LOCUS       sequence-88366-e        1048 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane, Excitation: 400 / 490, Emission: 468 / 511.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1048)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 1048)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PS-CFP2-Farnesyl-5
FEATURES             Location/Qualifiers
     source          1..1048
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        35..86
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     37..57
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             131..844
                     /codon_start=1
                     /product="photoswitchable cyan-to-green fluorescent 
                     protein"
                     /label=PS-CFP2
                     /note="mammalian codon-optimized"
                     /translation="MSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVATLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDDG
                     NYKTRAEVKFEGDTLVSRIELTGTDFKEDGNILGNKMEYNYNATNVYIVADKARNGIKV
                     NFKVRHNIKDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYLE
                     FVTAAAITHGMDELYK"
ORIGIN
        1 actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac
       61 ggtgggaggt ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcgctacc
      121 ggtcgccacc atgagcaagg gcgccgagct gttcaccggc atcgtgccca tcctgatcga
      181 gctgaatggc gatgtgaatg gccacaagtt cagcgtgagc ggcgagggcg agggcgatgc
      241 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ctgtgccctg
      301 gcccaccctg gtggccaccc tgagctacgg cgtgcagtgc ttctcacgct accccgatca
      361 catgaagcag cacgacttct tcaagagcgc catgcctgag ggctacatcc aggagcgcac
      421 catcttcttc gaggatgacg gcaactacaa gacgcgcgcc gaggtgaagt tcgagggcga
      481 taccctggtg agtcgcatcg agctgaccgg cactgatttc aaggaggatg gcaacatcct
      541 gggcaataag atggagtaca actacaacgc caccaatgtg tacatcgtgg ccgacaaggc
      601 caggaatggc atcaaggtga acttcaaggt ccgccacaac atcaaggatg gcagcgtgca
      661 gctggccgac cactaccagc agaatacccc catcggcgat ggccctgtgc tgctgcccga
      721 taaccactac ctgtccaccc agagcgccct gtccaaggac cccaacgaga agcgcgatca
      781 catgatctac ctcgagttcg tgaccgccgc cgccatcacc cacggcatgg atgagctgta
      841 caagtccgga ctcagatcta agctgaaccc tcctgatgag agtggccccg gctgcatgag
      901 ctgcaagtgt gtgctctcct gagaattctg cagtcgacgg taccgcgggc ccgggatcca
      961 ccggatctag ataactgatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt
     1021 taaaaaacct cccacacctc cccctgaa
//
