LOCUS       sequence-88323-e        2288 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytoskeleton, Excitation: 400 / 504, Emission: 515 / 
            517.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2288)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 2288)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PA-mCherry1-Fascin-C-10
FEATURES             Location/Qualifiers
     source          1..2288
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             25..732
                     /codon_start=1
                     /product="photoactivatable monomeric derivative of DsRed 
                     fluorescent protein (Subach et al., 2009)"
                     /label=PAmCherry1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHVFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFTWDILSPQFMYGSNAYVKHPADIPDYFKLSFPEGFKWERVMKF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEALSERMYPEDGALK
                     GEVKPRVKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNRKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(170..188)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 tccgctagcg ctaccggtcg ccacatggtg agcaagggcg aggaggataa catggccatc
       61 attaaggagt tcatgcgctt caaggtgcac atggaggggt ccgtgaacgg ccacgtgttc
      121 gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag
      181 gtgaccaagg gcggccccct gcccttcacc tgggacatcc tgagccctca gttcatgtac
      241 ggctccaatg cctacgtgaa gcaccccgcc gacatccccg actactttaa gctgtccttc
      301 cccgagggct tcaagtggga gcgcgtgatg aaattcgagg acggcggcgt ggtgaccgtg
      361 acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct gcgcggcacc
      421 aacttcccct ccgacggccc cgtgatgcag aagaagacca tgggctggga ggccctctcc
      481 gagcggatgt accccgagga cggcgccctg aagggcgagg tcaagcccag agtgaagctg
      541 aaggacggcg gccactacga cgctgaggtc aagaccacct acaaggccaa gaagcccgtg
      601 cagctgcccg gcgcctacaa cgtcaaccgc aagctggaca tcaccagcca caacgaggac
      661 tacaccatcg tggagcagta cgagagagcc gagggccgcc actccaccgg cggcatggac
      721 gagctgtaca agtccggagc tggtagtggt ggaagcgcag gaaccgccaa cggcacagcc
      781 gaggcggtgc agatccagtt cggcctcatc aactgcggca acaagtacct gacggccgag
      841 gcgttcgggt tcaaggtgaa cgcgtccgcc agcagcctga agaagaagca gatctggacg
      901 ctggagcagc cccctgacga ggcgggcagc gcggccgtgt gcctgcgcag ccacctgggc
      961 cgctacctgg cggcggacaa ggacggcaac gtgacctgcg agcgcgaggt gcccggtccc
     1021 gactgccgtt tcctcatcgt ggcgcacgac gacggtcgct ggtcgctgca gtccgaggcg
     1081 caccggcgct acttcggcgg caccgaggac cgcctgtcct gcttcgcgca gacggtgtcc
     1141 cccgccgaga agtggagcgt gcacatcgcc atgcaccctc aggtcaacat ctacagcgtc
     1201 acccgtaagc gctacgcgca cctgagcgcg cggccggccg acgagatcgc cgtggaccgc
     1261 gacgtgccct ggggcgtcga ctcgctcatc accctcgcct tccaggacca gcgctacagc
     1321 gtgcagaccg ccgaccaccg cttcctgcgc cacgacgggc gcctggtggc gcgccccgag
     1381 ccggccactg gctacacgct ggagttccgc tccggcaagg tggccttccg cgactgcgag
     1441 ggccgttacc tggcgccgtc ggggcccagc ggcacgctca aggcgggcaa ggccaccaag
     1501 gtgggcaagg acgagctctt tgctctggag cagagctgcg cccaggtcgt gctgcaggcg
     1561 gccaacgaga ggaacgtgtc cacgcgccag ggtatggacc tgtctgccaa tcaggacgag
     1621 gagaccgacc aggagacctt ccagctggag atcgaccgcg acaccaaaaa gtgtgccttc
     1681 cgtacccaca cgggcaagta ctggacgctg acggccaccg ggggcgtgca gtccaccgcc
     1741 tccagcaaga atgccagctg ctactttgac atcgagtggc gtgaccggcg catcacactg
     1801 agggcgtcca atggcaagtt tgtgacctcc aagaagaatg ggcagctggc cgcctcggtg
     1861 gagacggcag gggactcaga gctcttcctc atgaagctca tcaaccgccc catcatcgtg
     1921 ttccgcgggg agcatggctt catcggctgc cgcaaggtca cgggcaccct ggacgccaac
     1981 cgctccagct atgacgtctt ccagctggag ttcaacgatg gcgcctacaa catcaaagac
     2041 tccacaggca aatactggac ggtgggcagt gactccgtgg tcaccagcag cggcgacact
     2101 cctgtggact tcttcttcga gttctgcgac tataacaagg tggccatcaa ggtgggcggg
     2161 cgctacctga agggcgacca cgcaggcgtc ctgaaggcct cggcggaaac cgtggacccc
     2221 gcctcgctct gggagtacta gggatccacc ggatctagat aactgatcat aatcagccat
     2281 accacatt
//
