LOCUS       sequence-88402-e        2546 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endoplasmic Reticulum, Excitation: 503, Emission: 518.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2546)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 2546)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Dronpa-Calnexin-N-14
FEATURES             Location/Qualifiers
     source          1..2546
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1828..1837
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1834..2511
                     /codon_start=1
                     /product="monomeric photoswitchable Dronpa-Green1 
                     fluorescent protein"
                     /label=Dronpa-Green1
                     /translation="MVSVIKPDMKIKLRMEGAVNGHPFAIEGVGLGKPFEGKQSMDLKV
                     KEGGPLPFAYDILTTVFCYGNRVFAKYPENIVDYFKQSFPEGYSWERSMNYEDGGICNA
                     TNDITLDGDCYIYEIRFDGVNFPANGPVMQKRTVKWEPSTEKLYVRDGVLKGDVNMALS
                     LEGGGHYRCDFKTTYKAKKVVQLPDYHFVDHHIEIKSHDKDYSNVNLHEHAEAHSELPR
                     QAK"
ORIGIN
        1 gatctcgaga ccgccatgga agggaagtgg ttgctgtgta tgttactggt gcttggaact
       61 gctattgttg aggctcatga tggacatgat gatgatgtga ttgatattga ggatgacctt
      121 gacgatgtca ttgaagaggt agaagactca aaaccagata ccactgctcc tccttcatct
      181 cccaaggtta cttacaaagc tccagttcca acaggggaag tatattttgc tgattctttt
      241 gacagaggaa ctctgtcagg gtggatttta tccaaagcca agaaagacga taccgatgat
      301 gaaattgcca aatatgatgg aaagtgggag gtagaggaaa tgaaggagtc aaagcttcca
      361 ggtgataaag gacttgtgtt gatgtctcgg gccaagcatc atgccatctc tgctaaactg
      421 aacaagccct tcctgtttga caccaagcct ctcattgttc agtatgaggt taatttccaa
      481 aatggaatag aatgtggtgg tgcctatgtg aaactgcttt ctaaaacacc agaactcaac
      541 ctggatcagt tccatgacaa gaccccttat acgattatgt ttggtccaga taaatgtgga
      601 gaggactata aactgcactt catcttccga cacaaaaacc ccaaaacggg tatctatgaa
      661 gaaaaacatg ctaagaggcc agatgcagat ctgaagacct attttactga taagaaaaca
      721 catctttaca cactaatctt gaatccagat aatagttttg aaatactggt tgaccaatct
      781 gtggtgaata gtggaaatct gctcaatgac atgactcctc ctgtaaatcc ttcacgtgaa
      841 attgaggacc cagaagaccg gaagcccgag gattgggatg aaagaccaaa aatcccagat
      901 ccagaagctg tcaagccaga tgactgggat gaagatgccc ctgctaagat tccagatgaa
      961 gaggccacaa aacccgaagg ctggttagat gatgagcctg agtacgtacc tgatccagac
     1021 gcagagaaac ctgaggattg ggatgaagac atggatggag aatgggaggc tcctcagatt
     1081 gccaacccta gatgtgagtc agctcctgga tgtggtgtct ggcagcgacc tgtgattgac
     1141 aaccccaatt ataaaggcaa atggaagcct cctatgattg acaatcccag ttaccaggga
     1201 atctggaaac ccaggaaaat accaaatcca gatttctttg aagatctgga acctttcaga
     1261 atgactcctt ttagtgctat tggtttggag ctgtggtcca tgacctctga catttttttt
     1321 gacaacttta tcatttgtgc tgatcgaaga atagttgatg attgggccaa tgatggatgg
     1381 ggcctgaaga aagctgctga tggggctgct gagccaggcg ttgtggggca gatgaacgag
     1441 gcagctgaag agcgcccgtg gctgtgggta gtctatattc taactgtagc ccttcctgtg
     1501 ttcctggtta tcctcttctg ctgttctgga aagaaacaga ccagtggtat ggagtataag
     1561 aaaactgatg cacctcaacc ggatgtgaag gaagaggaag aagagaagga agaggaaaag
     1621 gacaagggag atgaggagga ggaaggagaa gagaaacttg aagagaaaca gaaaagtgat
     1681 gctgaagaag atggtggcac tgtcagtcaa gaggaggaag acagaaaacc taaagcagag
     1741 gaggatgaaa ttttgaacag atcaccaaga aacagaaagc cacgaagaga ggggggtgcg
     1801 tcaggcggca gcggatctgg accggtcgcc accatggtga gtgtgattaa accagacatg
     1861 aagatcaagc tgcgtatgga aggcgctgta aatggacacc cgttcgcgat tgaaggagtt
     1921 ggccttggga agcctttcga gggaaaacag agtatggacc ttaaagtcaa agaaggcgga
     1981 cctctgcctt tcgcctatga catcttgaca actgtgttct gttacggcaa cagggtattc
     2041 gccaaatacc cagaaaatat agtagactat ttcaagcagt cgtttcctga gggctactct
     2101 tgggaacgaa gcatgaatta cgaagacggg ggcatttgta acgcgacaaa cgacataacc
     2161 ctggatggtg actgttatat ctatgaaatt cgatttgatg gtgtgaactt tcctgccaat
     2221 ggtccagtta tgcagaagag gactgtgaaa tgggagccat ccactgagaa attgtatgtg
     2281 cgtgatggag tgctgaaggg tgatgttaac atggctctgt cgcttgaagg aggtggccat
     2341 taccgatgtg acttcaaaac tacttataaa gctaagaagg ttgtccagtt gccagactat
     2401 cactttgtgg accaccacat tgagattaaa agccacgaca aagattacag taatgttaat
     2461 ctgcatgagc acgccgaagc gcattctgag ctgccgaggc aggccaagta atgataggcg
     2521 gccgcgactc tagatcataa tcagcc
//
