LOCUS       sequence-88298-e        1714 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus, Excitation: 400 / 504, Emission: 515 / 517.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1714)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 1714)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mPA-GFP-PCNA-19-SV40NLS-4
FEATURES             Location/Qualifiers
     source          1..1714
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      18..27
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             30..50
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             54..74
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             78..98
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      105..114
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(111..113,114..116,117..827)
                     /codon_start=1
                     /product="photoactivatable GFP variant (Patterson et al., 
                     2002)"
                     /label=PA-GFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(156..177)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(417..436)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     764..785
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 agacgtcaga tccgctagcc accatggatc caaaaaagaa gagaaaggta gatccaaaaa
       61 agaagagaaa ggtagatcca aaaaagaaga gaaaggtacc ggtcgccacc atggtgagca
      121 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
      181 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
      241 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
      301 ccttcagcta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact
      361 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
      421 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
      481 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
      541 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg
      601 ccaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
      661 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagcc
      721 accagtccaa gctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
      781 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtcc ggagaagggc
      841 aagggcaagg gcaagggccg ggccgcggct acgcgtatcg atccatgttc gaggcgcgcc
      901 tggtccaggg ctccatcctc aagaaggtgt tggaggcact caaggacctc atcaacgagg
      961 cctgctggga tattagctcc agcggtgtaa acctgcagag catggactcg tcccacgtct
     1021 ctttggtgca gctcaccctg cggtctgagg gcttcgacac ctaccgctgc gaccgcaacc
     1081 tggccatggg cgtgaacctc accagtatgt ccaaaatact aaaatgcgcc ggcaatgaag
     1141 atatcattac actaagggcc gaagataacg cggatacctt ggcgctagta tttgaagcac
     1201 caaaccagga gaaagtttca gactatgaaa tgaagttgat ggatttagat gttgaacaac
     1261 ttggaattcc agaacaggag tacagctgtg tagtaaagat gccttctggt gaatttgcac
     1321 gtatatgccg agatctcagc catattggag atgctgttgt aatttcctgt gcaaaagacg
     1381 gagtgaaatt ttctgcaagt ggagaacttg gaaatggaaa cattaaattg tcacagacaa
     1441 gtaatgtcga taaagaggag gaagctgtta ccatagagat gaatgaacca gttcaactaa
     1501 cttttgcact gaggtacctg aacttcttta caaaagccac tccactctct tcaacggtga
     1561 cactcagtat gtctgcagat gtaccccttg ttgtagagta taaaattgcg gatatgggac
     1621 acttaaaata ctacttggct cccaagatcg aggatgaaga aggatcttag ggatccaccg
     1681 gatctagata actgatcata atcagccata ccac
//
