LOCUS       sequence-88509-e         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane, Excitation: 505 / 569, Emission: 516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Jaqaman K, Galbraith JA, Davidson MW, Galbraith CG
  TITLE     Changes in single-molecule integrin dynamics linked to local 
            cellular behavior.
  JOURNAL   Mol Biol Cell. 2016 May 15;27(10):1561-9. doi: 
            10.1091/mbc.E16-01-0018. Epub 2016 Mar 23.
  PUBMED    27009207
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E16-01-0018"; journalName: "Mol Biol Cell"; date: 
            "2016-05-15- 15"; volume: "27"; issue: "10"; pages: "1561-9"
COMMENT     Sequence Label: mEos2-Farnesyl-5
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        37..88
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     39..59
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             133..810
                     /codon_start=1
                     /product="monomeric variant of green-to-red photoswitchable
                     fluorescent protein EosFP (McKinney et al., 2009)"
                     /label=mEos2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
ORIGIN
        1 gactttccaa aatgtcgtaa caactccgcc cccattgacg caaatgggcg gtaggcgtgt
       61 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      121 ccggtcgcca ccatgagtgc gattaagcca gacatgaaga tcaaactccg tatggaaggc
      181 aacgtaaacg ggcaccactt tgtgatcgac ggagatggta caggcaagcc ttttgaggga
      241 aaacagagta tggatcttga agtcaaagag ggcggacctc tgccttttgc ctttgatatc
      301 ctgaccactg cattccatta cggcaacagg gtattcgcca aatatccaga caacatacaa
      361 gactatttta agcagtcgtt tcctaagggg tattcgtggg aacgaagctt gactttcgaa
      421 gacgggggca tttgcattgc cagaaacgac ataacaatgg aaggggacac tttctataat
      481 aaagttcgat tttatggtac caactttccc gccaatggtc cagttatgca gaagaagacg
      541 ctgaaatggg agccctccac tgagaaaatg tatgtgcgtg atggagtgct gacgggtgat
      601 attcatatgg ctttgttgct tgaaggaaat gcccattacc gatgtgactt cagaactact
      661 tacaaagcta aggagaaggg tgtcaagtta ccaggctacc actttgtgga ccactgcatt
      721 gagattttaa gccatgacaa agattacaac aaggttaagc tgtatgagca tgctgttgct
      781 cattctggat tgcctgacaa tgccagacga tccggactca gatctaagct gaaccctcct
      841 gatgagagtg gccccggctg catgagctgc aagtgtgtgc tctcctgaga attctgcagt
      901 cgacggtacc gcgggcccgg gatccaccgg atctagataa ctgatcataa tcagccatac
      961 cacatttgta gaggttttac ttgctttaaa aaaa
//
