LOCUS       sequence-88631-e        1191 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 505 / 569, Emission: 516
            / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1191)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 1191)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEos4a-H2B-C-10
FEATURES             Location/Qualifiers
     source          1..1191
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      39..48
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             45..725
                     /codon_start=1
                     /product="fixation-resistant green-to-red photoswitchable 
                     fluorescent protein (Paez-Segala et al., 2015)"
                     /label=mEos4a
                     /note="derived from mEos2"
                     /translation="MVSAIKPDMRIKLRMEGNVNGHHFVIDGDGTGKPYEGKQTMDLEV
                     KEGGPLPFAFDILTTAFHYGNRVFVKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICYA
                     RNDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALL
                     LEGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHAIEILSHDKDYNKVKLYEHAVAHSGLP
                     DNARR"
     CDS             756..1133
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="PEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKVL
                     KQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLPG
                     ELAKHAVSEGTKAITKYTSAK"
ORIGIN
        1 acatgctagt gacgtcagat ccgctagcgc taccggtcgc caccatggtg agtgcgatta
       61 agccagacat gaggatcaaa ctccgtatgg aaggcaacgt aaacgggcac cactttgtga
      121 tcgacggaga tggtacaggc aagccttatg agggaaaaca gaccatggat cttgaagtca
      181 aagagggcgg acctctgcct tttgcctttg atatcctgac cactgcattc cattacggca
      241 acagggtatt cgttaaatat ccagacaaca tacaagacta ttttaagcag tcgtttccta
      301 aggggtattc gtgggaacga agcttgactt tcgaagacgg gggcatttgc tatgccagaa
      361 acgacataac aatggaaggg gacactttct ataataaagt tcgattttat ggtaccaact
      421 ttcccgccaa tggtccagtt atgcagaaga agacgctgaa atgggagccc tccactgaga
      481 aaatgtatgt gcgtgatgga gtgctgacgg gtgatattca tatggctttg ttgcttgaag
      541 gaaatgccca ttaccgatgt gacttcagaa ctacttacaa agctaaggag aagggtgtca
      601 agttaccagg ctaccacttt gtggaccacg ccattgagat tttaagccat gacaaagatt
      661 acaacaaggt taagctgtat gagcatgctg ttgctcattc tggattgcct gacaatgcca
      721 gacgatccgg actcagatct ggaggctccg gaggcccaga gccagcgaag tctgctcccg
      781 ccccgaaaaa gggctccaag aaggcggtga ctaaggcgca gaagaaaggc ggcaagaagc
      841 gcaagcgcag ccgcaaggag agctattcca tctatgtgta caaggttctg aagcaggtcc
      901 accctgacac cggcatttcg tccaaggcca tgggcatcat gaattcgttt gtgaacgaca
      961 ttttcgagcg catcgcaggt gaggcttccc gcctggcgca ttacaacaag cgctcgacca
     1021 tcacctccag ggagatccag acggccgtgc gcctgctgct gcctggggag ttggccaagc
     1081 acgccgtgtc cgagggtact aaggccatca ccaagtacac cagcgctaag taaggatcca
     1141 ccggatctag ataactgatc ataatcagcc ataccacatt gtagagaagc c
//
