LOCUS       sequence-88435-e        8520 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 503, Emission: 518.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8520)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 8520)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Dronpa-Talin-18
FEATURES             Location/Qualifiers
     source          1..8520
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      26..35
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             32..706
                     /codon_start=1
                     /product="monomeric photoswitchable Dronpa-Green1 
                     fluorescent protein"
                     /label=Dronpa-Green1
                     /translation="MVSVIKPDMKIKLRMEGAVNGHPFAIEGVGLGKPFEGKQSMDLKV
                     KEGGPLPFAYDILTTVFCYGNRVFAKYPENIVDYFKQSFPEGYSWERSMNYEDGGICNA
                     TNDITLDGDCYIYEIRFDGVNFPANGPVMQKRTVKWEPSTEKLYVRDGVLKGDVNMALS
                     LEGGGHYRCDFKTTYKAKKVVQLPDYHFVDHHIEIKSHDKDYSNVNLHEHAEAHSELPR
                     QAK"
     regulatory      755..764
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gtcagatccg ctagcgctac cggtcgccac catggtgagt gtgattaaac cagacatgaa
       61 gatcaagctg cgtatggaag gcgctgtaaa tggacacccg ttcgcgattg aaggagttgg
      121 ccttgggaag cctttcgagg gaaaacagag tatggacctt aaagtcaaag aaggcggacc
      181 tctgcctttc gcctatgaca tcttgacaac tgtgttctgt tacggcaaca gggtattcgc
      241 caaataccca gaaaatatag tagactattt caagcagtcg tttcctgagg gctactcttg
      301 ggaacgaagc atgaattacg aagacggggg catttgtaac gcgacaaacg acataaccct
      361 ggatggtgac tgttatatct atgaaattcg atttgatggt gtgaactttc ctgccaatgg
      421 tccagttatg cagaagagga ctgtgaaatg ggagccatcc actgagaaat tgtatgtgcg
      481 tgatggagtg ctgaagggtg atgttaacat ggctctgtcg cttgaaggag gtggccatta
      541 ccgatgtgac ttcaaaacta cttataaagc taagaaggtt gtccagttgc cagactatca
      601 ctttgtggac caccacattg agattaaaag ccacgacaaa gattacagta atgttaatct
      661 gcatgagcac gccgaagcgc attctgagct gccgaggcag gccaagtccg gactcagatc
      721 tcgagctcaa gctagcttcg aattcgcaga agctgccacc atggttgcgc tttcgctgaa
      781 gattagcatt gggaatgtgg tgaagacgat gcaatttgag ccatctacca tggtgtacga
      841 cgcctgccgc atgattcgtg agcggatccc agaggccctg gctggccctc ccaacgactt
      901 tgggctcttt ctgtcagatg atgaccccaa aaaaggcatc tggctagagg ctgggaaagc
      961 tttggactac tacatgctcc gaaatgggga caccatggag tacagaaaaa agcagagacc
     1021 cctgaagata cggatgttag acggaacggt gaagactatc atggtggatg actccaagac
     1081 cgtcacagac atgctcatga ccatttgtgc ccgaattggt atcaccaacc atgatgagta
     1141 ttcactggtt cgagagctga tggaagaaaa gaaagatgag gggacaggga ccctgagaaa
     1201 agacaagacc ctactgcgag atgaaaagaa gatggagaag ctaaagcaga agttgcacac
     1261 agacgatgag ttgaactggc tggaccatgg gcggacactg agggaacagg gagtggaaga
     1321 gcatgagacg ttgctgctgc ggagaaagtt cttctactca gaccagaatg tggattcccg
     1381 agaccctgta cagctgaacc ttctctatgt gcaggcacga gatgacatcc tgaatggctc
     1441 ccatcctgtc tcctttgaca aggcctgtga atttgcaggc ttccagtgcc agatccagtt
     1501 tggacctcac aatgaacaga agcacaaggc tggcttcctt gacctgaagg acttcctgcc
     1561 caaggagtat gtgaagcaga agggagagcg taagatcttt caggcacaca agaattgtgg
     1621 gcagatgagt gaaattgagg ccaaggtacg ctacgtgaag ctggcccgtt ccctcaagac
     1681 ttacggtgtc tccttcttcc tagtcaagga aaagatgaag gggaagaata aactggtgcc
     1741 caggctgttg ggcatcacta aggagtgtgt gatgcgtgtg gatgagaaga ccaaggaggt
     1801 gattcaggaa tggagcctca ccaacatcaa acgctgggct gcctctccca agagctttac
     1861 tctggacttt ggagactatc aggatggcta ctactcagta cagacgacag aaggcgagca
     1921 gatcgcacag ctcattgctg gctacataga tatcatcctt aagaagaaaa aaagcaagga
     1981 ccattttggg ctggagggag atgaagagtc tactatgttg gaggactcag tgtctcccaa
     2041 aaagtcaaca gtccttcagc agcagtacaa ccgagtgggg aaagtggagc acggctctgt
     2101 ggctctgcca gccatcatgc gctctggagc ctctggtcct gagaatttcc aggtggggag
     2161 catgccacct gcccagcagc agatcaccag tggccagatg caccgaggac acatgccacc
     2221 tctgacttca gcccagcagg cgctcactgg aaccattaac tccagcatgc aggctgtgca
     2281 ggctgcccag gccactctgg atgactttga gactctaccc cctcttggcc aggatgctgc
     2341 ctccaaagct tggcgtaaga acaagatgga tgaatcaaag catgagatcc actcccaggt
     2401 agatgccatc acagctggca ctgcctctgt ggtaaacctg acagcaggag atcctgcaga
     2461 gacagactat acagcagtgg gctgtgcagt caccaccatc tcttccaacc tgacggagat
     2521 gtcccgtggg gtgaagctat tggctgccct gctggaggat gaaggcggca atggccggcc
     2581 ccttctgcaa gcagcaaagg gccttgcagg ggctgtgtcg gaactgcttc gcagtgctca
     2641 gcccgccagc gctgagcctc gtcagaacct gctgcaggca gccgggaacg tgggccaggc
     2701 cagtggggag ctgttgcagc aaattgggga aagtgacact gacccccact tccaggatgt
     2761 tctaatgcag ctagcaaacg cagtggcaag tgctgcagcc gccctggtcc tcaaggccaa
     2821 gagtgtggcc cagcgcacag aggattccgg gcttcagacc caagtgattg ctgcagctac
     2881 gcaatgtgct ctgtccactt cccagctggt ggcctgtacc aaggtggtag cacctacaat
     2941 cagctcacct gtgtgccaag aacagctagt cgaggctggg cgactggtgg ccaaagctgt
     3001 ggagggctgt gtgtctgcct cgcaagcagc tacagaagat ggacagctgc ttcgaggggt
     3061 aggagcagca gccacggctg tcacccaggc cctcaatgag ctgctgcagc acgtgaaggc
     3121 ccacgccacc ggagctgggc ctgccggtcg gtatgaccaa gccactgata ccatcctcac
     3181 cgtcactgaa aacatcttca gctccatggg tgatgctggg gagatggtgc gccaggcccg
     3241 catcctggcc caagccacct cagacctggt caatgctatc aaggccgatg ctgaggggga
     3301 gagtgatctg gagaactcta gaaagctcct gagtgctgcc aagatcctcg ctgatgccac
     3361 cgccaagatg gtggaggcgg ccaagggagc agccgcccac cctgacagtg aggaacagca
     3421 gcagcgactg cgtgaagcag ctgaggggct tcgcatggcc accaatgcag ctgcgcagaa
     3481 cgccatcaag aagaagttgg tgcagcgcct ggagcatgca gccaagcaag ctgcagcctc
     3541 tgcaacacag accattgctg cagcccaaca tgcagcctct gcccccaagg cctctgcggg
     3601 cccccagccc ctactggtgc agagctgtaa ggccgtggca gagcagattc cgttgctggt
     3661 gcagggtgtc cgaggaagtc aagctcagcc tgacagccct agtgctcagc tcgccctcat
     3721 tgctgccagc cagagcttcc tgcagccagg tgggaagatg gtggcagcgg caaaagcctc
     3781 agtgccaaca attcaggacc aggcttcagc gatgcagctg agtcagtgcg caaagaacct
     3841 aggcactgcc ctggccgagc tgcgcactgc tgctcagaag gctcaggagg catgtggacc
     3901 tttggagatg gattctgcac tgagtgttgt acaaaatcta gagaaagacc tacaggaaat
     3961 aaaagcagca gctcgagatg gcaagcttaa gcccttacct ggggaaacaa tggagaagtg
     4021 tacccaagat cttggcaaca gcaccaaagc ggtgagctct gccatcgcca agctgctggg
     4081 ggagattgcc cagggcaacg agaactatgc aggtattgca gctcgggatg tagctggtgg
     4141 actaaggtca ctagcccagg ctgcgcgggg tgtggctgcg ctgacatcag atcctgcagt
     4201 gcaggccatt gtgcttgaca cagccagcga cgttctggac aaggccagca gcctcattga
     4261 agaggcaaaa aaagcatctg gacacccagg ggacccagaa agccagcaaa ggcttgctca
     4321 ggtagctaaa gcagtgaccc aagccctgaa ccgctgtgtc agctgcctgc ctggccaaag
     4381 agacgtggat aatgccctac gagccgttgg agatgccagc aagcgactcc tgagtgactt
     4441 gcttcctcca agcacgggga catttcaaga agcacagagc cgattgaatg aagctgcagc
     4501 tgggttaaat caggccgcca cagaactggt gcaagcctcc cgaggaactc ctcaggacct
     4561 ggctcgggcc tccggccgat ttggacagga cttcagcacc ttcctggaag ctggtgtgga
     4621 gatggccgga caggccccga gccaggagga ccgagcccag gtggtgtcca acctgaaggg
     4681 catatccatg tcttcaagca aacttctcct tgccgctaag gccttgtcca cagaccctgc
     4741 ttctcccaac ctcaagagtc agctggctgc ggctgcccgg gcagtgacgg acagcatcaa
     4801 ccagctcatc accatgtgca cccagcaggc acctggccag aaggagtgtg acaatgcact
     4861 tcggcagcta gagacagtcc gagaactcct ggagaatcca gtccagccca tcaacgacat
     4921 gtcctacttc ggttgcttgg acagtgtcat ggagaactcc aaggtcctag gtgaggccat
     4981 gactggcatc tcccaaaatg ccaagaatgg aaatctgccg gagtttggag atgccattgc
     5041 cacagcctcc aaggctctct gtggcttcac ggaggcagcc gcacaggcag catatctagt
     5101 tggtgtctct gaccccaaca gccaagctgg acagcaagga ctggtggaac ccacacagtt
     5161 tgcccgtgca aaccaggcaa ttcagatggc ctgtcagagt ctgggggagc ctggctgtac
     5221 ccaggcccag gtgttatctg cagccactat tgtagccaaa cacacatctg cattgtgtaa
     5281 cagctgtcgc ctggcttccg ctaggactgc caatcccact gccaagcgcc agtttgtaca
     5341 gtcagccaag gaggtggcca acagtacagc caatcttgtc aagaccatca aggcactaga
     5401 tggggacttc acagaagaga accgtgccca gtgccgagca gccacagccc ctctgctgga
     5461 agctgtggat aacctgagtg cctttgcctc caaccctgag ttctccagcg tccctgccca
     5521 gatcagccct gagggcagag cagccatgga gcccattgta atctctgcta agacaatgtt
     5581 ggagagtgct ggagggctca tccagacagc tcgggcgtta gcagtcaatc cccgagaccc
     5641 cccacgttgg tctgtgctag ctggccactc tcggactgtc tcagattcca tcaagaaact
     5701 tattacaagc atgagggaca aagccccagg gcagctggag tgtgagacag ccattgcggc
     5761 tctgaacagc tgcttgcggg acctagatca ggcttcgctt gccgctgtca gccagcagct
     5821 tgctccccgt gaaggaatct ctcaagaggc tttgcacacc cagatgctca ctgcagtgca
     5881 agaaatttct catctcattg agccgctggc cagcgctgct cgggctgaag cctcccagct
     5941 gggacacaag gtgtcccaaa tggcccagta ctttgaacca ctcaccctgg ctgcagtggg
     6001 tgctgcgtct aagaccctga gccacccgca gcagatggca cttctggacc agactaaaac
     6061 gttggcagag tctgccttgc agttgctata tactgccaag gaggctggtg gtaaccccaa
     6121 gcaagcagca cacacccagg aagccctgga ggaggctgtg cagatgatga cagaggccgt
     6181 agaagacctg acgacgaccc tcaacgaagc agccagtgct gcaggagtcg ttggcggcat
     6241 ggtggactct atcactcagg ccatcaacca gctagatgaa ggacctatgg gtgacccaga
     6301 aggctcattc gtagattacc agacaaccat ggtgaggaca gccaaggcca ttgctgtcac
     6361 tgttcaggag atggtaacca agtcaaacac cagccctgaa gagctaggcc ctcttgccaa
     6421 ccagctgacc agtgactatg gccgactggc ctcacaagcc aagcctgcag ctgtggctgc
     6481 tgaaaatgaa gagataggcg ctcacatcaa acaccgagta caggagctgg gccatggctg
     6541 ctctgctctg gtcaccaagg caggtgcctt gcagtgtagc cccagtgatg tctacaccaa
     6601 gaaggagctc atagagtgtg cccgcagagt gtcagagaag gtctcccatg ttctggctgc
     6661 actccaggct gggaatcgtg gtacccaggc ctgcattaca gcagccagtg ctgtgtctgg
     6721 tatcattgct gacctcgaca ccaccatcat gttcgctact gctggcacac ttaaccgtga
     6781 gggtgctgaa acttttgctg accaccggga gggtatctta aagacagcaa aggttcttgt
     6841 ggaggacacc aaggtcctag tgcagaatgc agctgggagc caggagaagt tggcacaagc
     6901 cgcccagtcc tccgtggcca ccattacccg cctcgctgat gtggtcaagc tcggtgcagc
     6961 cagcctagga gccgaagacc ctgaaactca ggtggtgctg atcaatgcag taaaggacgt
     7021 agccaaggcc ctgggtgacc tcatcagcgc tacgaaggct gcagcgggca aagttgggga
     7081 tgaccctgca gtgtggcagc tcaagaactc tgccaaggtg atggtgacca atgtgacatc
     7141 attgctcaag acagtgaagg ctgtggaaga tgaggccacc aaaggcacac gggccctaga
     7201 ggcaaccaca gagcacatac gtcaggaact ggcggtcttc tgttccccag agccacctgc
     7261 caagacctct acccctgaag acttcatccg aatgaccaag ggtattacta tggcaacagc
     7321 caaagccgtt gctgctggca attcctgtcg acaggaagat gtcattgcca cagccaatct
     7381 gagccgacgg gctattgcgg acatgcttcg ggcttgcaag gaagcagctt tccacccaga
     7441 agtggcgcct gatgtacggc tccgagccct gcactatggc cgggagtgtg ccaatggtta
     7501 cctggaactg ctggaccacg tgctgctgac ccttcagaag ccaaacccag acctgaagca
     7561 gcagctaact ggacactcaa agcgggttgc tggctctgtg actgagctca tccaggctgc
     7621 tgaagccatg aaaggaacag agtgggtgga cccagaggac cctactgtca ttgctgagaa
     7681 tgagctcttg ggagccgcag ccgccatcga ggctgcagcc aagaagctgg agcagctgaa
     7741 gcccagggcc aaacccaagg aggcggatga gtccttgaac tttgaggaac aaatcctaga
     7801 agctgccaag tccatcgctg cagccaccag tgcactggta aaggctgcgt cagcagccca
     7861 gagggagctg gtggctcaag gaaaggtggg cgccattcca gccaatgcac tggatgatgg
     7921 gcagtggtcg cagggcctca tttccgctgc ccgtatggtg gctgcggcca ccaacaatct
     7981 gtgtgaagca gctaacgcag ctgtccaggg ccatgctagc caggagaaac tcatttcctc
     8041 agccaagcaa gtagctgcct ccacagctca gctcctggta gcttgcaagg tcaaagcaga
     8101 tcaggactct gaggcaatga aacggctcca ggctgctggc aatgcagtga agagggcctc
     8161 agataacctg gtaaaggcgg cccagaaagc tgcagccttc gaagaccagg agaatgagac
     8221 ggtggtggtg aaggagaaaa tggttggggg cattgcccag attatcgcag cacaggaaga
     8281 gatgcttcgg aaggaacgag agctggaaga ggctcggaaa aagctcgccc agatccggca
     8341 gcagcagtac aagttcttgc cttcagagct tcgagacgag cactaaaaaa gccctgtgta
     8401 tttaatgcag acccagccca gagactgtgc ctgccactac caaagccttc tgggctgctc
     8461 aggacccaag ctgcccaacc ccagcactcc cccaaagtgc ctgccaaacc ctgggcctgg
//
