LOCUS       sequence-8872-ex        6325 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6325)
  AUTHORS   Brandman O, Liou J, Park WS, Meyer T
  TITLE     STIM2 is a feedback regulator that stabilizes basal cytosolic and 
            endoplasmic reticulum Ca2+ levels.
  JOURNAL   Cell. 2007 Dec 28. 131(7):1327-39.
  PUBMED    18160041
REFERENCE   2  (bases 1 to 6325)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2007 
            Dec 28. 131(7):1327-39."
COMMENT     Sequence Label: pEX-CMV-SP-STIM2(15-746)
FEATURES             Location/Qualifiers
     source          1..6325
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    complement(2896..2920)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    2924..2989
                     /label=MCS
                     /note="multiple cloning site"
     polyA_signal    3113..3234
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3150..3169)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3204..3223
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3241..3696)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3378..3399)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3590..3609
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3723..3827
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3829..4186
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3829..4020
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3852..3872)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4037..4172
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4221..5015
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4221..5015
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4275..4294)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4885..4904
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5203..5222)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5247..5294
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5623..6211
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6112..6131
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcacca
      601 tggatgtatg cgtccgtctt gccctgtggc tcctctgggg actcctcctg caccagggcc
      661 agagcctcgc accggtcaaa aaagcaggct ccctctgcga gcttgtgccc cggcacctcc
      721 gcgggcggcg ggcgactggc tctgccgcaa ctgccgcctc ctctctcgcc gcggcggccg
      781 gcgatagccc ggcgctcatg acagatccct gcatgtcact gagtccacca tgctttacag
      841 aagaagacag atttagtctg gaagctcttc aaacaataca taaacaaatg gatgatgaca
      901 aagatggtgg aattgaagta gaggaaagtg atgaattcat cagagaagat atgaaatata
      961 aagatgctac taataaacac agccatctgc acagagaaga taaacatata acgattgagg
     1021 atttatggaa acgatggaaa acatcagaag ttcataattg gacccttgaa gacactcttc
     1081 agtggttgat agagtttgtt gaactacccc aatatgagaa gaattttaga gacaacaatg
     1141 tcaaaggaac gacacttccc aggatagcag tgcacgaacc ttcatttatg atctcccagt
     1201 tgaaaatcag tgaccggagt cacagacaaa aacttcagct caaggcattg gatgtggttt
     1261 tgtttggacc tctaacacgc ccacctcata actggatgaa agattttatc ctcacaattt
     1321 ctatagtaat tggtgttgga ggctgctggt ttgcttatac gcagaataag acatcaaaag
     1381 aacatgttgc aaaaatgatg aaagatttag agagcttaca aactgcagag caaagtctaa
     1441 tggacttaca agagaggctt gaaaaggcac aggaagaaaa cagaaatgtt gctgtagaaa
     1501 agcaaaattt agagcgcaaa atgatggatg aaatcaatta tgcaaaggag gaggcttgtc
     1561 ggctgagaga gctaagggag ggagctgaat gtgaattgag tagacgtcag tatgcagaac
     1621 aggaattgga acaggttcgc atggctctga aaaaggccga aaaagaattt gaactgagaa
     1681 gcagttggtc tgttccagat gcacttcaga aatggcttca gttaacacat gaagtagaag
     1741 tgcaatacta caatattaaa agacaaaacg ctgaaatgca gctagctatt gctaaagatg
     1801 aggcagaaaa aattaaaaag aagagaagca cagtctttgg gactctgcac gttgcacaca
     1861 gctcctccct agatgaggta gaccacaaaa ttctggaagc aaagaaagct ctctctgagt
     1921 tgacaacttg tttacgagaa cgactttttc gctggcaaca aattgagaag atctgtggct
     1981 ttcagatagc ccataactca ggactcccca gcctgacctc ttccctttat tctgatcaca
     2041 gctgggtggt gatgcccaga gtctccattc caccctatcc aattgctgga ggagttgatg
     2101 acttagatga agacacaccc ccaatagtgt cacaatttcc cgggaccatg gctaaacctc
     2161 ctggatcatt agccagaagc agcagcctgt gccgttcacg ccgcagcatt gtgccgtcct
     2221 cgcctcagcc tcagcgagct cagcttgctc cacacgcccc ccacccgtca caccctcggc
     2281 accctcacca cccgcaacac acaccacact ccttgccttc ccctgatcca gatatcctct
     2341 cagtgtcaag ttgccctgcg ctttatcgaa atgaagtgga ggaagaggcc atttacttct
     2401 ctgctgaaaa gcaatgggaa gtgccagaca cagcttcaga atgtgactcc ttaaattctt
     2461 ccattggaag gaaacagtct cctcctttaa gcctcgagat ataccaaaca ttatctccgc
     2521 gaaagatatc aagagatgag gtgtccctag aggattcctc ccgaggggat tcgcctgtaa
     2581 ctgtggatgt gtcttggggt tctcccgact gtgtaggtct gacagagact aagagtatga
     2641 tcttcagtcc tgcaagcaaa gtgtacaatg gcattttgga gaaatcctgt agcatgaacc
     2701 agctttccag tggcatcccg gtgcctaaac ctcgccacac atcatgttcc tcagctggca
     2761 acgacagtaa accagttcag gaagccccaa gtgttgccag aataagcagc atcccacatg
     2821 acctttgtca taatggagag aaaagcaaaa agccatcaaa aatcaaaagc ctttttaaga
     2881 agaaatctaa gtgaaaccca gctttcttgt acaaagtggt tgatccggac tcagatctcg
     2941 agctcaagct tcgaattctg cagtcgacgg taccgcgggc ccgggatcca ccggatctag
     3001 ataactgatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct
     3061 cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt
     3121 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     3181 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttaacgcgt
     3241 aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
     3301 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
     3361 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
     3421 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct
     3481 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
     3541 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
     3601 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
     3661 cacccgccgc gcttaatgcg ccgctacagg gcgcgtcagg tggcactttt cggggaaatg
     3721 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     3781 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtcct gaggcggaaa
     3841 gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
     3901 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     3961 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
     4021 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     4081 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     4141 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaagatc gatcaagaga
     4201 caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg
     4261 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg
     4321 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt
     4381 ccggtgccct gaatgaactg caagacgagg cagcgcggct atcgtggctg gccacgacgg
     4441 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat
     4501 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat
     4561 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg
     4621 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg
     4681 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc
     4741 tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc
     4801 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg
     4861 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg
     4921 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca
     4981 tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac
     5041 cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga
     5101 aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga
     5161 tctcatgctg gagttcttcg cccaccctag ggggaggcta actgaaacac ggaaggagac
     5221 aataccggaa ggaacccgcg ctatgacggc aataaaaaga cagaataaaa cgcacggtgt
     5281 tgggtcgttt gttcataaac gcggggttcg gtcccagggc tggcactctg tcgatacccc
     5341 accgagaccc cattggggcc aatacgcccg cgtttcttcc ttttccccac cccacccccc
     5401 aagttcgggt gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc ctgccatagc
     5461 ctcaggttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     5521 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     5581 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     5641 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     5701 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     5761 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     5821 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     5881 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     5941 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     6001 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     6061 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     6121 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     6181 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     6241 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     6301 ggataaccgt attaccgcca tgcat
//
