LOCUS       sequence-88832-e        6717 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 
            / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6717)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 6717)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdEos-Tensin1-C-14
FEATURES             Location/Qualifiers
     source          1..6717
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             26..703
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="MSAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     CDS             752..1426
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="SAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVKE
                     GGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIARN
                     DITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLLE
                     GNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPDN
                     ARR"
ORIGIN
        1 tccgctagcg ctaccggtcg ccaccatgag tgcgattaag ccagacatga agatcaacct
       61 ccgtatggaa ggcaacgtaa acgggcacca ctttgtgatc gacggagatg gtacaggcaa
      121 gccttttgag ggaaaacaga gtatggatct tgaagtcaaa gagggcggac ctctgccttt
      181 tgcctttgat atcctgacca ctgcattcca ttacggcaac agggtattcg ccgaatatcc
      241 agaccacata caagactatt ttaagcagtc gtttcctaag gggtattcgt gggaacgaag
      301 cttgactttc gaagacgggg gcatttgcat tgccagaaac gacataacaa tggaagggga
      361 cactttctat aataaagttc gatttcacgg tgtaaacttt cccgccaatg gtccagttat
      421 gcagaagaag acgctgaaat gggagccctc cactgagaaa atgtatgtgc gtgatggagt
      481 gctgactggt gatattcgca tggctttgtt gcttgaagga aatgcccatt accgatgtga
      541 cttcagaact acttacaaag ctaaggagaa gggtgtcaag ttaccaggct accactttgt
      601 ggaccactgc attgagattt taagccatga caaagattac aacaaggtta agctgtatga
      661 gcatgctgtt gctcattctg gattgcctga caatgccaga cgatccggta gcggtcacgg
      721 tactggttct actggttctg gttcttctga gagtgcgatt aagccagaca tgaagatcaa
      781 cctccgtatg gaaggcaacg taaacgggca ccactttgtg atcgacggag atggtacagg
      841 caagcctttt gagggaaaac agagtatgga tcttgaagtc aaagagggcg gacctctgcc
      901 ttttgccttt gatatcctga ccactgcatt ccattacggc aacagggtat tcgccgaata
      961 tccagaccac atacaagact attttaagca gtcgtttcct aaggggtatt cgtgggaacg
     1021 aagcttgact ttcgaagacg ggggcatttg cattgccaga aacgacataa caatggaagg
     1081 ggacactttc tataataaag ttcgatttca cggtgtaaac tttcccgcca atggtccagt
     1141 tatgcagaag aagacgctga aatgggagcc ctccactgag aaaatgtatg tgcgtgatgg
     1201 agtgctgact ggtgatattc gcatggcttt gttgcttgaa ggaaatgccc attaccgatg
     1261 tgacttcaga actacttaca aagctaagga gaagggtgtc aagttaccag gctaccactt
     1321 tgtggaccac tgcattgaga ttttaagcca tgacaaagat tacaacaagg ttaagctgta
     1381 tgagcatgct gttgctcatt ctggattgcc tgacaatgcc agacgatccg gaagtgctgg
     1441 gagcgcaagc ggaagcggag gctccggtag tgtgagccgg accatggagg acagctgtga
     1501 gctggacctg gtgtacgtca cagagaggat catcgctgtc tccttcccca gcacagccaa
     1561 tgaggagaac tttcggagca acctccgtga ggtggcgcag atgctcaagt ccaaacatgg
     1621 aggcaactac ctgctgttca acctctctga gcggagacct gacatcacga agctccatgc
     1681 caaggtactg gaatttggct ggcccgacct ccacacccca gccctggaga agatatgcag
     1741 catctgtaag gccatggaca catggctcaa tgcagaccct cacaatgtcg ttgttctaca
     1801 caacaaggga aaccgaggca ggataggagt tgtcatcgcg gcttacatgc actacagcaa
     1861 catttctgcc agtgcggacc aggctctgga tcggtttgca atgaagcggt tctatgagga
     1921 taagattgtg cccattggcc agccatccca aagaaggtac gtgcattact tcagtggcct
     1981 gctctccggc tccatcaaaa tgaacaacaa gcccttgttt ctgcaccacg tgatcatgca
     2041 cggcatcccc aactttgagt ctaaaggagg atgtcggcca tttctccgca tctaccaggc
     2101 catgcaacct gtgtacacat ctggcatcta caacatccca ggagacagcc agactagcgt
     2161 ctgcatcacc atcgagccag gactgctctt gaagggagac atcttgctga agtgctacca
     2221 caagaagttc cgaagcccag cccgagacgt catcttccgt gtgcagttcc acacctgtgc
     2281 catccatgac ctgggggttg tctttgggaa ggaggacctt gatgatgctt tcaaagatga
     2341 tcgatttcca gagtatggca aagtggagtt tgtattttct tatgggccag agaaaattca
     2401 aggcatggag cacctggaga acgggccgag cgtgtctgtg gactataaca cctccgaccc
     2461 cctcatccgc tgggactcct acgacaactt cagtgggcat cgagatgacg gcatggagga
     2521 ggtggtggga cacacgcagg ggccactaga tgggagcctg tatgctaagg tgaagaagaa
     2581 agactccctg cacggcagca ccggggctgt taatgccaca cgtcctacac tgtcggccac
     2641 ccccaaccac gtggaacaca cgctttctgt gagcagcgac tcgggcaact ccacagcctc
     2701 caccaagacc gacaagaccg acgagcctgt ccccggggcc tccagtgcca ctgctgcctt
     2761 gagtccccag gagaagcggg agctggaccg cctgctgagt ggctttggct tagagcgaga
     2821 gaagcaaggc gccatgtacc acacccagca cctcaggtcc cgcccagcag ggggctcggc
     2881 tgtgccctcc tctggacgcc acgttgtccc agcccaggtt catgtcaatg gtggggcgtt
     2941 agcatctgag cgggagacag acatcctgga cgatgaattg ccaaaccagg atggtcacag
     3001 tgcgggcagc atgggcacac tctcttctct ggacggggtc accaacacca gtgagggggg
     3061 ctacccagag gccctgtccc cactgaccaa cggtctggac aagtcctacc ccatggagcc
     3121 tatggtcaat ggaggaggct acccctacga gtctgccagc cgggcggggc ctgcccatgc
     3181 tggccacacg gcccccatgc ggccctccta ctctgcacag gagggtttag ctggctacca
     3241 gagggagggg ccccacccag cctggccaca gccagtgacc acctcccact atgcccatga
     3301 ccccagcggt atgttccgct ctcaatcctt ttcggaagct gaaccccagc tgcccccagc
     3361 tccggtccga gggggaagca gccgggaggc tgtgcaaagg ggactgaact cgtggcagca
     3421 gcagcagcag cagcagcagc agcctcgccc acctccacgc cagcaggaaa gagcccactt
     3481 ggagagtctt gtagccagca ggcccagccc tcagccattg gcagagaccc ccatccccag
     3541 tctccctgag ttcccgcgag cagcctccca gcaggagatt gaacagtcca tcgaaacact
     3601 caatatgctg atgctggacc tggagccagc ctccgctgct gccccactac acaagtccca
     3661 gagtgtcccc ggggcctggc caggggcttc tccactctcc tcccagcccc tctctgggtc
     3721 ctcccgtcag tcccatccac tgacccagtc cagatatggc tatatcccca gtgggcattc
     3781 gttgggaacc cctgagccag ccccacgggc ctctctggag tctgtccctc ctggcaggtc
     3841 ttactcacct tatgactatc agccatgttt ggctgggcct aaccaggatt tccattcaaa
     3901 gagcccagcc tcttcctcct tgcctgcctt ccttccgacc acccacagcc ctccagggcc
     3961 tcagcaaccc ccagcctctc tccctggcct cactgctcag cctctgctct caccaaagga
     4021 agcgacttca gacccctccc ggactccaga ggaggagcca ttgaatttag aagggctggt
     4081 ggcccacagg gtagcagggg tacaggctcg ggagaagcag cctgcagagc ccccagcccc
     4141 tctgcggagg cgggcggcca gtgatggaca gtatgagaac cagtctccag aagccacatc
     4201 ccctcgtagc cctggggttc gctcccctgt ccagtgtgtc tccccggagc tggctcttac
     4261 catcgctctc aatcctggag ggcggcccaa agagccccat ttgcacagct acaaggaggc
     4321 cttcgaggag atggagggaa cctccccgag cagcccacca cccagtgggg tgcggtcccc
     4381 cccgggtctg gccaagacac ccctgtctgc tctgggcctg aaacctcaca acccagcgga
     4441 catcctgttg caccccacag gagttaccag aagacggatt cagccagagg aagatgaggg
     4501 gaaggtggtg gtcaggctgt cagaagagcc ccggagctat gtggagtctg tggcacggac
     4561 agcggtggct ggaccccgag ctcaggactc tgagcccaag agctttagtg ctccagccac
     4621 ccaggcctat ggccatgaga tacccctgag gaacgggacc ctgggtggct cctttgtctc
     4681 ccccagcccc ctctccacca gcagccccat cctcagtgct gacagcactt cagtggggag
     4741 tttcccgtcg ggagagagca gtgaccaggg tccccggacg cccacccagc ctctgttgga
     4801 gtctggcttc cgctcaggca gcctgggaca gcccagcccg tctgcccaga gaaactacca
     4861 gagctcttct cctctcccga ctgtgggcag tagctacagc agccccgact actcacttca
     4921 gcatttcagc tcctctccag aaagccaggc tcgcgctcag ttcagtgtgg ctggcgtcca
     4981 cacggtgcct gggagccctc aggcgcgcca cagaacagtg ggcaccaaca ctccccctag
     5041 tcctggcttc ggctggcggg ccatcaatcc cagcatggct gcccccagca gtcccagttt
     5101 gagccatcac cagatgatgg gtccaccagg cactggcttc catggtagca ctgtctccag
     5161 cccccagagc agtgcagcga ccaccccggg gagccccagc ctgtgtcggc acccagcagg
     5221 ggtctaccag gtttctggcc tccacaacaa agtggccacc accccgggga gtcccagcct
     5281 gggccggcac cctggggctc accaaggcaa cctggcctcc ggtcttcata gcaatgcaat
     5341 agccagccct ggaagcccca gcctgggccg tcacctcgga gggtctggat ctgtggttcc
     5401 cggcagcccc tgcttggacc ggcatgtggc ctatggtggc tattctaccc cggaggatcg
     5461 gagacccaca ctgtcccggc agagcagtgc ctctggctac caggctcctt ccacgccctc
     5521 cttccctgtc tcccctgcct actaccctgg cctgagcagc cctgccacct ccccgtcacc
     5581 agactccgca gccttccggc aagggagccc aacaccagcc ttgccagaga agcgaaggat
     5641 gtcagtggga gaccgggcag gcagcctccc caactatgcc accatcaatg ggaaggtgtc
     5701 ttcgcctgtc gccagcggca tgtccagtcc cagtgggggc agcaccgtct ccttctccca
     5761 cactctgccc gacttctcca agtactccat gccagacaac agcccggaga cgcgggctaa
     5821 agtgaagttt gtccaggaca cttctaagta ttggtacaag cctgagattt ccagggagca
     5881 ggccatcgcg ctcctcaagg accaggagcc gggggccttc atcatccgcg acagtcactc
     5941 cttccgaggc gcgtacgggc tggccatgaa ggtgtcttcg ccacctccaa ccatcatgca
     6001 gcagaataaa aaaggagaca tgacccatga gctggtcagg cattttctga tagagactgg
     6061 ccccagagga gtcaagctca agggctgccc caatgagcca aacttcggat cgctgtctgc
     6121 cctggtctac cagcactcca tcatcccatt ggccctgcct tgcaagctgg tcattccaaa
     6181 ccgagacccc acagatgaat cgaaagatag ctccggccct gccaactcaa ctgcagacct
     6241 gctgaaacaa ggggcagcct gcaatgtgct cttcgtcaac tctgtggaca tggagtcact
     6301 cactgggcca caggccatct ctaaagccac atctgagacg ttggctgcag accccacgcc
     6361 agctgccacc atcgttcact tcaaagtctc tgcccaggga atcactctga ctgacaacca
     6421 gagaaagctc tttttcagac gccactaccc tctcaacact gtcaccttct gtgacctgga
     6481 cccacaggaa agaaagtgga tgaaaacaga gggtggtgcc cctgctaagc tcttcggctt
     6541 cgtggcccgg aagcagggca gcaccacgga caacgcctgc cacctctttg ctgagcttga
     6601 ccccaaccag ccggcctctg ccatcgtcaa cttcgtctcc aaggtcatgc tgaatgccgg
     6661 ccaaaagaga tgaggatcca ccggatctag ataactgatc ataatcagcc ataccac
//
