LOCUS       sequence-88847-e        3072 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Golgi/ER Transport, Excitation: 505 / 569, Emission: 
            516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3072)
  AUTHORS   Shtengel G, Galbraith JA, Galbraith CG, Lippincott-Schwartz J, 
            Gillette JM, Manley S, Sougrat R, Waterman CM, Kanchanawong P, 
            Davidson MW, Fetter RD, Hess HF
  TITLE     Interferometric fluorescent super-resolution microscopy resolves 3D 
            cellular ultrastructure.
  JOURNAL   Proc Natl Acad Sci U S A. 2009 Mar 3;106(9):3125-30. doi: 
            10.1073/pnas.0813131106. Epub 2009 Feb 6.
  PUBMED    19202073
REFERENCE   2  (bases 1 to 3072)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0813131106"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2009-03-3- 3"; volume: "106"; issue: "9"; pages: "3125-30"
COMMENT     Sequence Label: tdEos-VSVG-16
FEATURES             Location/Qualifiers
     source          1..3072
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(46..93,94..1578)
                     /codon_start=1
                     /product="vesicular stomatitis virus G glycoprotein, 
                     thermosensitive tsO45 mutant"
                     /label=VSV-G tsO45
                     /note="thermosensitive variant of VSV-G Orsay"
                     /translation="MKCLLYLAFLFIGVNCKFTIVFPHNQKGNWKNVPSNYHYCPSSSD
                     LNWHNDLIGTALQVKMPKSHKAIQADGWMCHASKWVTTCDFRWYGPKYITHSIRSFTPS
                     VEQCKESIEQTKQGTWLNPGFPPQSCGYATVTDAEAAIVQVTPHHVLVDEYTGEWVDSQ
                     FINGKCSNDICPTVHNSTTWHSDYKVKGLCDSNLISMDITSFSEDGELSSLGKEGTGFR
                     SNYFAYETGDKACKMQYCKHWGVRLPSGVWFEMADKDLFAAARFPECPEGSSISAPSQT
                     SVDVSLIQDVERILDYSLCQETWSKIRAGLPISPVDLSYLAPKNPGTGPVFTIINGTLK
                     YFETRYIRVDIAAPILSRMVGMISGTTTERELWDDWAPYEDVEIGPNGVLRTSSGYKFP
                     LYMIGHGMLDSDLHLSSKAQVFEHPHIQDAASQLPDDETLFFFGDTGLSKNPIEFVEGW
                     FSSWKSSIASFFFIIGLIIGLFLVLRVGIYLCIKLKHTKKRQIYTDIEMNRLG"
     CDS             1633..2310
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="MSAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     CDS             2359..3036
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="SAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVKE
                     GGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIARN
                     DITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLLE
                     GNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPDN
                     ARR"
ORIGIN
        1 acgtcagatc cgctagcgct accggactca gatctcgccg ccaccatgaa gtgccttttg
       61 tacttagctt ttttattcat cggggtgaat tgcaagttca ccatagtttt tccacacaac
      121 caaaaaggaa actggaaaaa tgttccttcc aattaccatt attgcccgtc aagctcagat
      181 ttaaattggc ataatgactt aataggcaca gccttacaag tcaaaatgcc caagagtcac
      241 aaggctattc aagcagacgg ttggatgtgt catgcttcca aatgggtcac tacttgtgat
      301 ttccgctggt acggaccgaa gtatataaca cattccatcc gatccttcac tccatctgta
      361 gaacaatgca aggaaagcat tgaacaaacg aaacaaggaa cttggctgaa tccaggcttc
      421 cctcctcaaa gttgtggata tgcaactgtg acggatgctg aagcagcgat tgtccaggtg
      481 actcctcacc atgtgcttgt tgatgaatac acaggagaat gggttgattc acagttcatc
      541 aacggaaaat gcagcaatga catatgcccc actgtccata actccacaac ctggcattcc
      601 gactataagg tcaaagggct atgtgattct aacctcattt ccatggacat cacctccttc
      661 tcagaggacg gagagctatc atccctagga aaggagggca cagggttcag aagtaactac
      721 tttgcttatg aaaccggaga caaggcctgc aaaatgcagt actgcaagca ttggggagtc
      781 agactcccat caggtgtctg gttcgagatg gctgataagg atctctttgc tgcagccaga
      841 ttccctgaat gcccagaagg gtcaagtatc tctgctccat ctcagacctc agtggatgta
      901 agtctcattc aggacgttga gaggatcttg gattattccc tctgccaaga aacctggagc
      961 aaaatcagag cgggtcttcc catctctcca gtggatctca gctatcttgc tcctaaaaac
     1021 ccaggaaccg gtcctgtctt taccataatc aatggtaccc taaaatactt tgagaccaga
     1081 tacatcagag tcgatattgc tgctccaatc ctctcaagaa tggtcggaat gatcagtgga
     1141 actaccacag aaagggaact gtgggatgac tgggctccat atgaagacgt ggaaattgga
     1201 cccaatggag ttctgaggac cagttcagga tataagtttc ctttatatat gattggacat
     1261 ggtatgttgg actccgatct tcatcttagc tcaaaggctc aggtgtttga acatcctcac
     1321 attcaagacg ctgcttcgca gcttcctgat gatgagactt tatttttttt tggtgatact
     1381 gggctatcca aaaatccaat cgagtttgta gaaggttggt tcagtagttg gaagagctct
     1441 attgcctctt ttttctttat catagggtta atcattggac tattcttggt tctccgagtt
     1501 ggtatttatc tttgcattaa attaaagcac accaagaaaa gacagattta tacagacata
     1561 gagatgaacc gacttggaaa cagaattctg cagtcgacgg taccgcgggc ccgggatcca
     1621 ccggtcgcca ccatgagtgc gattaagcca gacatgaaga tcaacctccg tatggaaggc
     1681 aacgtaaacg ggcaccactt tgtgatcgac ggagatggta caggcaagcc ttttgaggga
     1741 aaacagagta tggatcttga agtcaaagag ggcggacctc tgccttttgc ctttgatatc
     1801 ctgaccactg cattccatta cggcaacagg gtattcgccg aatatccaga ccacatacaa
     1861 gactatttta agcagtcgtt tcctaagggg tattcgtggg aacgaagctt gactttcgaa
     1921 gacgggggca tttgcattgc cagaaacgac ataacaatgg aaggggacac tttctataat
     1981 aaagttcgat ttcacggtgt aaactttccc gccaatggtc cagttatgca gaagaagacg
     2041 ctgaaatggg agccctccac tgagaaaatg tatgtgcgtg atggagtgct gactggtgat
     2101 attcgcatgg ctttgttgct tgaaggaaat gcccattacc gatgtgactt cagaactact
     2161 tacaaagcta aggagaaggg tgtcaagtta ccaggctacc actttgtgga ccactgcatt
     2221 gagattttaa gccatgacaa agattacaac aaggttaagc tgtatgagca tgctgttgct
     2281 cattctggat tgcctgacaa tgccagacga tccggtagcg gtcacggtac tggttctact
     2341 ggttctggtt cttctgagag tgcgattaag ccagacatga agatcaacct ccgtatggaa
     2401 ggcaacgtaa acgggcacca ctttgtgatc gacggagatg gtacaggcaa gccttttgag
     2461 ggaaaacaga gtatggatct tgaagtcaaa gagggcggac ctctgccttt tgcctttgat
     2521 atcctgacca ctgcattcca ttacggcaac agggtattcg ccgaatatcc agaccacata
     2581 caagactatt ttaagcagtc gtttcctaag gggtattcgt gggaacgaag cttgactttc
     2641 gaagacgggg gcatttgcat tgccagaaac gacataacaa tggaagggga cactttctat
     2701 aataaagttc gatttcacgg tgtaaacttt cccgccaatg gtccagttat gcagaagaag
     2761 acgctgaaat gggagccctc cactgagaaa atgtatgtgc gtgatggagt gctgactggt
     2821 gatattcgca tggctttgtt gcttgaagga aatgcccatt accgatgtga cttcagaact
     2881 acttacaaag ctaaggagaa gggtgtcaag ttaccaggct accactttgt ggaccactgc
     2941 attgagattt taagccatga caaagattac aacaaggtta agctgtatga gcatgctgtt
     3001 gctcattctg gattgcctga caatgccaga cgataagcgg ccgcgactct agatcataat
     3061 cagccatacc ac
//
