LOCUS       sequence-88762-e        2343 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: MT End Binding Protein, Excitation: 505 / 569, 
            Emission: 516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2343)
  AUTHORS   Shtengel G, Galbraith JA, Galbraith CG, Lippincott-Schwartz J, 
            Gillette JM, Manley S, Sougrat R, Waterman CM, Kanchanawong P, 
            Davidson MW, Fetter RD, Hess HF
  TITLE     Interferometric fluorescent super-resolution microscopy resolves 3D 
            cellular ultrastructure.
  JOURNAL   Proc Natl Acad Sci U S A. 2009 Mar 3;106(9):3125-30. doi: 
            10.1073/pnas.0813131106. Epub 2009 Feb 6.
  PUBMED    19202073
REFERENCE   2  (bases 1 to 2343)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0813131106"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2009-03-3- 3"; volume: "106"; issue: "9"; pages: "3125-30"
COMMENT     Sequence Label: tdEos-EB3-7
FEATURES             Location/Qualifiers
     source          1..2343
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      53..62
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             923..1600
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="MSAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     CDS             1649..2326
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="SAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVKE
                     GGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIARN
                     DITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLLE
                     GNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPDN
                     ARR"
ORIGIN
        1 agcgctaccg gactcagatc tcgagctcaa gcttcgaatt ctgcagtcga ctgccaccat
       61 ggccgtcaat gtgtactcca catctgtgac cagtgaaaat ctgagtcgcc atgatatgct
      121 tgcatgggtc aacgactccc tgcacctcaa ctataccaag atagaacagc tttgttcagg
      181 ggcagcctac tgccagttca tggacatgct cttccccggc tgtgtgcact tgaggaaagt
      241 gaagttccag gccaaactag agcatgaata catccacaac ttcaaggtgc tgcaagcagc
      301 tttcaagaag atgggtgttg acaaaatcat tcctgtagag aaattagtga aaggaaaatt
      361 ccaagataat tttgagttta ttcagtggtt taagaaattc tttgacgcaa actatgatgg
      421 aaaggattac aaccctctgc tggcgcggca gggccaggac gtagcgccac ctcctaaccc
      481 aggtgatcag atcttcaaca aatccaagaa actcattggc acagcagttc cacagaggac
      541 gtcccccaca ggcccaaaaa acatgcagac ctctggccgg ctgagcaatg tggccccccc
      601 ctgcattctc cggaagaatc ctccatcagc ccgaaatggc ggccatgaga ctgatgccca
      661 aattcttgaa ctcaaccaac agctggtgga cttgaagctg acagtggatg ggctggagaa
      721 ggaacgtgac ttctacttca gcaaacttcg tgacatcgag ctcatctgcc aggagcatga
      781 aagtgaaaac agccctgtta tctcaggcat cattggcatc ctctatgcca cagaggaagg
      841 attcgcaccc cctgaggacg atgagattga agagcatcaa caagaagacc aggacgagta
      901 cctggatcca ccggtcgcca ccatgagtgc gattaagcca gacatgaaga tcaacctccg
      961 tatggaaggc aacgtaaacg ggcaccactt tgtgatcgac ggagatggta caggcaagcc
     1021 ttttgaggga aaacagagta tggatcttga agtcaaagag ggcggacctc tgccttttgc
     1081 ctttgatatc ctgaccactg cattccatta cggcaacagg gtattcgccg aatatccaga
     1141 ccacatacaa gactatttta agcagtcgtt tcctaagggg tattcgtggg aacgaagctt
     1201 gactttcgaa gacgggggca tttgcattgc cagaaacgac ataacaatgg aaggggacac
     1261 tttctataat aaagttcgat ttcacggtgt aaactttccc gccaatggtc cagttatgca
     1321 gaagaagacg ctgaaatggg agccctccac tgagaaaatg tatgtgcgtg atggagtgct
     1381 gactggtgat attcgcatgg ctttgttgct tgaaggaaat gcccattacc gatgtgactt
     1441 cagaactact tacaaagcta aggagaaggg tgtcaagtta ccaggctacc actttgtgga
     1501 ccactgcatt gagattttaa gccatgacaa agattacaac aaggttaagc tgtatgagca
     1561 tgctgttgct cattctggat tgcctgacaa tgccagacga tccggtagcg gtcacggtac
     1621 tggttctact ggttctggtt cttctgagag tgcgattaag ccagacatga agatcaacct
     1681 ccgtatggaa ggcaacgtaa acgggcacca ctttgtgatc gacggagatg gtacaggcaa
     1741 gccttttgag ggaaaacaga gtatggatct tgaagtcaaa gagggcggac ctctgccttt
     1801 tgcctttgat atcctgacca ctgcattcca ttacggcaac agggtattcg ccgaatatcc
     1861 agaccacata caagactatt ttaagcagtc gtttcctaag gggtattcgt gggaacgaag
     1921 cttgactttc gaagacgggg gcatttgcat tgccagaaac gacataacaa tggaagggga
     1981 cactttctat aataaagttc gatttcacgg tgtaaacttt cccgccaatg gtccagttat
     2041 gcagaagaag acgctgaaat gggagccctc cactgagaaa atgtatgtgc gtgatggagt
     2101 gctgactggt gatattcgca tggctttgtt gcttgaagga aatgcccatt accgatgtga
     2161 cttcagaact acttacaaag ctaaggagaa gggtgtcaag ttaccaggct accactttgt
     2221 ggaccactgc attgagattt taagccatga caaagattac aacaaggtta agctgtatga
     2281 gcatgctgtt gctcattctg gattgcctga caatgccaga cgataagcgg ccgcgactct
     2341 aga
//
