LOCUS       sequence-88339-e        1407 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Mitochondria, Excitation: 400 / 504, Emission: 515 / 
            517.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1407)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 1407)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PA-mCherry1-PMP-N-10
FEATURES             Location/Qualifiers
     source          1..1407
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      36..45
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      651..660
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             657..1367
                     /codon_start=1
                     /product="photoactivatable monomeric derivative of DsRed 
                     fluorescent protein (Subach et al., 2009)"
                     /label=PAmCherry1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHVFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFTWDILSPQFMYGSNAYVKHPADIPDYFKLSFPEGFKWERVMKF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEALSERMYPEDGALK
                     GEVKPRVKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNRKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(802..820)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 acgtcagatc cgctagcgct accggactca gatctgccac catggcgccg gccgcgtcca
       61 ggctgcgggc cgaagccggg ctcggggcgc tgccgcggcg ggcgctcgcc cagtacttgc
      121 tcttcctgcg gctctacccg gtgctcacca aggcggccac cagtggcatt ttgtcagcac
      181 ttgggaactt cctggcccag atgattgaga agaagcggaa aaaagaaaac tctagaagtc
      241 tggatgtcgg tgggcctctg agatatgccg tttacgggtt cttcttcaca gggccgctga
      301 gtcacttctt ctacttcttc atggaacatt ggatccctcc tgaggtcccc ctggcagggc
      361 tcaggaggct tctcctggac cgcctcgtct ttgcaccggc cttcctcatg ttgttcttcc
      421 tcatcatgaa ctttctggag gggaaagacg cctcagcctt cgccgccaag atgagggggg
      481 gcttctggcc ggcgctgagg atgaactggc gggtgtggac gccactacag ttcatcaaca
      541 tcaactacgt ccctctgaag ttccgggtgc tcttcgccaa cctggcagct ctgttctggt
      601 atgcctacct ggcctccttg gggaagagcg gctccggtag cggaccggtc gccaccatgg
      661 tgagcaaggg cgaggaggat aacatggcca tcattaagga gttcatgcgc ttcaaggtgc
      721 acatggaggg gtccgtgaac ggccacgtgt tcgagatcga gggcgagggc gagggccgcc
      781 cctacgaggg cacccagacc gccaagctga aggtgaccaa gggcggcccc ctgcccttca
      841 cctgggacat cctgagccct cagttcatgt acggctccaa tgcctacgtg aagcaccccg
      901 ccgacatccc cgactacttt aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga
      961 tgaaattcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg
     1021 agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtgatgc
     1081 agaagaagac catgggctgg gaggccctct ccgagcggat gtaccccgag gacggcgccc
     1141 tgaagggcga ggtcaagccc agagtgaagc tgaaggacgg cggccactac gacgctgagg
     1201 tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaacc
     1261 gcaagctgga catcaccagc cacaacgagg actacaccat cgtggagcag tacgagagag
     1321 ccgagggccg ccactccacc ggcggcatgg acgagctgta caagtaagcg gccgcgactc
     1381 tagatcataa tcagccatac cacattt
//
