LOCUS       sequence-88310-e        1961 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 400 / 504, Emission: 515 / 517.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1961)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 1961)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PA-mCherry1-Actin-C-18
FEATURES             Location/Qualifiers
     source          1..1961
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      22..31
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             28..735
                     /codon_start=1
                     /product="photoactivatable monomeric derivative of DsRed 
                     fluorescent protein (Subach et al., 2009)"
                     /label=PAmCherry1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHVFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFTWDILSPQFMYGSNAYVKHPADIPDYFKLSFPEGFKWERVMKF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEALSERMYPEDGALK
                     GEVKPRVKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNRKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(173..191)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 acgtcagatc cgctagcggt cgccaccatg gtgagcaagg gcgaggagga taacatggcc
       61 atcattaagg agttcatgcg cttcaaggtg cacatggagg ggtccgtgaa cggccacgtg
      121 ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg
      181 aaggtgacca agggcggccc cctgcccttc acctgggaca tcctgagccc tcagttcatg
      241 tacggctcca atgcctacgt gaagcacccc gccgacatcc ccgactactt taagctgtcc
      301 ttccccgagg gcttcaagtg ggagcgcgtg atgaaattcg aggacggcgg cgtggtgacc
      361 gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc
      421 accaacttcc cctccgacgg ccccgtgatg cagaagaaga ccatgggctg ggaggccctc
      481 tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg aggtcaagcc cagagtgaag
      541 ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc
      601 gtgcagctgc ccggcgccta caacgtcaac cgcaagctgg acatcaccag ccacaacgag
      661 gactacacca tcgtggagca gtacgagaga gccgagggcc gccactccac cggcggcatg
      721 gacgagctgt acaagtccgg actcagatct ggcagcggtg gaggcagcgc atccggcgga
      781 agcggaagcg atgatgatat cgccgcgctc gtcgtcgaca acggctccgg catgtgcaag
      841 gccggcttcg cgggcgacga tgccccccgg gccgtcttcc cctccatcgt ggggcgcccc
      901 aggcaccagg gcgtgatggt gggcatgggt cagaaggatt cctatgtggg cgacgaggcc
      961 cagagcaaga gaggcatcct caccctgaag taccccatcg agcacggcat cgtcaccaac
     1021 tgggacgaca tggagaaaat ctggcaccac accttctaca atgagctgcg tgtggctccc
     1081 gaggagcacc ccgtgctgct gaccgaggcc cccctgaacc ccaaggccaa ccgcgagaag
     1141 atgacccaga tcatgtttga gaccttcaac accccagcca tgtacgttgc tatccaggct
     1201 gtgctatccc tgtacgcctc tggccgtacc actggcatcg tgatggactc cggtgacggg
     1261 gtcacccaca ctgtgcccat ctacgagggg tatgccctcc cccatgccat cctgcgtctg
     1321 gacctggctg gccgggacct gactgactac ctcatgaaga tcctcaccga gcgcggctac
     1381 agcttcacca ccacggccga gcgggaaatc gtgcgtgaca ttaaggagaa gctgtgctac
     1441 gtcgccctgg acttcgagca agagatggcc acggctgctt ccagctcctc cctggagaag
     1501 agctacgagc tgcctgacgg ccaggtcatc accattggca atgagcggtt ccgctgccct
     1561 gaggcactct tccagccttc cttcctgggc atggagtcct gtggcatcca cgaaactacc
     1621 ttcaactcca tcatgaagtg tgacgtggac atccgcaaag acctgtacgc caacacagtg
     1681 ctgtctggcg gcaccaccat gtaccctggc attgccgaca ggatgcagaa ggagatcact
     1741 gccctggcac ccagcacaat gaagatcaag atcattgctc ctcctgagcg caagtactcc
     1801 gtgtggatcg gcggctccat cctggcctcg ctgtccacct tccagcagat gtggatcagc
     1861 aagcaggagt atgacgagtc cggcccctcc atcgtccacc gcaaatgctt ctaggatcca
     1921 ccggatctag ataactgatc ataatcagcc ataccacatt t
//
