LOCUS       sequence-88703-e        1398 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Peroxisomes, Excitation: 503 / 569, Emission: 514 / 
            581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1398)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 1398)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mGeos2-VEL-PMP-N-10
FEATURES             Location/Qualifiers
     source          1..1398
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      47..56
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             668..1348
                     /codon_start=1
                     /product="truly monomeric reversibly photoswitchable 
                     fluorescent protein, suitable for nonlinear structured 
                     illumination microscopy (Zhang et al., 2016)"
                     /label=Skylan-NS
                     /note="mEos3.1 derivative that eliminates the green-to-red 
                     conversion"
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFLYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDVEMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
ORIGIN
        1 acatgctagt gacgtcagat ccgctagcgc taccggactc agatctgcca ccatggcgcc
       61 ggccgcgtcc aggctgcggg ccgaagccgg gctcggggcg ctgccgcggc gggcgctcgc
      121 ccagtacttg ctcttcctgc ggctctaccc ggtgctcacc aaggcggcca ccagtggcat
      181 tttgtcagca cttgggaact tcctggccca gatgattgag aagaagcgga aaaaagaaaa
      241 ctctagaagt ctggatgtcg gtgggcctct gagatatgcc gtttacgggt tcttcttcac
      301 agggccgctg agtcacttct tctacttctt catggaacat tggatccctc ctgaggtccc
      361 cctggcaggg ctcaggaggc ttctcctgga ccgcctcgtc tttgcaccgg ccttcctcat
      421 gttgttcttc ctcatcatga actttctgga ggggaaagac gcctcagcct tcgccgccaa
      481 gatgaggggg ggcttctggc cggcgctgag gatgaactgg cgggtgtgga cgccactaca
      541 gttcatcaac atcaactacg tccctctgaa gttccgggtg ctcttcgcca acctggcagc
      601 tctgttctgg tatgcctacc tggcctcctt ggggaagagc ggctccggta gcggaccggt
      661 cgccaccatg agtgcgatta agccagacat gaagatcaaa ctccgtatgg aaggcaacgt
      721 aaacgggcac cactttgtga tcgacggaga tggtacaggc aagccttttg agggaaaaca
      781 gagtatggat cttgaagtca aagagggcgg acctctgcct tttgcctttg atatcctgac
      841 cactgcattc ctgtacggca acagggtatt cgccaaatat ccagacaaca tacaagacta
      901 ttttaagcag tcgtttccta aggggtattc gtgggaacga agcttgactt tcgaagacgg
      961 gggcatttgc aacgccagaa acgacataac aatggaaggg gacactttct ataataaagt
     1021 tcgattttat ggtaccaact ttcccgccaa tggtccagtt atgcagaaga agacgctgaa
     1081 atgggagccc tccactgaga aaatgtatgt gcgtgatgga gtgctgacgg gtgatgtaga
     1141 gatggctttg ttgcttgaag gaaatgccca ttaccgatgt gacttcagaa ctacttacaa
     1201 agctaaggag aagggtgtca agttaccagg cgcccacttt gtggaccact gcattgagat
     1261 tttaagccat gacaaagatt acaacaaggt taagctgtat gagcatgctg ttgctcattc
     1321 tggattgcct gacaatgcca gacgataagc ggccgcgact ctagatcata atcagccata
     1381 ccacatgtag agtagccc
//
