LOCUS       sequence-87760-e         662 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 662)
  AUTHORS   Binder A, Lambert J, Morbitzer R, Popp C, Ott T, Lahaye T, Parniske 
            M
  TITLE     A modular plasmid assembly kit for multigene expression, gene 
            silencing and silencing rescue in plants.
  JOURNAL   PLoS One. 2014 Feb 13;9(2):e88218. doi: 
            10.1371/journal.pone.0088218. eCollection 2014.
  PUBMED    24551083
REFERENCE   2  (bases 1 to 662)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-02-13"; volume: "9(2):e88218. doi"; pages: " 
            10.1371/journal.pone.0088218. eCollection 2014"
COMMENT     Sequence Label: LIIIbeta R C-D
FEATURES             Location/Qualifiers
     source          1..662
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(86..391)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(188..207)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 aacactgata gtttaaacca gaggtctcac ctttacgaag tcttcgtcga cctgcagact
       61 ggctgtgtat aagggagcct gacatttata ttccccagaa catcaggtta atggcgtttt
      121 tgatgtcatt ttcgcggtgg ctgagatcag ccacttcttc cccgataacg gaaaccggca
      181 cactggccat atcggtggtc atcatgcgcc agctttcatc cccgatatgc accaccgggt
      241 aaagttcacg ggggacttta tctgacagca gacgtgcact ggccaggggg atcaccatcc
      301 gtcgcccggg cgtgtcaata atatcactct gtacatccac aaacagacga taacggctct
      361 ctcttttata ggtgtaaacc ttaaactgca tttcaccagc ccctgttctc gtcagcaaaa
      421 gagccgttca tttcaataaa ccgggcgacc tcagccatcc cttcctgatt ttccgctttc
      481 cagcgttcgg cacgcagacg acgggcttca ttctgcatgg ttgtgcttac cagaccggag
      541 atattgacat catatatgcc ttgagcaact gatagctgtc gctgtcaact gtcactgtaa
      601 tacgctgctt catagcatac ctctttttga catacttcgg gtatacatat cagtatatat
      661 tc
//
