LOCUS       sequence-88648-e        2397 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane/Vesicles, Excitation: 505 / 569, Emission: 
            516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2397)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 2397)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEos4b-C-Src-7
FEATURES             Location/Qualifiers
     source          1..2397
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             55..96
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
     regulatory      1669..1678
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1675..2358
                     /codon_start=1
                     /product="fixation-resistant truly monomeric green-to-red 
                     photoswitchable fluorescent protein (Paez-Segala et al., 
                     2015)"
                     /label=mEos4b
                     /note="derived from mEos2"
                     /translation="MVSAIKPDMRIKLRMEGNVNGHHFVIDGDGTGKPYEGKQTMDLEV
                     KEGGPLPFAFDILTTAFHYGNRVFVKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNA
                     RNDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIEMALL
                     LEGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHAIEILSHDKDYNKVKLYEHAVAHSGLP
                     DNARR"
ORIGIN
        1 cgtcagatgc gctagcgcta ccggactcag atctcgagct caagcttcga attcatgggg
       61 agcagcaaga gcaagcccaa ggaccccagc cagcgccggc gcagcctgga gccacccgac
      121 agcacccacc acgggggatt cccagcctcg cagaccccca acaagacagc agcccccgac
      181 acgcaccgca cccccagccg ctcctttggg accgtggcca ccgagcccaa gctcttcggg
      241 ggcttcaaca cttctgacac cgtcacgtcg ccgcagcgtg ccggggcact ggctggcggc
      301 gtcaccactt tcgtggctct ctatgactac gagtcccgga ctgaaacgga cttgtccttc
      361 aagaaaggag aacgcctgca gattgtcaac aacacggaag gtgactggtg gctggctcat
      421 tccctcacta caggacagac gggctacatc cccagtaact atgtcgcgcc ctcagactcc
      481 atccaggctg aagagtggta ctttgggaag atcactcgtc gggagtccga gcggctgctg
      541 ctcaaccccg aaaacccccg gggaaccttc ttggtccggg agagcgagac gacaaaaggt
      601 gcctattgcc tctccgtttc tgactttgac aacgccaagg ggctcaatgt gaagcactac
      661 aagatccgca agctggacag cggcggcttc tacatcacct cacgcacaca gttcagcagc
      721 ctgcagcagc tggtggccta ctactccaaa catgctgatg gcttgtgcca ccgcctgacc
      781 aacgtctgcc ccacgtccaa gccccagacc cagggactcg ccaaggacgc gtgggaaatc
      841 ccccgggagt cgctgcggct ggaggtgaag ctggggcagg gctgctttgg agaggtctgg
      901 atggggacct ggaacggcac caccagagtg gccataaaga ctctgaagcc cggcaccatg
      961 tccccggagg ccttcctgca ggaagcccaa gtgatgaaga agctccggca tgagaagctg
     1021 gttcagctgt acgcagtggt gtcggaagag cccatctaca tcgtcactga gtacatgagc
     1081 aaggggagcc tcctggattt cctgaaggga gagatgggca agtacctgcg gctgccacag
     1141 ctcgtcgata tggctgctca gattgcatcc ggcatggcct atgtggagag gatgaactac
     1201 gtgcaccgag acctgcgggc ggccaacatc ctggtggggg agaacctggt gtgcaaggtg
     1261 gctgactttg ggctggcacg cctcatcgag gacaacgagt acacagcacg gcaaggtgcc
     1321 aagttcccca tcaagtggac agcccccgag gcagccctct atggccggtt caccatcaag
     1381 tcggatgtct ggtccttcgg catcctgctg actgagctga ccaccaaggg ccgggtgcca
     1441 tacccaggga tggtcaacag ggaggtgctg gaccaggtgg agaggggcta ccgcatgccc
     1501 tgcccgcccg agtgccccga gtcgctgcat gacctcatgt gccagtgctg gcggaaggac
     1561 cctgaggagc ggcccacttt tgagtacctg caggccttcc tggaggacta cttcacctcg
     1621 acagagcccc agtaccagcc tggagagaac ctacgggatc caccggtcgc caccatggtg
     1681 agtgcgatta agccagacat gaggatcaaa ctccgtatgg aaggcaacgt aaacgggcac
     1741 cactttgtga tcgacggaga tggtacaggc aagccttatg agggaaaaca gaccatggat
     1801 cttgaagtca aagagggcgg acctctgcct tttgcctttg atatcctgac cactgcattc
     1861 cattacggca acagggtatt cgtgaaatat ccagacaaca tacaagacta ttttaagcag
     1921 tcgtttccta aggggtattc gtgggaacga agcttgactt tcgaagacgg gggcatttgc
     1981 aatgccagaa acgacataac aatggaaggg gacactttct ataataaagt tcgattttat
     2041 ggtaccaact ttcccgccaa tggtccagtt atgcagaaga agacgctgaa atgggagccc
     2101 tccactgaga aaatgtatgt gcgtgatgga gtgctgacgg gtgatattga gatggctttg
     2161 ttgcttgaag gaaatgccca ttaccgatgt gacttcagaa ctacttacaa agctaaggag
     2221 aagggtgtca agttaccagg cgcccacttt gtggaccacg ccattgagat tttaagccat
     2281 gacaaagatt acaacaaggt taagctgtat gagcatgctg ttgctcattc tggattgcct
     2341 gacaatgcca gacgataagc ggccgcgact ctagatcata atcagccata ccacatg
//
