaccgg tccaccatgg tgagcaaggg cgaggagctg ttcaccgggg
      181 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
      241 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
      301 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct
      361 tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
      421 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
      481 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
      541 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct
      601 atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca
      661 tcgaggacgg cagcgtgcag ctcgcc
//
