LOCUS       sequence-87709-e         868 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of lambda-CI fused via three alanines to E. coli RNAP 
            beta subunit, aa 831-1057 (beta-flap). Can be cut with Not1 and 
            BamHI to replace beta-flap with a moiety of interest..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Dove SL, Joung JK, Hochschild A
  TITLE     Activation of prokaryotic transcription through arbitrary 
            protein-protein contacts.
  JOURNAL   Nature. 1997 Apr 10;386(6625):627-30.
  PUBMED    9121589
REFERENCE   2  (bases 1 to 868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature."; 
            date: "1997-04-10"; volume: "386(6625)"; pages: "627-30"
COMMENT     Sequence Label: pAC-lambda-CI-beta-flap (831-1057)
FEATURES             Location/Qualifiers
     source          1..868
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gggtagccag cagcatcctg cgatgcagat ctgtaaggta aggatcctta tttaaccgcc
       61 agatatacct taacaatctt cagcacgccc ggtgccagat cgtcgccctg ggtgattttg
      121 cggcgtttcg cttcgagttt cttctcgaac tcgtgtttca gttcgtcata ctgctcagcc
      181 agctgttcca gctgattttg tttctcttcg tctgtcaggc ccagctccag ccagcgatcg
      241 cgcggcagtt tgtcgagctt ctcagcttca acgccaccgg ctaccagcac agcacggata
      301 cggctgaaca gacccgcttc gaggatctgc agttcttcag acaggtcttt cttcgcctgt
      361 ttgagctgca tttcttcgat ttccagcgca cgtttgtctt tttctacgcc atcgcgagta
      421 aagacctgaa cgtcgataac cgtaccggat acaccgtttg gtacgcgcag agaagagtct
      481 ttaacgtcag aggctttctc accgaagatc gcacgcagca gtttttcttc tggggtcagc
      541 tgagtttcac ctttcggcgt taccttacca accagaatgt cgccaccggt cacttccgca
      601 ccaatgtaaa cgataccgga ttcatccagt ttggagagcg cagcttcacc cacgttcggg
      661 atgtcagcgg tgatctcttc cggacccagc ttggtgtcac gggacacaca cgccagttcc
      721 tgaatgtgga ttgcggccgc gccaaacgtc tcttcaggcc actgactagc gataactttc
      781 cccacaacgg aacaactctc attgcatggg atcattgggt actgtgggtt tagtggttgt
      841 aaaaacacct gaccgctatc cctgatca
//
