LOCUS       sequence-88445-e        2197 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Intermediate Filaments, Excitation: 503, Emission: 
            518.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2197)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Dronpa-Vimentin-N-18
FEATURES             Location/Qualifiers
     source          1..2197
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1471..1480
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1477..2154
                     /codon_start=1
                     /product="monomeric photoswitchable Dronpa-Green1 
                     fluorescent protein"
                     /label=Dronpa-Green1
                     /translation="MVSVIKPDMKIKLRMEGAVNGHPFAIEGVGLGKPFEGKQSMDLKV
                     KEGGPLPFAYDILTTVFCYGNRVFAKYPENIVDYFKQSFPEGYSWERSMNYEDGGICNA
                     TNDITLDGDCYIYEIRFDGVNFPANGPVMQKRTVKWEPSTEKLYVRDGVLKGDVNMALS
                     LEGGGHYRCDFKTTYKAKKVVQLPDYHFVDHHIEIKSHDKDYSNVNLHEHAEAHSELPR
                     QAK"
ORIGIN
        1 gacgtcagat ccgctagcgc caccatgtcc accaggtccg tgtcctcgtc ctcctaccgc
       61 aggatgttcg gcggcccggg caccgcgagc cggccgagct ccagccggag ctacgtgact
      121 acgtccaccc gcacctacag cctgggcagc gcgctgcgcc ccagcaccag ccgcagcctc
      181 tacgcctcgt ccccgggcgg cgtgtatgcc acgcgctcct ctgccgtgcg cctgcggagc
      241 agcgtgcccg gggtgcggct cctgcaggac tcggtggact tctcgctggc cgacgccatc
      301 aacaccgagt tcaagaacac ccgcaccaac gagaaggtgg agctgcagga gctgaatgac
      361 cgcttcgcca actacatcga caaggtgcgc ttcctggagc agcagaataa gatcctgctg
      421 gccgagctcg agcagctcaa gggccaaggc aagtcgcgcc tgggggacct ctacgaggag
      481 gagatgcggg agctgcgccg gcaggtggac cagctaacca acgacaaagc ccgcgtcgag
      541 gtggagcgcg acaacctggc cgaggacatc atgcgcctcc gggagaaatt gcaggaggag
      601 atgcttcaga gagaggaagc cgaaaacacc ctgcaatctt tcagacagga tgttgacaat
      661 gcgtctctgg cacgtcttga ccttgaacgc aaagtggaat ctttgcaaga agagattgcc
      721 tttttgaaga aactccacga agaggaaatc caggagctgc aggctcagat tcaggaacag
      781 catgtccaaa tcgatgtgga tgtttccaag cctgacctca cggctgccct gcgtgacgta
      841 cgtcagcaat atgaaagtgt ggctgccaag aacctgcagg aggcagaaga atggtacaaa
      901 tccaagtttg ctgacctctc tgaggctgcc aaccggaaca atgacgccct gcgccaggca
      961 aagcaggagt ccactgagta ccggagacag gtgcagtccc tcacctgtga agtggatgcc
     1021 cttaaaggaa ccaatgagtc cctggaacgc cagatgcgtg aaatggaaga gaactttgcc
     1081 gttgaagctg ctaactacca agacactatt ggccgcctgc aggatgagat tcagaatatg
     1141 aaggaggaaa tggctcgtca ccttcgtgaa taccaagacc tgctcaatgt taagatggcc
     1201 cttgacattg agattgccac ctacaggaag ctgctggaag gcgaggagag caggatttct
     1261 ctgcctcttc caaacttttc ctccctgaac ctgagggaaa ctaatctgga ttcactccct
     1321 ctggttgata cccactcaaa aaggacactt ctgattaaga cggttgaaac tagagatgga
     1381 caggttatca acgaaacttc tcagcatcac gatgaccttg aaagcagcgg tggagcaagc
     1441 gcagccagtg gtagcgcgga tccaccggtc gccaccatgg tgagtgtgat taaaccagac
     1501 atgaagatca agctgcgtat ggaaggcgct gtaaatggac acccgttcgc gattgaagga
     1561 gttggccttg ggaagccttt cgagggaaaa cagagtatgg accttaaagt caaagaaggc
     1621 ggacctctgc ctttcgccta tgacatcttg acaactgtgt tctgttacgg caacagggta
     1681 ttcgccaaat acccagaaaa tatagtagac tatttcaagc agtcgtttcc tgagggctac
     1741 tcttgggaac gaagcatgaa ttacgaagac gggggcattt gtaacgcgac aaacgacata
     1801 accctggatg gtgactgtta tatctatgaa attcgatttg atggtgtgaa ctttcctgcc
     1861 aatggtccag ttatgcagaa gaggactgtg aaatgggagc catccactga gaaattgtat
     1921 gtgcgtgatg gagtgctgaa gggtgatgtt aacatggctc tgtcgcttga aggaggtggc
     1981 cattaccgat gtgacttcaa aactacttat aaagctaaga aggttgtcca gttgccagac
     2041 tatcactttg tggaccacca cattgagatt aaaagccacg acaaagatta cagtaatgtt
     2101 aatctgcatg agcacgccga agcgcattct gagctgccga ggcaggccaa gtaatgatag
     2161 gcggccgcga ctctagatca taatcagcca taccaca
//
