LOCUS       sequence-88811-e        3314 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Centrosomes, Excitation: 505 / 569, Emission: 516 / 
            581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3314)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 3314)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdEos-PLK1-N-16
FEATURES             Location/Qualifiers
     source          1..3314
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1875..2552
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="MSAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     CDS             2601..3278
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="SAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVKE
                     GGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIARN
                     DITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLLE
                     GNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPDN
                     ARR"
ORIGIN
        1 gatccgctag cgccaccatg agtgctgcag tgactgcagg gaagctggca cgggcaccgg
       61 ccgaccctgg gaaagccggg gtccccggag ttgcagctcc cggagctccg gcggcggctc
      121 caccggcgaa agagatcccg gaggtcctag tggacccacg cagccggcgg cgctatgtgc
      181 ggggccgctt tttgggcaag ggcggctttg ccaagtgctt cgagatctcg gacgcggaca
      241 ccaaggaggt gttcgcgggc aagattgtgc ctaagtctct gctgctcaag ccgcaccaga
      301 gggagaagat gtccatggaa atatccattc accgcagcct cgcccaccag cacgtcgtag
      361 gattccacgg ctttttcgag gacaacgact tcgtgttcgt ggtgttggag ctctgccgcc
      421 ggaggtctct cctggagctg cacaagagga ggaaagccct gactgagcct gaggcccgat
      481 actacctacg gcaaattgtg cttggctgcc agtacctgca ccgaaaccga gttattcatc
      541 gagacctcaa gctgggcaac cttttcctga atgaagatct ggaggtgaaa ataggggatt
      601 ttggactggc aaccaaagtc gaatatgacg gggagaggaa gaagaccctg tgtgggactc
      661 ctaattacat agctcccgag gtgctgagca agaaagggca cagtttcgag gtggatgtgt
      721 ggtccattgg gtgtatcatg tataccttgt tagtgggcaa accacctttt gagacttctt
      781 gcctaaaaga gacctacctc cggatcaaga agaatgaata cagtattccc aagcacatca
      841 accccgtggc cgcctccctc atccagaaga tgcttcagac agatcccact gcccgcccaa
      901 ccattaacga gctgcttaat gacgagttct ttacttctgg ctatatccct gcccgtctcc
      961 ccatcacctg cctgaccatt ccaccaaggt tttcgattgc tcccagcagc ctggacccca
     1021 gcaaccggaa gcccctcaca gtcctcaata aaggcttgga gaaccccctg cctgagcgtc
     1081 cccgggaaaa agaagaacca gtggttcgag agacaggtga ggtggtcgac tgccacctca
     1141 gtgacatgct gcagcagctg cacagtgtca atgcctccaa gccctcggag cgtgggctgg
     1201 tcaggcaaga ggaggctgag gatcctgcct gcatccccat cttctgggtc agcaagtggg
     1261 tggactattc ggacaagtac ggccttgggt atcagctctg tgataacagc gtgggggtgc
     1321 tcttcaatga ctcaacacgc ctcatcctct acaatgatgg tgacagcctg cagtacatag
     1381 agcgtgacgg cactgagtcc tacctcaccg tgagttccca tcccaactcc ttgatgaaga
     1441 agatcaccct ccttaaatat ttccgcaatt acatgagcga gcacttgctg aaggcaggtg
     1501 ccaacatcac gccgcgcgaa ggtgatgagc tcgcccggct gccctaccta cggacctggt
     1561 tccgcacccg cagcgccatc atcctgcacc tcagcaacgg cagcgtgcag atcaacttct
     1621 tccaggatca caccaagctc atcttgtgcc cactgatggc agccgtgacc tacatcgacg
     1681 agaagcggga cttccgcaca taccgcctga gtctcctgga ggagtacggc tgctgcaagg
     1741 agctggccag ccggctccgc tacgcccgca ctatggtgga caagctgctg agctcacgct
     1801 cggccagcaa ccgtctcaag gcctccggta gcggcagcgc atccggcgga agcggaagcg
     1861 gaccggtcgc caccatgagt gcgattaagc cagacatgaa gatcaacctc cgtatggaag
     1921 gcaacgtaaa cgggcaccac tttgtgatcg acggagatgg tacaggcaag ccttttgagg
     1981 gaaaacagag tatggatctt gaagtcaaag agggcggacc tctgcctttt gcctttgata
     2041 tcctgaccac tgcattccat tacggcaaca gggtattcgc cgaatatcca gaccacatac
     2101 aagactattt taagcagtcg tttcctaagg ggtattcgtg ggaacgaagc ttgactttcg
     2161 aagacggggg catttgcatt gccagaaacg acataacaat ggaaggggac actttctata
     2221 ataaagttcg atttcacggt gtaaactttc ccgccaatgg tccagttatg cagaagaaga
     2281 cgctgaaatg ggagccctcc actgagaaaa tgtatgtgcg tgatggagtg ctgactggtg
     2341 atattcgcat ggctttgttg cttgaaggaa atgcccatta ccgatgtgac ttcagaacta
     2401 cttacaaagc taaggagaag ggtgtcaagt taccaggcta ccactttgtg gaccactgca
     2461 ttgagatttt aagccatgac aaagattaca acaaggttaa gctgtatgag catgctgttg
     2521 ctcattctgg attgcctgac aatgccagac gatccggtag cggtcacggt actggttcta
     2581 ctggttctgg ttcttctgag agtgcgatta agccagacat gaagatcaac ctccgtatgg
     2641 aaggcaacgt aaacgggcac cactttgtga tcgacggaga tggtacaggc aagccttttg
     2701 agggaaaaca gagtatggat cttgaagtca aagagggcgg acctctgcct tttgcctttg
     2761 atatcctgac cactgcattc cattacggca acagggtatt cgccgaatat ccagaccaca
     2821 tacaagacta ttttaagcag tcgtttccta aggggtattc gtgggaacga agcttgactt
     2881 tcgaagacgg gggcatttgc attgccagaa acgacataac aatggaaggg gacactttct
     2941 ataataaagt tcgatttcac ggtgtaaact ttcccgccaa tggtccagtt atgcagaaga
     3001 agacgctgaa atgggagccc tccactgaga aaatgtatgt gcgtgatgga gtgctgactg
     3061 gtgatattcg catggctttg ttgcttgaag gaaatgccca ttaccgatgt gacttcagaa
     3121 ctacttacaa agctaaggag aagggtgtca agttaccagg ctaccacttt gtggaccact
     3181 gcattgagat tttaagccat gacaaagatt acaacaaggt taagctgtat gagcatgctg
     3241 ttgctcattc tggattgcct gacaatgcca gacgataagc ggccgcgact ctagatcata
     3301 atcagccata ccac
//
