LOCUS       sequence-88546-e        3934 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesion Kinase, Excitation: 505 / 569, 
            Emission: 516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3934)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 3934)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEos2-PTK2-N-10
FEATURES             Location/Qualifiers
     source          1..3934
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      14..23
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3215..3895
                     /codon_start=1
                     /product="monomeric variant of green-to-red photoswitchable
                     fluorescent protein EosFP (McKinney et al., 2009)"
                     /label=mEos2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
ORIGIN
        1 cagatccgct agcgccacca tggcagctgc ttaccttgac ccccacttga atcacacacc
       61 aaattcgagt actaagactc acctgggtac tggtatggaa cgttctcctg gtgcaatgga
      121 gcgagtatta aaggtctttc attattttga aagcaatagt gagccaacca cctgggccag
      181 tattatcagg catggagatg ctactgatgt caggggcatc attcagaaga tagtggacag
      241 tcacaaagta aagcatgtgg cctgctatgg attccgcctc agtcacctgc ggtcagagga
      301 ggttcactgg cttcacgtgg atatgggcgt ctccagtgtg agggagaagt atgagcttgc
      361 tcacccacca gaggagtgga aatatgaatt gagaattcgt tatttgccaa aaggatttct
      421 aaaccagttt actgaagata agccaacttt gaatttcttc tatcaacagg tgaagagcga
      481 ttatatgtta gagatagctg atcaagtgga ccaggaaatt gctttgaagt tgggttgtct
      541 agaaatacgg cgatcatact gggagatgcg gggcaatgca ctagaaaaga agtctaacta
      601 tgaagtatta gaaaaagatg ttggtttaaa gcgatttttt cctaagagtt tactggattc
      661 tgtcaaggcc aaaacactaa gaaaactgat ccaacaaaca tttagacaat ttgccaacct
      721 taatagagaa gaaagtattc tgaaattctt tgagatcctg tctccagtct acagatttga
      781 taaggaatgc ttcaagtgtg ctcttggttc aagctggatt atttcagtgg aactggcaat
      841 cggcccagaa gaaggaatca gttacctaac ggacaagggc tgcaatccca cacatcttgc
      901 tgacttcact caagtgcaaa ccattcagta ttcaaacagt gaagacaagg acagaaaagg
      961 aatgctacaa ctaaaaatag caggtgcacc cgagcctctg acagtgacgg caccatccct
     1021 aaccattgcg gagaatatgg ctgacctaat agatgggtac tgccggctgg tgaatggaac
     1081 ctcgcagtca tttatcatca gacctcagaa agaaggtgaa cgggctttgc catcaatacc
     1141 aaagttggcc aacagcgaaa agcaaggcat gcggacacac gccgtctctg tgtcagaaac
     1201 agatgattat gctgagatta tagatgaaga agatacttac accatgccct caaccaggga
     1261 ttatgagatt caaagagaaa gaatagaact tggacgatgt attggagaag gccaatttgg
     1321 agatgtacat caaggcattt atatgagtcc agagaatcca gctttggcgg ttgcaattaa
     1381 aacatgtaaa aactgtactt cggacagcgt gagagagaaa tttcttcaag aagccttaac
     1441 aatgcgtcag tttgaccatc ctcatattgt gaagctgatt ggagtcatca cagagaatcc
     1501 tgtctggata atcatggagc tgtgcacact tggagagctg aggtcatttt tgcaagtaag
     1561 gaaatacagt ttggatctag catctttgat cctgtatgcc tatcagctta gtacagctct
     1621 tgcatatcta gagagcaaaa gatttgtaca cagggacatt gctgctcgga atgttctggt
     1681 gtcctcaaat gattgtgtaa aattaggaga ctttggatta tcccgatata tggaagatag
     1741 tacttactac aaagcttcca aaggaaaatt gcctattaaa tggatggctc cagagtcaat
     1801 caattttcga cgttttacct cagctagtga cgtatggatg tttggtgtgt gtatgtggga
     1861 gatactgatg catggtgtga agccttttca aggagtgaag aacaatgatg taatcggtcg
     1921 aattgaaaat ggggaaagat taccaatgcc tccaaattgt cctcctaccc tctacagcct
     1981 tatgacgaaa tgctgggcct atgaccccag caggcggccc aggtttactg aacttaaagc
     2041 tcagctcagc acaatcctgg aggaagagaa ggctcagcaa gaagagcgca tgaggatgga
     2101 gtccagaaga caggccacag tgtcctggga ctccggaggg tctgatgaag caccgcccaa
     2161 gcccagcaga ccgggttatc ccagtccgag gtccagcgaa ggattttatc ccagcccaca
     2221 gcacatggta caaaccaatc attaccaggt ttctggctac cctggttcac atggaatcac
     2281 agccatggct ggcagcatct atccaggtca ggcatctctt ttggaccaaa cagattcatg
     2341 gaatcataga cctcaggaga tagcaatgtg gcagcccaat gtggaggact ctacagtatt
     2401 ggacctgcga gggattgggc aagtgttgcc aacccatctg atggaagagc gtctaatccg
     2461 acagcaacag gaaatggaag aagatcagcg ctggctggaa aaagaggaaa gatttctgaa
     2521 acctgatgtg agactctctc gaggcagtat tgacagggag gatggaagtc ttcagggtcc
     2581 gattggaaac caacatatat atcagcctgt gggtaaacca gatcctgcag ctccaccaaa
     2641 gaaaccgcct cgccctggag ctcccggtca tctgggaagc cttgccagcc tcagcagccc
     2701 tgctgacagc tacaacgagg gtgtcaagcc atggaggctt cagccccagg aaatcagccc
     2761 ccctcctact gccaacctgg accggtcgaa tgataaggtg tacgagaatg tgacgggcct
     2821 ggtgaaagct gtcatcgaga tgtccagtaa aatccagcca gccccaccag aggagtatgt
     2881 ccctatggtg aaggaagtcg gcttggccct gaggacatta ttggccactg tggatgagac
     2941 cattcccctc ctaccagcca gcacccaccg agagattgag atggcacaga agctattgaa
     3001 ctctgacctg ggtgagctca tcaacaagat gaaactggcc cagcagtatg tcatgaccag
     3061 cctccagcaa gagtacaaaa agcaaatgct gactgctgct cacgccctgg ctgtggatgc
     3121 caaaaactta ctcgatgtca ttgaccaagc aagactgaaa atgcttgggc agacgagacc
     3181 acacagctcc ggaggggatc caccggtcgc caccatgagt gcgattaagc cagacatgaa
     3241 gatcaaactc cgtatggaag gcaacgtaaa cgggcaccac tttgtgatcg acggagatgg
     3301 tacaggcaag ccttttgagg gaaaacagag tatggatctt gaagtcaaag agggcggacc
     3361 tctgcctttt gcctttgata tcctgaccac tgcattccat tacggcaaca gggtattcgc
     3421 caaatatcca gacaacatac aagactattt taagcagtcg tttcctaagg ggtattcgtg
     3481 ggaacgaagc ttgactttcg aagacggggg catttgcatt gccagaaacg acataacaat
     3541 ggaaggggac actttctata ataaagttcg attttatggt accaactttc ccgccaatgg
     3601 tccagttatg cagaagaaga cgctgaaatg ggagccctcc actgagaaaa tgtatgtgcg
     3661 tgatggagtg ctgacgggtg atattcatat ggctttgttg cttgaaggaa atgcccatta
     3721 ccgatgtgac ttcagaacta cttacaaagc taaggagaag ggtgtcaagt taccaggcta
     3781 ccactttgtg gaccactgca ttgagatttt aagccatgac aaagattaca acaaggttaa
     3841 gctgtatgag catgctgttg ctcattctgg attgcctgac aatgccagac gataagcggc
     3901 cgcgactcta gatcataatc agccatacca catt
//
